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| Registros recuperados: 39 | |
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| MONTEIRO, A. C. A.; RESENDE, M. L. V. de; VALENTE, T. C. T.; RIBEIRO JUNIOR, P. M.; PEREIRA, V. F.; COSTA, J. R. da; SILVA, J. A. G. da. |
| Phosphites (Phi) are inorganic salts of phosphorous acid that have been used as resistance elicitors to activate mechanisms underlying plant defence. This study assessed the effectiveness of manganese phosphite (MnPhi) for the management of rust (Hemileia vastatrix) on coffee seedlings. The effect of this compound on the induction of resistance was also measured by the expression of defence-related genes such as POX (peroxidase), CAT (catalase), GLU (b-1.3-glucanase) and PAL (phenylalanine ammonia-lyase) and by the activity of defence enzymes such as ascorbate peroxidase (APX), superoxide dismutase (SOD) and polyphenol oxidase (PPO). Foliar sprayings of MnPhi on coffee seedlings provided 63% control of rust severity. This compound induced defence responses... |
| Tipo: Artigo de periódico |
Palavras-chave: Ferrugem do café; Enzymatic activity; Rust.; Resistance elicitor; Café; Fungo; Coffea Arábica.; Gene expression.. |
| Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1067414 |
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| SANTOS, J. V. DOS; BENTO, L. R.; BRESOLIN, J. D.; OLIVEIRA, P. P. A.; BERNARDI, A. C. de C.; PEZZOPANE, J. R. M.; MENDES, I. de C.; MARTIN NETO, L.. |
| The demand for food has intensified at the rate that the world population has grown, requiring production models capable of meeting world needs, through high productivity and conciliating with sustainable practices. In this scenario, the silvopastoral system (SPS) has been introduced as an alternative to current production models. Thus, this study aimed to evaluate the current soil condition in an SPS using chemical and microbiological parameters as indicators of soil quality, using a native forest and degraded pasture as a positive and negative reference, respectively. For this, soil samples were collected at a depth of 0-10 cm, and the total carbon and B-glycosidase activity (BGL) were determined. Both SPS sub-areas, in the tree rows (SPS-R) and between... |
| Tipo: Anais e Proceedings de eventos |
Palavras-chave: Enzymatic activity; B glycosidase; Soil quality. |
| Ano: 2021 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1136019 |
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| SILVA, C. F. da; PEREIRA, M. G.; RODRIGUES, L. A. da S.; TEODORO, S. A.; MARCELO ANTONIOL FONTES; GAIA-GOMES, J. H.; SILVA, E. M. R. da. |
| Water erosion is one of the main forms of soil degradation, causing economic, environmental, and social damage. This study evaluated the effects of different formation stages of gullies (initial - IG; juvenile- JG; mature - MG; and senile - SG) on the chemical, organic carbon and microbiological attributes of soil, using a secondary forest (SF) and two areas of pasture as references in the ?Mar de Morros? environment of the Brazilian Atlantic Forest biome. Soil samples (depth 0?0.05 m) were collected at the end of the rainy and dry seasons. Gullies in different stages of formation promote a decrease in soil fertility and modification of microbiological attributes, particularly when compared with secondary forest areas. Reductions of over 60% in total... |
| Tipo: Artigo de periódico |
Palavras-chave: Enzymatic activity; Soil microbial biomass; Floresta Atlântica; Soil organic carbon; Oxisols; Water erosion. |
| Ano: 2021 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137991 |
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| DEUS, K. E. de; LANNA, A. C.; ABREU, F. R. M.; SILVEIRA, R. D. D.; PEREIRA, W. J.; BRONDANI, C.; VIANELLO, R. P.. |
| Drought is a major cause of reduced yield in upland rice farming in many regions of the world and cause an increased production of reactive oxygen species (ROS), followed by activation of a complex antioxidant system. In this study, the superoxide dismutase (SOD) enzymatic activity and the gene expression levels of eight isoforms of SOD were evaluated in drought-tolerant and drought-sensitive varieties of upland rice, Oryza sativa japonica (the Primavera and Douradão cultivars), including shoot and root tissues in two stages of plant development, grown under normal and restricted irrigation. The SOD activity was determined by spectrophotometric method and the gene expression by qPCR experiments. In the tolerant cultivar, SOD enzymatic activity increased... |
| Tipo: Artigo de periódico |
Palavras-chave: Enzymatic activity; Arroz; Oryza sativa; Resistencia a seca; Rice; Water stress; Oxidative stress; Gene expression; Superoxide dismutase. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1037521 |
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| SILVA, R. de C. B. da; ARAÚJO, M. do N.; ORNELLAS, F. L. S.; DANTAS, B. F.. |
| Given that watermelon is a crop widespread around the world, there is considerable interest in verifying how its seeds physiologically behave under unfavorable temperature conditions. This study aimed to evaluate the biochemical changes mobilization and reserves degradation, as well as the enzyme activity, during seed germination and initial growth of watermelon seedlings subjected to thermal stress, using temperature, germination percentage, germination rate and relative germination frequency over the incubation time as evaluation parameters. The experimental design was completely randomized, in a 5 x 3 factorial scheme, with five temperatures (17 ºC, 20 ºC, 25 ºC, 30 ºC and 35 ºC) and three cultivars (Charleston Gray, Fairfax and Crimson Sweet), with 4... |
