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| Registros recuperados: 30 | |
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| GERN, J. C.; SANTOS, L. F. dos; HABU, S.; NOSEDA, M. D.; LIMA, L. F. O. de; SILVA, A. L. L. da; SOCCOL, V. T.; BRANDAO, H. de M.; SOCCOL, C. R.. |
| Background and purpose: Although the activities of polysaccharides such as beta-glucans are well known in Agaricus brasiliensis, there has been little research on their exopolysaccharides and its characterization or scale up studies. The aim of the study was to study the monosaccharide composition of the exopolysaccharide (EPS) produced by Agaricus brasiliensis LPB3 and its production in a stirred tank fermenter. Materials and methods: Agaricus brasiliensis LPB3 was cultured in submerged fermentation in a medium containing glucose, yeast extract, K2HPO4 and MgSO4. Extracellular polysaccharide was precipitated with four volumes of 95% ethanol. For monosaccharide composition, the EPSs were hydrolyzed, derivatized to alditol acetates, and analyzed by gas... |
| Tipo: Separatas |
Palavras-chave: Exopolysaccharide; Agaricus brasiliensis; Scale up; Monosaccharide composition; Stirred tank fermenter; Basidiomycete. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1037830 |
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| PAULO, E. M.; BOFFO, E. F.; BRANCO, A.; VALENTE, A. M. M. P.; MELO, I. S. de; FERREIRA, A. G.; ROQUE, M. R. de A.; ASSIS, S. A. de. |
| ABSTRACT: The genus Leuconostoc belongs to a group of lactic acid bacteria usually isolated from fermented vegetables, which includes species involved in the production of exopolysaccharides (EPS). These biopolymers possess considerable commercial potential. Because of the wide variety of industrial applications of EPS, this study aimed to produce and characterize the native exopolysaccharide strain Leuconostoc pseudomesenteroides R2, which was isolated from cabbage collected in a semi-arid region of Bahia. We employed the following conditions for the production of EPS: 10.7% sucrose, pH 8.2, without agitation and incubation at 28°C for 30 hours. The fermentation broth was treated with ethanol and generated two types of polysaccharide substances (EPS I and... |
| Tipo: Separatas |
Palavras-chave: Exopolysaccharide; Polymer.; Bactéria; Leuconostoc pseudomesenteroides; Biopolymers; Dextran.. |
| Ano: 2012 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/942711 |
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| XU,CHUNPING; YU,JINWEI; ZHAO,SHANSHAN; WU,SHUNGSHUNG; HE,PEIXIN; JIA,XUEWEI; LIU,YANQI; MAO,DUOBIN. |
| ABSTRACT The effect on different three carbon source (i.e. glucose, fructose and sucrose) on production, chemical characterization and antioxidant activity of exopolysaccharide (EPS) produced by Phellinus vaninii Ljup was investigated in this study. Amongst carbon sources examined, glucose and sucrose were favorable for the mycelia growth, while the maximum EPS yield was achieved when sucrose was employed. The predominant carbohydrate compositions in EPSs identified were gluconic acid, glucose, mannose and galactose acid. Then, FT-IR spectral analysis revealed prominent characteristic groups in EPSs. EPSs molecule exist as nearly globular shape form in aqueous solution. The variation also affects antioxidant activities by investigated by using hydroxyl and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phellinus vaninii Ljup; Exopolysaccharide; Carbon source; Antioxidant activity; Composition. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502033 |
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| Paulo,Elinalva M.; Boffo,Elisangela F.; Branco,Alexsandro; Valente,Ângela M.M.P.; Melo,Itamar S.; Ferreira,Antonio G.; Roque,Milton R.A.; Assis,Sandra A. de. |
| The genus Leuconostoc belongs to a group of lactic acid bacteria usually isolated from fermented vegetables, which includes species involved in the production of exopolysaccharides (EPS). These biopolymers possess considerable commercial potential. Because of the wide variety of industrial applications of EPS, this study aimed to produce and characterize the native exopolysaccharide strain Leuconostoc pseudomesenteroides R2, which was isolated from cabbage collected in a semi-arid region of Bahia. We employed the following conditions for the production of EPS: 10.7% sucrose, pH 8.2, without agitation and incubation at 28ºC for 30 hours. The fermentation broth was treated with ethanol and generated two types of polysaccharide substances (EPS I and EPS II).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leuconostoc pseudomesenteroides; Exopolysaccharide; Polymer; Dextran. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200018 |
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| Fusconi,Roberta; Godinho,Mirna Januária Leal; Hernández,Isara Lourdes Cruz; Bossolan,Nelma Regina Segnini. |
