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| Registros recuperados: 32 | |
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| Leite,Nadjma Souza; Lima,Aloísio André Bomfim de; Santana,José Carlos Curvelo; Lopes,Francisco Luiz Gumes; Lédo,Ana da Silva; Tambourgi,Elias Basile; Souza,Roberto Rodrigues de. |
| This study aimed to obtain the condition of maximum bromalein activity in different parts of pineapple plants produced in vitro, by micropropagation. The sStems and leaves of Pérola and Imperial cultivar plants were evaluated after three and eight months of in vitro cultivation in Murashige and Skoog medium without growth phytoregulator, macerated in potassium phosphate buffer at different pH values (5.7, 6.7 and 7.7). Total protein and proteolytic activity were determined in the samples after three- and eight-month cultivation periods. For both the cultivars, the best results were obtained at pH 5.7 in extraction media. Pérola cultivar, showed higher bromelain activity in the leaves cultivated in vitro for three months (0.0194U/mL) while in the Imperial... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzymatic activity; Bromelain; Pérola pineapple; Imperial pineapple; Production in vitro. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500002 |
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| Cichoski,Alexandre José; Rotta,Renata Bezerra; Scheuermann,Gerson; Cunha Junior,Anildo; Barin,Juliano Smanioto. |
| Due to the fact that previous studies on the enzymatic activity of Glutathione peroxidase (GSH-Px) diverge widely in their methodology and results, this study aimed to investigate the influence of different analytical conditions on GSH-Px activity in chicken thighs from broilers that were fed different diets with different sources and concentrations of selenium. GSH-Px activity was evaluated six hours after slaughter and 120 days after frozen storage at -18 ºC. The different analytical conditions included time of pre-incubation (0, 10 and 30 minutes), reaction medium, types of substrate (H2O2 (0.72 mM, 7.2 mM, and 72 mM) and Terc-butil hydroperoxide 15 mM), and different buffer concentrations (buffer 1, potassium phosphate 50 mM pH 7.0 + EDTA 1 mM +... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: GSH-Px; Meat chicken; Enzymatic activity; Selenium; Buffers; Substrate. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000400004 |
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| Márquez-López,Anahí; Ramírez-Conejo,Juan David; Chávez-Parga,Ma. Del Carmen; Flores,Dora Cecilia Valencia; Jaramillo,Miguel Angel Zamudio; Rodríguez,Horacio González; González-Hernández,Juan Carlos. |
| Abstract Non-conventional yeasts represent an alternative for microorganisms that are capable to produce the enzyme Tannase (EC 3.1.1.20), which has many applications is the food and pharmaceutical industry as it stands out for its ability to produce ellagic acid out of the enzymatic hydrolysis of ellagitannins. Ellagic acid has been studied in the medical area for its action against various diseases, as is the case of cancer or heart problems. The investigation was conducted to evaluate seven non-conventional yeasts - Debaryomyces hansenii PYC ISA 1510, Debaryomyces hansenii PYC 2968, Candida parapsilosis, Candida utilis, Pichia pastoris, Pichia kluyveri, Issatchenkia terricola - as producers of the enzyme tannase. This was done in mediums of fermentation... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Non-conventional yeasts; Enzymatic activity; Tannase; Ellagic acid; Hydrolysis. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019005031111 |
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| Brandão,Taís Silva de Oliveira; Sena,Amanda Reges de; Teshima,Elisa; David,Jorge Maurício; Assis,Sandra Aparecida. |
| The physiological state of a fruit is closely related to ripening and climatic conditions during the growing period when the fruit undergo changes in color, texture, and flavor. The ripening of the fruit can involve a complex series of biochemical reactions with alteration in enzymes activities, phenols, tannins, and ascorbic acid. The activity of enzymes (carboximethylcellulase, polygalacturonase, and pectinlyase), the total concentration of phenolic compounds, condensed tannins, and vitamin C in five stages of maturation were studied. Significant changes were observed between the maturity stages. The phenolic compounds were higher at green stage (705.01 ± 7.41); tannins were higher at green/purple stage (699.45 ± 0.22). The results showed that the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Syzygium cumini Lam.; Maturity; Total phenolic compounds; Tannins; Vitamin C; Enzymatic activity. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000400004 |
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| Gioda,Carolina Rosa; Pretto,Alexandra; Freitas,Carine de Souza; Leitemperger,Jossiele; Loro,Vania Lucia; Lazzari,Rafael; Lissner,Leandro Ademar; Baldisserotto,Bernardo; Salbego,Joseânia. |
