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Registros recuperados: 55
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Molecular epidemiology of the human group A rotavirus in the Paraná State, Brazil BABT
Santos,Jucélia Stadinicki dos; Alfieri,Alice Fernandes; Leite,José Paulo Gagliardi; Skraba,Irene; Alfieri,Amauri Alcindo.
From January/2000 to December/2003, 550 diarrheic fecal samples from the children and adults were collected in several geographical regions of Paraná State, Brazil. The enzyme immunoassay showed 120 (21.8%) samples positive for the group A rotaviruses. One hundred and fourteen samples were genotyped by multiplex-nested-PCR assay. The highest frequency (77.5%) of the positive samples (n=93) was observed in the children under 5 years old. Rotavirus diarrhea was more frequent in the cold and dry seasons of the four evaluated years. The most frequent genotypes were: G1 (50.9%), G4 (9.6%), G9 (7.0%), G2 (1.7%), G3 (0.9%), P[ 8] (71.9%), and P[ 4] (3.5%). The P[ 8] G1 (46.5%) and P[ 8] G4 (9.6%) were the main combinations found to P and G genotypes. The mixed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diarrhea; Rotavirus; Diagnostic; PAGE; RT-PCR; Genotypes.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200008
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A Rapid and Reliable Method for Molecular Detection of Fusarium guttiforme, the Etiological Agent of Pineapple Fusariosis BABT
Carnielli-Queiroz,Lorena; Fernandes,Patricia Machado Bueno; Fernandes,Antônio Alberto Ribeiro; Ventura,José Aires.
Abstract Pineapple (Ananas comosus var. comosus) fusariosis is an economically important fungal disease affecting the plant and its fruit. A rapid and reliable diagnosis is the base of integrated disease management practices. Fusariosis has resulted in quarantines for pineapple products in Central America, Africa and Asia. Difficulties diagnosing and correctly identifying the fungus Fusarium guttiforme, agent of the pineapple fusariosis, have led to the search for new methodologies, and for this we developed a new reliable molecular method to detect it. For diagnostic purposes, real-time PCR of elongation factor gene 1-α (ef1) was used to rapidly, specifically and sensitively diagnose F. guttiforme. A pathogenicity test was conducted with slips of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diagnostic; Diseases; Quarantine; Real-time PCR.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100219
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Development and Evaluation of an Indirect ELISA: Serological Survey to Detect Specific Antibodies to Bovine Herpesvirus 4 BABT
Krüger,Ernesto Renato; Penha,Tânia Regina; Hummelgen,Fernanda Cristina; Agottani,Jorge Bacila; Reva,Demétrio; Gonçalves,Robson; Thomaz-Soccol,Vanete.
ABSTRACTThe aim of this work was to develop and evaluate an indirect enzyme-linked immunosorbent assay (ELISA) and made a serological screening for specific antibodies to BoHV-4. Bovine serum samples were collected from different Brazilian states and evaluated for the presence of antibodies for BoHV-4 and BoHV-1. The serological results obtained showed that the indirect ELISA assay could be applied for the detection of specific antibodies for BoHV-4. The ELISA test allowed concluding that BoHV-4 is present in bovines in every Brazilian state from which serum samples were collected. The ELISA assay here standardized proved to be useful for the epidemiological studies and showed a positivity range from 1.8 to 66% in Brazil.
Tipo: Info:eu-repo/semantics/article Palavras-chave: BoHV-4; Serology; Diagnostic; Cattle; Epidemiology.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500725
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PET and PET/CT imaging for the earliest detection and treatment of colorectal carcinoma BABT
Carter,Kevin; Kotlyarov,Eduard.
Approximately 150,000 new cases of colorectal cancer are diagnosed each year with the life time risk of developing colon caner in developed nations being 4.6% in men and 3.2% in women. Screening patients is essential early detection of colon carcinoma to aid in complete resection. Unfortunately current screening methods carry with them poor patient compliance. PET and PET/CT may be a significant part of this screening solution. The authors reviewed and analyzed the English language articles and case reports identified on Medline during the last 10 years. PET and PET/CT results for colorectal carcinoma were tabulated and presented for the fifth Scientific Meeting of the Brazilian Society of Nuclear Biosciences. Though most studies have been retrospective...