| A strain of Gordonia sp. (strain Lc), from landfill leachate-contaminated groundwater was characterized by polyphasic taxonomy and studied for exopolysaccharide (EPS) production. The cells were Gram-positive, catalase-positive, oxidase-negative and non-motile. The organism grew both aerobically and, in anoxic environment, in the presence of NaNO3. Rods occured singly, in pairs or in a typical coryneform V-shaped. The organism had morphological, physiological and chemical properties consistent with its assignment to the genus Gordonia and mycolic and fatty acid pattern that corresponded to those of G. polyisoprenivorans DSM 44302T. The comparison of the sequence of the first 500 bases of the 16S rDNA of strain Lc gave 100% similarity with the type strain of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gordonia polyisoprenivorans; Exopolysaccharide; Groundwater; Landfill. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200014 |
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| Sun,Xinyi; Hao,Long; Ma,Hua; Li,Tong; Zheng,Lan; Ma,Zhao; Zhai,Guoyin; Wang,Liqin; Gao,Shanglong; Liu,Xiaonan; Jia,Mengshi; Jia,Le. |
| The extraction parameters for Pleurotus eryngii SI-02 exopolysaccharide (EPS) produced during submerged culture were optimized using response surface methodology (RSM). The optimum conditions for EPS extraction were predicted to be, precipitation time 20.24 h, ethanol concentration 89.62% and pH 8.17, and EPS production was estimated at 7.27 g/L. The actual yield of EPS under these conditions was 7.21 g/L. The in vitro antioxidant results of the EPS showed that the inhibition effects of EPS at a dosage of 400 mg/L on hydroxyl, superoxide anion and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were 59.63 ± 3.72%, 38.69 ± 2.59%, and 66.36 ± 4.42%, respectively, which were 12.74 ± 1.03%, 8.01 ± 0.56%, and 12.19 ± 1.05% higher than that of butylated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pleurotus eryngii SI-02; Exopolysaccharide; Extraction; Antioxidant activity; In vitro. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400009 |
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| Zhang,Tiehua; Zhang,Chunhong; Li,Shengyu; Zhang,Yanchun; Yang,Zhennai. |
| To analyze the exopolysaccharide (EPS) production by Streptococcus thermophilus ST1, cultures were cultivated in 10% (w/v) reconstituted skim milk under different growth conditions including various temperatures and pHs of growth medium, supplementation of the medium with various carbon sources (glucose, lactose, sucrose, galactose and fructose) and nitrogen source (whey protein concentrate, or WPC). The results showed that most EPS production by strain ST1 was obtained at a temperature (42°C) and pH (6.5) optimal for its growth. Supplementation of the skim milk medium with either carbohydrates or WPC increased both growth and polymer formation by different extents, with sucrose being most effective among the carbon sources tested. Under the optimal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Exopolysaccharide; Streptococcus thermophilus ST1; Skim milk. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400033 |
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| Mikiashvili,Nona A; Isikhuemhen,Omoanghe S; Ohimain,Elijah I. |
| Fourteen strains of Grifola frondosa (Dicks.) S. F. Gray, originating from different regions (Asia, Europe and North America) were tested for lignin degradation, ligninolytic enzyme activities, protein accumulation and exopolysaccharide production during 55 days of cultivation on oak sawdust. Lignin degradation varied from 2.6 to7.1 % of dry weight of the oak sawdust substrate among tested strains. The loss of dry matter in all screened fungi varied between 11.7 and 33.0%, and the amount of crude protein in the dry substrate varied between 0.94 to 2.55%. The strain, MBFBL 596, had the highest laccase activity (703.3 U/l), and the maximum peroxidase activity of 22.6 U/l was shown by the strain MBFBL 684. Several tested strains (MBFBL 21, 638 and 662)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Grifola frondosa; Exopolysaccharide; Laccase; Lignin degradation; Peroxidase. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300031 |
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| Florencia,Frau Silvia. |
| The aim of this study was to compare the rheology of spreadable cheeses elaborated with autochthonous lactic starter cultures without the addition of exopolysaccharide-producing strain in the same starter with exopolysaccharide-producing strain. From a rheological standpoint, both samples were characterized as weak viscoelastic gels and pseudoplastic products. It was concluded that cheese made with exopolysaccharide-producing strain showed smaller G', G", and η* values over the range of frequencies studied and smaller critic stress values than the cheese without exopolysaccharide-producing strain. The results obtained indicate that cheeses without exopolysaccharide-producing strain need to be added with any texture enhancer product. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rheology; Spreadable cheese; Exopolysaccharide. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000200003 |
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| Becker,Sidnei; Soares,Cíntia; Porto,Luismar Marques. |