| Tipo: Artigo de periódico |
Palavras-chave: Enzymatic activity; Melancia; Semente; Germinação; Fisiologia; Atividade Enzimática; Citrullus Lanatus; Germination. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1090900 |
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| Leite,Nadjma Souza; Lima,Aloísio André Bomfim de; Santana,José Carlos Curvelo; Lopes,Francisco Luiz Gumes; Lédo,Ana da Silva; Tambourgi,Elias Basile; Souza,Roberto Rodrigues de. |
| This study aimed to obtain the condition of maximum bromalein activity in different parts of pineapple plants produced in vitro, by micropropagation. The sStems and leaves of Pérola and Imperial cultivar plants were evaluated after three and eight months of in vitro cultivation in Murashige and Skoog medium without growth phytoregulator, macerated in potassium phosphate buffer at different pH values (5.7, 6.7 and 7.7). Total protein and proteolytic activity were determined in the samples after three- and eight-month cultivation periods. For both the cultivars, the best results were obtained at pH 5.7 in extraction media. Pérola cultivar, showed higher bromelain activity in the leaves cultivated in vitro for three months (0.0194U/mL) while in the Imperial... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzymatic activity; Bromelain; Pérola pineapple; Imperial pineapple; Production in vitro. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500002 |
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| Cichoski,Alexandre José; Rotta,Renata Bezerra; Scheuermann,Gerson; Cunha Junior,Anildo; Barin,Juliano Smanioto. |
| Due to the fact that previous studies on the enzymatic activity of Glutathione peroxidase (GSH-Px) diverge widely in their methodology and results, this study aimed to investigate the influence of different analytical conditions on GSH-Px activity in chicken thighs from broilers that were fed different diets with different sources and concentrations of selenium. GSH-Px activity was evaluated six hours after slaughter and 120 days after frozen storage at -18 ºC. The different analytical conditions included time of pre-incubation (0, 10 and 30 minutes), reaction medium, types of substrate (H2O2 (0.72 mM, 7.2 mM, and 72 mM) and Terc-butil hydroperoxide 15 mM), and different buffer concentrations (buffer 1, potassium phosphate 50 mM pH 7.0 + EDTA 1 mM +... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: GSH-Px; Meat chicken; Enzymatic activity; Selenium; Buffers; Substrate. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000400004 |
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| Márquez-López,Anahí; Ramírez-Conejo,Juan David; Chávez-Parga,Ma. Del Carmen; Flores,Dora Cecilia Valencia; Jaramillo,Miguel Angel Zamudio; Rodríguez,Horacio González; González-Hernández,Juan Carlos. |
| Abstract Non-conventional yeasts represent an alternative for microorganisms that are capable to produce the enzyme Tannase (EC 3.1.1.20), which has many applications is the food and pharmaceutical industry as it stands out for its ability to produce ellagic acid out of the enzymatic hydrolysis of ellagitannins. Ellagic acid has been studied in the medical area for its action against various diseases, as is the case of cancer or heart problems. The investigation was conducted to evaluate seven non-conventional yeasts - Debaryomyces hansenii PYC ISA 1510, Debaryomyces hansenii PYC 2968, Candida parapsilosis, Candida utilis, Pichia pastoris, Pichia kluyveri, Issatchenkia terricola - as producers of the enzyme tannase. This was done in mediums of fermentation... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Non-conventional yeasts; Enzymatic activity; Tannase; Ellagic acid; Hydrolysis. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019005031111 |
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| Brandão,Taís Silva de Oliveira; Sena,Amanda Reges de; Teshima,Elisa; David,Jorge Maurício; Assis,Sandra Aparecida. |
| The physiological state of a fruit is closely related to ripening and climatic conditions during the growing period when the fruit undergo changes in color, texture, and flavor. The ripening of the fruit can involve a complex series of biochemical reactions with alteration in enzymes activities, phenols, tannins, and ascorbic acid. The activity of enzymes (carboximethylcellulase, polygalacturonase, and pectinlyase), the total concentration of phenolic compounds, condensed tannins, and vitamin C in five stages of maturation were studied. Significant changes were observed between the maturity stages. The phenolic compounds were higher at green stage (705.01 ± 7.41); tannins were higher at green/purple stage (699.45 ± 0.22). The results showed that the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Syzygium cumini Lam.; Maturity; Total phenolic compounds; Tannins; Vitamin C; Enzymatic activity. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000400004 |
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| Gioda,Carolina Rosa; Pretto,Alexandra; Freitas,Carine de Souza; Leitemperger,Jossiele; Loro,Vania Lucia; Lazzari,Rafael; Lissner,Leandro Ademar; Baldisserotto,Bernardo; Salbego,Joseânia. |