Tipo: Info:eu-repo/semantics/article Palavras-chave: PET and PET/CT; Colorectal cancer; Diagnostic.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700025
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Rapid detection of Vancomycin-Resistant Enterococci (VRE) in rectal samples from patients admitted to intensive care units BJID
d'Azevedo,Pedro Alves; Santiago,Kelly Aline de Souza; Furtado,Guilherme Henrique Campos; Xavier,Diego Batista; Pignatari,Antonio Carlos Campos; Titze-de-Almeida,Ricardo.
The reduction in time required to identify vancomycin-resistant enterococci (VRE) has gained increased importance during hospital outbreaks. In the present study, we implemented a laboratory protocol to speed up the VRE screening from rectal samples. The protocol combines a medium for selective VRE isolation (VREBAC®, Probac, São Paulo) and a multiplex PCR for detection and identification of vanA and vanB resistance genes. The screening performance was analyzed in 114 specimens collected from four intensive care units. The swabs were collected at two periods: (1) during a VRE outbreak (February 2006, n=83 patients) and (2) at the post-outbreak period, after adoption of infection control measures (June 2006, n=31 patients). Forty-one/83 VRE (49.4%) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterococcus; VRE; Vancomycin; Diagnostic; PCR.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000400010
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Comparison of PCR-based methods for the simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in clinical samples BJID
de Filippis,Ivano; Andrade,Claudia Ferreira de; Caldeira,Nathalia; Azevedo,Aline Carvalho de; Almeida,Antonio Eugenio de.
Abstract Background Several in-house PCR-based assays have been described for the detection of bacterial meningitis caused by Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenzae from clinical samples. PCR-based methods targeting different bacterial genes are frequently used by different laboratories worldwide, but no standard method has ever been established. The aim of our study was to compare different in-house and a commercial PCR-based tests for the detection of bacterial pathogens causing meningitis and invasive disease in humans. Methods A total of 110 isolates and 134 clinical samples (99 cerebrospinal fluid and 35 blood samples) collected from suspected cases of invasive disease were analyzed. Specific sets of primers...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diagnostic; Neisseria meningitidis; Streptococcus pneumoniae; Haemophilus influenzae.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000400335
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Detection and differentiation of Entamoeba histolytica and Entamoeba dispar in clinical samples through PCR-denaturing gradient gel electrophoresis BJMBR
López-López,P.; Martínez-López,M.C.; Boldo-León,X.M.; Hernández-Díaz,Y.; González-Castro,T.B.; Tovilla-Zárate,C.A.; Luna-Arias,J.P..
Amebiasis is one of the twenty major causes of disease in Mexico; however, the diagnosis is difficult due to limitations of conventional microscopy-based techniques. In this study, we analyzed stool samples using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to differentiate between Entamoeba histolytica (pathogenic) and E. dispar (non-pathogenic). The target for the PCR amplification was a small region (228 bp) of the adh112 gene selected to increase the sensitivity of the test. The study involved 62 stool samples that were collected from individuals with complaints of gastrointestinal discomfort. Of the 62 samples, 10 (16.1%) were positive for E. histolytica while 52 (83.9%) were negative. No sample was positive for E....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amebiasis; Neglected diseases; Diagnostic; PCR; DGGE; Adh112 gene.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400607
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Leptospirosis in slaughtered sows: serological and histopathological investigation BJM
Delbem,Ádina Cléia Botazzo; Freitas,Julio Cesar de; Bracarense,Ana Paula F. R. L.; Müller,Ernst Eckehardt; Oliveira,Rosângela Claret de.
Swine leptospirosis is recognized world wide as an important cause of reproductive failure. Serum and histo-pathological examinations have been constantly used to diagnose this disease. This study was carried out on slaughtered sows from Northern Paraná State to compare serum findings with kidney, liver, ovary and uterus histo-pathological examinations. The microscopic serum-agglutination carried out on 36 animals detected 24 with titles > 100 and 12 negative (< 100). The histopathological examinations made on livers, kidneys, ovaries and uterus stained with hematoxylin-eosin, were carried out on 16 randomly chosen animals, 11 from the groups with > 100 serological titles and five from the negative group. The results showed that all the 16 animals...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospira; Swine; Diagnostic.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200016
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Mycoplasmas hyorhinis in different regions of cuba: diagnosis BJM
Lobo,Evelyn; Poveda,Carlos; Suarez,Alejandro; Hernández,Yenney; Ramírez,Ana; Poveda,José B..