| Groups of genes that produce exopolysaccharide with a N-acetyl-D-glucosamine monomer are in the genome of several pathogenic bacteria. Chromobacterium violaceum, an opportunistic pathogen, has the operon hmsHFR-CV2940, whose proteins can synthesize such polysaccharide. In this work, multiple alignments among proteins from bacteria that synthesize such polysaccharide were used to verify the existence of amino acids that might be critical for pathogen activity. Three-dimensional models were generated for spatial visualization of these amino acid residues. The analysis carried out showed that the protein HmsR preserves the amino acids D135, D228, Q264 and R267, considered critical for the formation of biofilms and, furthermore, that these amino acids are... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilms; Exopolysaccharide; Chromobacterium violaceum pathogenicity; Comparative genomics. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300031 |
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| Colliec-jouault, Sylvia; Zanchetta, P; Helley, D; Ratiskol, Jacqueline; Sinquin, Corinne; Fischer, A. M.; Guezennec, Jean. |
| Bacterial polysaccharides offer fascinating potential applications for the pharmaceutical industry. Although many known marine bacteria produce exopolysaccharides (EPS), continuation in looking for new polysaccharide-producing micro-organisms is promising. Marine bacteria, isolated from deep-sea hydrothermal vents, have demonstrated their ability to produce in aerobic conditions, unusual EPS. With the aim of discovering biological activities, EPS presenting different structural features were studied. An EPS secreted by Vibrio, diabolicus was evaluated on the restoration of bone integrity in experimental model and was demonstrated to be a strong bone-healing material. Another EPS produced by Alteromonas infernus was modified in order to obtain new... |
| Tipo: Text |
Palavras-chave: Hydrothermal vents; Exopolysaccharides; Bone healing; Biomimetic; Anticoagulant activity; Sources hydrothermales; Exopolysaccharide; Cicatrisation osseuse; Biomimétique; Activité anticoagulante. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-2117.pdf |
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| Toullec, Jordan; Moriceau, Brivaela. |
| Diatom production is mainly supported by the dissolution of biogenic silica (bSiO(2)) within the first 200 m of the water column. The upper oceanic layer is enriched in dissolved and/or colloidal organic matter, such as exopolymeric polysaccharides (EPS) and transparent exopolymeric particles (TEP) excreted by phytoplankton in large amounts, especially at the end of a bloom. In this study we explored for the first time the direct influence of TEP-enriched diatom excretions on bSiO(2) dissolution. Twelve dissolution experiments on fresh and fossil diatom frustules were carried out on seawater containing different concentrations of TEP extracted from diatom cultures. Fresh diatom frustules were cleaned from the organic matter by low ash temperature, and... |
| Tipo: Text |
Palavras-chave: Exopolysaccharide; Diatom excretion; Diatomite; Chaetoceros muelleri; Biogenic silica; BSiO(2) dissolution; TEP. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00616/72795/72270.pdf |
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| Zykwinska, Agata; Marquis, Melanie; Godin, Mathilde; Marchand, Laetitia; Sinquin, Corinne; Garnier, Catherine; Jonchere, Camille; Chedeville, Claire; Le Visage, Catherine; Guicheux, Jerome; Colliec-jouault, Sylvia; Cuenot, Stephane. |
| Articular cartilage is an avascular, non-innervated connective tissue with limited ability to regenerate. Articular degenerative processes arising from trauma, inflammation or due to aging are thus irreversible and may induce the loss of the joint function. To repair cartilaginous defects, tissue engineering approaches are under intense development. Association of cells and signalling proteins, such as growth factors, with biocompatible hydrogel matrix may lead to the regeneration of the healthy tissue. One current strategy to enhance both growth factor bioactivity and bioavailability is based on the delivery of these signalling proteins in microcarriers. In this context, the aim of the present study was to develop microcarriers by encapsulating... |
| Tipo: Text |
Palavras-chave: Exopolysaccharide; Growth factor; Microparticles; Microfluidics; Bioactivity. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00477/58882/61432.pdf |
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| Velasco, C. Ruiz; Baud'Huin, M.; Sinquin, Corinne; Maillasson, M.; Heymann, D.; Colliec-jouault, Sylvia; Padrines, M.. |
| The growth and differentiation of bone cells is controlled by various factors which can be modulated by heparan sulphates. Here, we investigated the effects of an oversulphated exopolysaccharide (OS-EPS) on bone. We compared the effect of this compound with that of a native exopolysaccharide (EPS). Long-term administration of OS-EPS causes cancellous bone loss in mice due, in part, to an increase in the number of osteoclasts lining the trabecular bone surface. No significant difference in cancellous bone volume was found between EPS-treated mice and age-matched control mice, underlying the importance of sulphation in trabecular bone loss. However, the mechanism sustaining this osteoporosis was unclear. To clarify OS-EPS activities, we investigated the... |
| Tipo: Text |
Palavras-chave: Bone metabolism; Bone remodeling; Exopolysaccharide; Glycosaminoglycan; Heparin. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00032/14300/15749.pdf |
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| Registros recuperados: 30 | |
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