| ABSTRACT: The aim of this study was to verify the activity of some digestive enzymes in four fish species with different feeding habits. Knowledge of these enzymatic activities can help us to understand the species' digestive processes. The species chosen for this study were Ctenopharyngodon idella (herbivore), Rhamdia quelen (omnivore), Leporinus obtusidens (omnivore) and Hoplias malabaricus (carnivore). The digestive tract of these species was divided into four portions to estimate enzymatic activity: stomach, anterior, mid and posterior intestine. Ctenopharyngodon idella had the highest amylase and maltase activities in all portions of the gut, followed by L. obtusidens . The highest trypsin activity was observed in all gut portions of H. malabaricus,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Freshwater fishes; Feeding habits; Enzymatic activity; Amylase; Alkaline protease; Maltase; Trypsin. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300406 |
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| Bravo Rodríguez,Vicente; Jurado Alameda,Encarnación; Martínez Gallegos,Juan Francisco; Reyes Requena,Antonia; García López,Ana Isabel; Sampaio Cabral,Joaquim Manuel; Fernandes,Pedro; Pina da Fonseca,Luis Joaquim. |
| The influence of different commercial surfactants on the enzymatic activity of a commercial alpha-amylase from Bacillus licheniformis (Termamyl 300 L) has been studied. As non-ionic surfactants, alkyl polyglycosides (Glucopon® 215, Glucopon® 600 and Glucopon® 650) were studied, as were fatty alcohol ethoxylates (Findet 1214N/23 and Findet 10/15), and nonyl phenol ethoxylate (Findet 9Q/21.5NF). Also, an anionic surfactant, linear alkyl benzene sulfonate (LAS) was assayed. In general, none of the non-ionic surfactants studied, except Findet 10/15, vary substantially the enzymatic activity. Findet 10/15 has the strongest hydrophobic character and reduces the enzymatic activity more significantly the greater its concentration. Regarding LAS, this surfactant... |
| Tipo: Journal article |
Palavras-chave: Alpha-amylase; Alkyl polyglycosides; Enzymatic activity; Fatty alcohol ethoxylates; Linear alkyl benzene sulfonate; Nonyl phenol ethoxylate. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500012 |
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| Wang,Nan; Shi,Haifeng; Yao,Qin; Zhou,Yang; Kang,Lequn; Chen,Huiqin; Chen,Keping. |
| Alcohol dehydrogenases (ADH) are a class of enzymes that catalyze the reversible oxidation of alcohols to corresponding aldehydes or ketones, by using either nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP), as coenzymes. In this study, a short-chain ADH gene was identified in Bombyx mori by 5'-RACE PCR. This is the first time the coding region of BmADH has been cloned, expressed, purified and then characterized. The cDNA fragment encoding the BmADH protein was amplified from a pool of silkworm cDNAs by PCR, and then cloned into E. coli expression vector pET-30a(+). The recombinant His-tagged BmADH protein was expressed in E. coli BL21 (DE3), and then purified by metal chelating affinity chromatography. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 5'-RACE PCRADH; Enzymatic activity; Recombinant protein. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200013 |
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| Catão,Hugo Cesar RM; Gomes,Luiz Antonio Augusto; Guimarães,Renato M; Fonseca,Pedro Henrique F; Caixeta,Franciele; Galvão,Alexandre G. |
| ABSTRACT Dormancy in lettuce seeds is a process not yet fully understood. High storage temperatures can cause seed dormancy promoting physiological and enzymatic changes. The goal of this study was to investigate the influence of storage period and environments on the quality and dormancy of different lettuce cultivars. We also investigated the biochemical changes. A completely randomized experimental design was used in a 4x8x3 factorial arrangement to evaluate physiological quality on different storage periods (30, 60, 90 and 120 days) and environments (15, 25 and 35°C) of seeds from eight lettuce cultivars (Everglades, Babá de Verão, Elisa, Luiza, Grand Rapids, Hortência, Salinas 88 and Rubete). The biochemical activity was accessed by tetrazolium test... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lactuca sativa; Thermotolerance; Thermoinhibition; Dormancy; Enzymatic activity. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000100118 |
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| Kim, S; Hoppe, H. |
| A rapid and sensitive method to detect the extracellular enzymatic activity of bacteria colonies grown on agar plates is described. Selective agar media supplemented with protein, starch, chitin, Tween-80, etc. are conventionally used to detect biochemical properties of bacteria. It has been experimentally demonstrated with bacteria pure cultures that fluorogenic Methylumbelliferyl (MUF)-substrates are excellent substrate analogues for normally occurring polymers. Based on MUF-substrate hydrolysis the new method provides reliable qualitative estimates of extracellular enzymatic properties of bacteria within minutes using pure cultures as well as agar plates prepared for colony counts. |