M. hyorhinis is considered one of the etiological agents of arthritis in sucking pigs, but recently as seen, some strains can produce pneumonia that could not be distinguished from the mycoplasmosis caused by M. hyopneumoniae. The study was conducted to research the presence of Mycoplasma hyorhinis (M. hyorhinis ) in different regions of the country from exudates of pig lungs with typical EP lesions. Exudates from 280 pig lungs with typical EP lesions were studied using molecular techniques such as PCR, real time PCR and amplification of the 16S-23S rRNA. It was detected that the 66% of the samples studied resulted positive to M. hyorhinis, and the presence of this species was detected in all the provinces. Amplification and studies on the intergenic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: PCR; Mycoplasma; Diagnostic; Enzootic pneumonia.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200039
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Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic tests BJM
Cargnelutti,Juliana Felipetto; Brum,Mário Celso Sperotto; Weiblen,Rudi; Flores,Eduardo Furtado.
West Nile virus (WNV) envelope glycoproteins preM/E were stably expressed in baby hamster kidney cells and tested as antigen in a fluorescent antibody assay for WNV antibodies. Sera from horses, mice and chicken immunized with an inactivated WNV vaccine and, less consistently, sera from horses acutely infected with WNV, reacted specifically with viral antigens present in preM/E-expressing cells.
Tipo: Info:eu-repo/semantics/article Palavras-chave: West Nile virus; WNV; M/E glycoproteins; Stable expression; Diagnostic.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300040
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Virus isolation in cell culture for confirmatory diagnostic of rabies in bovine specimens Ciência Rural
Kanitz,Fabio Adriano; Cargnelutti,Juliana Felipetto; Weiblen,Rudi; Batista,Helena Beatriz de Carvalho Ruthner; Flores,Eduardo Furtado.
ABSTRACT: This study investigated the suitability of virus isolation (VI) in mouse neuroblastoma cells (N2A) and baby hamster kidney cells (BHK-21) as a confirmatory test for diagnosis of bovine rabies. Fourty-eight brain samples from cattle suspected of rabies were initially submitted to fluorescent antibody test (FAT) and mouse inoculation test (MIT) for routine diagnostic. Subsequently, these specimens were submitted to three protocols of VI in each cell line: a single 24h or 72h passage (T1, T2), or three 48h passages (T3). The FAT and MIT combined detected 32/48 positive samples, from which MIT detected 32 and FAT 31. The average time required for final MIT results was 12.3 days (8 - 21). VI in BHK-21 cells provided definitive, positive results in...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Rabies; Cattle; Outbreaks; Diagnostic; Virus isolation.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015001202193
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Feline subcutaneous pythiosis Ciência Rural
Soares,Luciana Maria Curtio; Schenkel,Diego Montagner; Rosa,Janaina Marcela Assunção; Azevedo,Ludmila Silva; Tineli,Tainara Renata; Dutra,Valéria; Colodel,Edson Moleta; Pescador,Caroline Argenta.
ABSTRACT: Pythiosis in felines is a rare disease associated with the oomycete Pythium insidiosum. The aim of this report was to describe the macroscopic, histopathological, and molecular characteristics of P. insidiosum infection in a 2-year-old cat, with a localized invasive subcutaneous mass. The feline had an increase of volume near the anal region since it was younger. The cat died just after surgery. The necropsy was performed, and samples were collected for histopathological examination. Microscopically, the skin lesion was characterized by necro-eosinophilic dermatitis, panniculitis, and myositis surrounding negatively stained hyphal structures. In the sections stained with GMS, dark brown hyphae were clearly seen inside the affected tissue. They...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pythium insidiosum; Diagnostic; Cat.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000300450
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Rapid serum agglutination and agar gel immunodiffusion tests associated to clinical signs in rams experimentally infected with Brucella ovis Ciência Rural
Nozaki,Cristiane Nakada; Lira,Nair Silva Cavalcanti de; Augusto Filho,Otávio; Azevedo,Hymerson Costa; Rodello,Leandro; Dasso,Maurício Gaudério; Bicudo,Sony Dimas; Antunes,João Marcelo Azevedo de Paula; Megid,Jane.