| ABSTRACT: The aim of this study was to verify the activity of some digestive enzymes in four fish species with different feeding habits. Knowledge of these enzymatic activities can help us to understand the species' digestive processes. The species chosen for this study were Ctenopharyngodon idella (herbivore), Rhamdia quelen (omnivore), Leporinus obtusidens (omnivore) and Hoplias malabaricus (carnivore). The digestive tract of these species was divided into four portions to estimate enzymatic activity: stomach, anterior, mid and posterior intestine. Ctenopharyngodon idella had the highest amylase and maltase activities in all portions of the gut, followed by L. obtusidens . The highest trypsin activity was observed in all gut portions of H. malabaricus,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Freshwater fishes; Feeding habits; Enzymatic activity; Amylase; Alkaline protease; Maltase; Trypsin. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300406 |
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| Bravo Rodríguez,Vicente; Jurado Alameda,Encarnación; Martínez Gallegos,Juan Francisco; Reyes Requena,Antonia; García López,Ana Isabel; Sampaio Cabral,Joaquim Manuel; Fernandes,Pedro; Pina da Fonseca,Luis Joaquim. |
| The influence of different commercial surfactants on the enzymatic activity of a commercial alpha-amylase from Bacillus licheniformis (Termamyl 300 L) has been studied. As non-ionic surfactants, alkyl polyglycosides (Glucopon® 215, Glucopon® 600 and Glucopon® 650) were studied, as were fatty alcohol ethoxylates (Findet 1214N/23 and Findet 10/15), and nonyl phenol ethoxylate (Findet 9Q/21.5NF). Also, an anionic surfactant, linear alkyl benzene sulfonate (LAS) was assayed. In general, none of the non-ionic surfactants studied, except Findet 10/15, vary substantially the enzymatic activity. Findet 10/15 has the strongest hydrophobic character and reduces the enzymatic activity more significantly the greater its concentration. Regarding LAS, this surfactant... |
| Tipo: Journal article |
Palavras-chave: Alpha-amylase; Alkyl polyglycosides; Enzymatic activity; Fatty alcohol ethoxylates; Linear alkyl benzene sulfonate; Nonyl phenol ethoxylate. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500012 |
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| Wang,Nan; Shi,Haifeng; Yao,Qin; Zhou,Yang; Kang,Lequn; Chen,Huiqin; Chen,Keping. |
| Alcohol dehydrogenases (ADH) are a class of enzymes that catalyze the reversible oxidation of alcohols to corresponding aldehydes or ketones, by using either nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP), as coenzymes. In this study, a short-chain ADH gene was identified in Bombyx mori by 5'-RACE PCR. This is the first time the coding region of BmADH has been cloned, expressed, purified and then characterized. The cDNA fragment encoding the BmADH protein was amplified from a pool of silkworm cDNAs by PCR, and then cloned into E. coli expression vector pET-30a(+). The recombinant His-tagged BmADH protein was expressed in E. coli BL21 (DE3), and then purified by metal chelating affinity chromatography. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 5'-RACE PCRADH; Enzymatic activity; Recombinant protein. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200013 |
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| Catão,Hugo Cesar RM; Gomes,Luiz Antonio Augusto; Guimarães,Renato M; Fonseca,Pedro Henrique F; Caixeta,Franciele; Galvão,Alexandre G. |
| ABSTRACT Dormancy in lettuce seeds is a process not yet fully understood. High storage temperatures can cause seed dormancy promoting physiological and enzymatic changes. The goal of this study was to investigate the influence of storage period and environments on the quality and dormancy of different lettuce cultivars. We also investigated the biochemical changes. A completely randomized experimental design was used in a 4x8x3 factorial arrangement to evaluate physiological quality on different storage periods (30, 60, 90 and 120 days) and environments (15, 25 and 35°C) of seeds from eight lettuce cultivars (Everglades, Babá de Verão, Elisa, Luiza, Grand Rapids, Hortência, Salinas 88 and Rubete). The biochemical activity was accessed by tetrazolium test... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lactuca sativa; Thermotolerance; Thermoinhibition; Dormancy; Enzymatic activity. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000100118 |
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| Kim, S; Hoppe, H. |
| A rapid and sensitive method to detect the extracellular enzymatic activity of bacteria colonies grown on agar plates is described. Selective agar media supplemented with protein, starch, chitin, Tween-80, etc. are conventionally used to detect biochemical properties of bacteria. It has been experimentally demonstrated with bacteria pure cultures that fluorogenic Methylumbelliferyl (MUF)-substrates are excellent substrate analogues for normally occurring polymers. Based on MUF-substrate hydrolysis the new method provides reliable qualitative estimates of extracellular enzymatic properties of bacteria within minutes using pure cultures as well as agar plates prepared for colony counts. |
| Tipo: Text |
Palavras-chave: Bacteria; Microbiological culture; Biochemical analysis; Enzymatic activity; Analytical techniques. |
| Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-959.pdf |
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| Registros recuperados: 39 | |
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