| Tipo: Text |
Palavras-chave: Bacteria; Microbiological culture; Biochemical analysis; Enzymatic activity; Analytical techniques. |
| Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-959.pdf |
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| Galgani, Francois; Benyamin, Y; Vanworhmoudt, A; Cecaldi, J. |
| Proteolytic enzymes of hepatopancreas of six species of peneids (Penaeus kerathurus, P. japonicus, P. monodon, P. stylirostris, P. merguiensis and P. vannamei ) have been studied. Some of their physiological variations have been characterised. Increase in acclimation temperature of crustaceans enhances increase of specific activities of proteases. Variations of salinity does not modify specific activities, nor quantities of trypsin, nor electrophoretic patterns of these different proteases. |
| Tipo: Text |
Palavras-chave: Penaeus kerathurus; Penaeidae; Malacostraca; Diets; Growth; Larval development; Life cycle; Acclimation; Environmental effects; Digestive glands; Enzymatic activity; Salinity effects; Temperature effects; Enzymes; Endocrinology; Digestion; Développement larvaire; Croissance; Cycle d'intermue; Carboxypeptidase A and B; Leucine aminopeptidase; Chymotrypsin; Trypsine; Enzymes digestives; Homarus ganmarus; Palaemon serratus; P. vannamei; P. merguiensis; P. stylirostris; P. monodon; P. japonicus; Penaeus keratkurus. |
| Ano: 1983 |
URL: http://archimer.ifremer.fr/doc/1983/acte-1195.pdf |
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| Durand, Patrick. |
| Different parameters have been followed regularly at various times of anchovy (Engraulis encrasicholus ) ripening. Concentration in NaCl and water content as well as values of water activity and pH are constant and remain quite stable during the entire study period. The relatively acid pH value and the quite low measured water activity level created good conditions for preventing microbial putrefaction at room temperature. The free amino acids and non proteinaceous nitrogen increase indicated the modifications occurring in muscular proteins. These changes resulted in an important proteolytic activity modulated by the medium conditions, especially; pH, temperature and ionic strength. The enzymatic activity slowed down regularly with time but was not... |
| Tipo: Text |
Palavras-chave: Enzymatic activity; Nitrogen compounds; PH; Biochemical composition; Cured products. |
| Ano: 1981 |
URL: http://archimer.ifremer.fr/doc/1981/publication-1889.pdf |
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| Castro-ruiz, Diana; Mozanzadeh, Mansour Torfi; Fernández-méndez, Christian; Andree, Karl B.; García-dávila, Carmen; Cahu, Chantal; Gisbert, Enric; Darias, Maria J.. |
| The aim of the study was to evaluate the functional ontogeny of the digestive system of Pseudoplatystoma punctifer through the analysis of the activity of the main intestinal (alkaline phosphatase, aminopeptidase N, maltase and leucine-alanine peptidase), pancreatic (trypsin, chymotrypsin, total alkaline proteases, bile-salt activated lipase and amylase) and gastric (pepsin) enzymes. Larvae were raised in triplicate in a recirculation system from 4 to 27 days post fertilization (dpf) at an initial density of 90 larvae L−1, 27.8 ± 0.7 °C and 0 L: 24D photoperiod. Larvae were fed from 4 to 17 dpf with Artemia nauplii and weaned onto an experimentally formulated feed (crude protein content ~ 45%; crude fat content ~ 10%; crude carbohydrate ~ 8%) within... |
| Tipo: Text |
Palavras-chave: Pseudoplatystoma punctifer; Ontogeny; Enzymatic activity; Digestive system; Brush border; Pancreatic enzymes; Gastric enzymes. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00479/59082/61723.pdf |
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| Meyerreil, L; Graf, G. |
| The seasonal development of benthic communities was followed in a coastal marine sediment of the Kiel Bight (Baltic Sea; FRG). Total benthic biomass, (ATP), bacterial biomass, overall benthic activity (heat production), and enzymatic decomposition rates of carbohydrate (d-amylase activity) followed a seasonal cycle strongly related to certain events in the sediment. Among these, the input of the phytoplankton blooms in autumn and spring, the accumulation of organic material during winter, and changes in the physical characteristics of the sediment turned out to be most important for the development of benthic biomass and activity. Processes within the benthic community occurred in very short time scales (within days). |
| Tipo: Text |
Palavras-chave: Bacteria; Organic matter; Sediments; Bioenergetics; Enzymatic activity; Biomass. |
| Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-944.pdf |
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| Registros recuperados: 32 | |
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