The purpose of this study was to evaluate the agar gel immunodiffusion and the rapid serum agglutination tests associated to clinical signs in rams experimentally infected with Brucella ovis. The serological profile during the 12 months of infection showed a large fluctuation of antibodies that favors the failure in the diagnostic. The evaluation of tests after the experimental infection allowed to suggest that none of the tests were able to detect the infection throughout the period of study. The study reinforces the importance of considering the clinical signs to support the diagnosis of Brucella ovis infection in rams.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ovine infectious epididimitis; Sheep brucellosis; Diagnostic.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000800023
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Epidemiology of Mycoplasma agalactiae and Mycoplasma mycoides cluster in flocks of northeastern Brazil Ciência Rural
Santos,Sandra Batista dos; Melo,Renata Pimentel Bandeira de; Silva,Luana Thamires Rapôso da; Oliveira,Júnior Mário Baltazar de; Abad,Atzel Candido Acosta; Pinheiro Júnior,José Wilton; Mota,Rinaldo Aparecido.
ABSTRACT: The present study aimed to investigate contagious agalactia (CA) in flocks from Pernambuco State. The study involved 225 goats and 63 ewes; 288 milk samples and 100 vaginal swabs were collected in total. The PCR assays were carried out using specific primers to Mycoplasma agalactiae and the Mycoplasma mycoides cluster. Among the goat’s milk samples,12.0% (27/225) were positive for Mycoplasma agalactiae DNA, while 5.3% (12/225) contained the Mycoplasma mycoides cluster. Of the vaginal swabs taken from goats, 15.4% (12/78) were positive for Mycoplasma agalactiae DNA and 3.8% (3/78) contained the Mycoplasma mycoides cluster. In the case of ewes, 4.3% (1/23) of the milk samples contained Mycoplasma agalactiae DNA, and 7.5% (3/40) were positive for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Contagious agalactia; Flocks; Diagnostic; Epidemiology; Risk factor..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000400452
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Isolation of Leptospira spp from dogs, bovine and swine naturally infected Ciência Rural
Freitas,Julio Cesar de; Silva,Francielle Gibson da; Oliveira,Rosângela Claret de; Delbem,Ádina Cléia Botazzo; Müller,Ernst Eckehardt; Alves,Lucimara Aparecida; Teles,Paulo Sergio.
Leptospira isolation allows definitive diagnosis of the infection. Contamination by microorganisms is one of the inconveniences of the culture. The objective of this study was to describe the isolation of Leptospira from dogs, bovine and swine naturally infected. Urine samples from 14 dogs and three bovines, and kidney, liver, ovary, and uterus body samples from 36 slaughtered sows with unknown health history, were used. The urine and organ samples were cultured in culture medium. Modified Ellinghausen-McCullough-Johnson-Harris medium (EMJH) culture medium was used with addition of 5-fluorouracil, chloramphenicol, vancomycin, nalidixic acid and neomycin. Incubation was performed at 28oC for 24 hours, followed by subculture in modified EMJH without...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospirosis; Diagnostic; Culture; Cattle; Dog; Swine.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000300030
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Diagnóstico da piscicultura na Bacia do Alto Taquari - MS. Infoteca-e
ROTTA, M. A..
O objetivo deste trabalho é a caracterização da situação da piscicultura na bacia do alto Taquari (BAT) a fim de se propor melhorias no seu sistema, viabilizando-o como meio alternativo de renda para os produtores da região. Este estudo foi realizado no mês de abril de 2003 através de levantamento com a aplicação de um questionário aos piscicultores da BAT. Foram obtidas informações para caracterizar os produtores, a propriedade e a produção, além de outros aspectos relevantes à atividade. Dos 24 produtores localizados na BAT, 12 (50%) se encontram em atividade, 10 estão inativos (42%) e em dois deles (8%) não foi possível obter informações. Na BAT a atividade de piscicultura vem sendo realizada principalmente em grandes propriedades, com área média de...
Tipo: Folhetos Palavras-chave: Caracterização; Bacia do Alto Taquari; Mato Grosso do Sul; Brasil; Diagnostic; Production; Scrutiny; Characterization; Taquari river; Wetland; Diagnostico; Levantamento; Peixe; Piscicultura; Produção; Brazil; Pantanal; Fish; Fish culture.
Ano: 2003 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/786918
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Diagnóstico da cultura do algodão em Mato Grosso, 1996. Infoteca-e
FREIRE, E. C.; SANTOS, A. M. dos; ARANTES, E. M.; PARO, M.; FARIAS, F. J. C.; NASCIMENTO, J. L. do; PEDROSA, M. B..
Diagnostico a nivel de Instituicao/municipio; Diagnostico a nivel dos produtores; Perfil do informante/propriedade; Tecnologias de producao de algodao; Informacoes financeiras.
Tipo: Folhetos Palavras-chave: Cultura; Brasil; Mato Grosso; Cuiaba; Cultivation; Diagnostic; Algodão; Diagnostico; Gossypium Hirsutum; Cotton.
Ano: 1997 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/269962
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Diagnóstico e diretrizes do PCBAP relativos à erosão na Bacia do Alto Taquari. Infoteca-e
GALDINO, S.; PELLEGRIN, L. A..
Diagnóstico; Unidades Ambientais Naturais; Planalto do rio Correntes; Chapada das Emas; Planalto do Taquari; Chapada de São Gabriel, Coxim e Campo Grande; Planalto de Maracaju - Campo Grande; Unidades Ambientais Sócio-Econômicas; Itiquira e Alto Garças; Alto Taquari; São Gabriel d?Oeste; Altas bacias dos rios Aquidauana e Taquari; Sonora e Coxim; Costa Rica; Fragilidades Potenciais e Impactos Ambientais; Características geomorfométricas da BAT; Erosão concentrada nas vertentes; Perda potencial de solo; Aporte de sedimentos da BAT para o Pantanal; Unidades de Zoneamento Ambiental; Chapada do rio Correntes; Planalto do Taquari; Chapada das Emas; Chapada de São Gabriel, Coxim e Campo Grande; Planalto de Maracaju; Planalto de Campo Grande; Diretrizes gerais;...
Tipo: Folhetos Palavras-chave: Diretrizes; Bacia do Alto Taquari; Mato Grosso do Sul; Brasil; Erosion; Diagnostic; Diagnostico; Erosão; Impacto Ambiental; Brazil; Pantanal; Environmental impact.
Ano: 2003 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/799063
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Diagnostico da atuacao da Embrapa Algodao perante sua clientela. Infoteca-e
FREIRE, E. C.; CARVALHO, L. P. de; PEDROSA, M. B..
Tipo: Livros Palavras-chave: Embrapa-CNPA; Brasil; Diagnostic; Diagnostico.
Ano: 1999 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/271760
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Coleta de material para diagnóstico das doenças infecciosas que interferem com a reprodução de bovinos. Infoteca-e
PELLEGRIN, A. O.; LEITE, R. C.; LAGE, A. P.; RAVAGLIA, E..
O diagnóstico de problemas reprodutivos de bovinos é uma tarefa árdua e muitas das vezes sem o resultado esperado, em função do grande número de fatores ambientais, de manejo, nutricionais e infeciosos envolvidos. A avaliação dos parâmetros nutricionais, ambientais e de manejo são muito importantes para se diferenciar problemas de causa infecciosa daqueles advindos de deficiências nutricionais ou de falhas no manejo geral ou reprodutivo dos animais. A melhora no diagnóstico de doenças infecciosas e um maior número de informações mais precisas e rápidas para o produtor e para o técnico podem ser conseguidos com emprego de alguns cuidados relativos a coleta e envio de material aos testes requisitados devem ser observados com atenção.
Tipo: Folhetos Palavras-chave: Doença infecciosa; Coleta; Bovine; Infection disease; Diagnostic; Bovino; Diagnostico; Reprodução; Reproduction.
Ano: 2003 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/811093
Registros recuperados: 55
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