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| Registros recuperados: 32 | |
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| Melo-Silva, Merieli; Oliveira, Felipe Rafael; Rosa, Jonathan; Gemelli, Emanuelli; Santos, Lilian; Bueno-Krawczyk, Ana Carolina de Deus. |
| Most changes in water bodies are result of human activities that have the potential to undermine the environmental integrity of aquatic ecosystems. Changes in genetic material can be evidenced by the frequency of nuclear abnormalities in fish blood cells, in response to genotoxic agents even at low concentrations. Thus, we aimed at comparing the frequencies of nuclear abnormalities of fish kept in acclimation, with fish collected in Timbó river (Santa Cruz do Timbó, Santa Catarina State), under low anthropogenic interference, and fish collected in Iguaçu river (União da Vitória, Paraná State), a polluted river. The highest frequency of changes in nuclear morphology of fish erythrocytes was found in the urbanized area around the Iguaçu river, while, the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Micronucleus; Ecotoxicology; Mutagenesis; Pollution. Mutagênese. |
| Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/40669 |
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| Sun,Jia-Long; Zou,Xiao; Liu,Ai-Ying; Xiao,Tang-Fu. |
| In China, Monascus spp., a traditional fungus used in fermentation, is used as a natural food additive. Monascus spp. can produce a secondary metabolite, monacolin K namely, which is proven to be a cholesterol-lowering and hypotensive agent. Hence, recently, many researchers have begun focusing on how to increase the production of monacolin K by Monascus purpureus. In the present study, we investigated the effect of the fungal elicitor and the mutagenesis of UV & LiCl on the amount of monacolin K produced by Monascus purpureus. The fugal elicitor, Sporobolomyces huaxiensis, was isolated from tea leaves and its filtrate was added into the culture filtrate of Monascus purpureus during growth to induct the production of monacolin K. The results showed... |
| Tipo: Journal article |
Palavras-chave: Monascus spp.; Monacolin K; Fungal elicitor; Mutagenesis. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400010 |
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| Ferreira,Maria Izildinha; Rodrigues,Geraldo Stachetti; Domingos,Marisa; Saldiva,Paulo Hilário do N.. |
| The Tradescantia stamen hair mutation assay (Trad-SHM) was employed for assessing the environmental mutagenesis in two areas of São Paulo City. The study sites were defined as follows: 1) Celso Garcia Avenue, an industrial area on the outskirts of the urban center (Belém neighborhood), with an average daily traffic volume of 8,750 vehicles; 2) Doutor Arnaldo Avenue, in downtown area (Pinheiros neighborhood), with a mostly residential and business offices occupation, with an average daily traffic volume of 46,125 vehicles. The negative control (background) site is located in the city of Jaguariúna, 138 km from São Paulo, in an area of low atmospheric pollution. The mutation frequency observed for Belém was similar to the background for clone 4430, whereas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pollution; Mutagenesis; Bioassay; Tradescantia. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200017 |
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| Carvalheiro,César Vinícius; Rocha,Ledyane Dalgallo; Maranho,Leila Teresinha. |
| This study aimed to evaluate the existence of leaf anatomic characteristics in Tabebuia alba changed by air pollutants, which could be used as tool for a bioindication program. The quantification of mutagenic events on pollen grains also were measured. For this, median leaves and pre-anthesis flowers were collected from the adult plants from three places of Curitiba and one place in Araucaria, all nearby to the air monitoring stations. The comparison of the four study sites showed a reduction in leaf area, an increasing of stomatal density, subepidermic layer, epidermis in both faces and the amount of micronucleus. Also, there was reduction of chlorophyllian parenchymas at the site where there was the higher average for the ozone level. It was concluded... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Air quality; Tropospheric pollution; Bioindication; Vegetal morphoanatomy; Morphometry; Mutagenesis. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400020 |
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| Massironi,S.M.G.; Reis,B.L.F.S.; Carneiro,J.G.; Barbosa,L.B.S.; Ariza,C.B.; Santos,G.C.; Guénet,J.L.; Godard,A.L.B.. |
| When compared to other model organisms whose genome is sequenced, the number of mutations identified in the mouse appears extremely reduced and this situation seriously hampers our understanding of mammalian gene function(s). Another important consequence of this shortage is that a majority of human genetic diseases still await an animal model. To improve the situation, two strategies are currently used: the first makes use of embryonic stem cells, in which one can induce knockout mutations almost at will; the second consists of a genome-wide random chemical mutagenesis, followed by screening for mutant phenotypes and subsequent identification of the genetic alteration(s). Several projects are now in progress making use of one or the other of these... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mouse; Ethylnitrosourea; Mutation; Co-isogenic mutations; Mutagenesis. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000900009 |
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| Rosa,R.M.; Roesler,R.; Braga,A.L.; Saffi,J.; Henriques,J.A.P.. |
| The pharmacology of synthetic organoselenium compounds indicates that they can be used as antioxidants, enzyme inhibitors, neuroprotectors, anti-tumor and anti-infectious agents, and immunomodulators. In this review, we focus on the effects of diphenyl diselenide (DPDS) in various biological model organisms. DPDS possesses antioxidant activity, confirmed in several in vitro and in vivo systems, and thus has a protective effect against hepatic, renal and gastric injuries, in addition to its neuroprotective activity. The activity of the compound on the central nervous system has been studied since DPDS has lipophilic characteristics, increasing adenylyl cyclase activity and inhibiting glutamate and MK-801 binding to rat synaptic membranes. Systemic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diphenyl diselenide; Organoselenium; Antioxidants; Neuroprotection; Saccharomyces cerevisiae; Mutagenesis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000001 |
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| Kanan,Ghassan J. M.; Al-Najjar,Heyam E.. |
| We aimed her mainly to evaluate the contribution of newly employed bromate selection system, in obtaining new Aspergillus niger nitrate/nitrite assimilation defective mutants, through Ultraviolet treatment (UV), 1, 2, 7, 8-Diepoxyoctane (DEO), phenols mixture (Phx)) and spontaneous treatments. The newly employed bromate selection system was able to specify only two putative novel mutant types designated brn (bromate resistant but chlorate sensitive (RS) strain, which may specify nitrite specific transporter) and cbrn mutants (bromate resistant and chlorate resistant strain, which may specify nitrate/nitrite bispecific system). The most relevant and innovative findings of this research work involve the isolation of the RR (cbrn) mutants (a new type of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspergillus niger; Chlorate; Bromate; Mutagenesis. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400032 |
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| Berenschot,Amanda S.; Zucchi,Maria I.; Tulmann-Neto,Augusto; Quecini,Vera. |
| Traditionally, mutagenesis has been used to introduce novel genetic variability in ornamental crops. More recently, it has become a powerful tool in gene discovery and functional analyses in reverse genetics approaches. The present work aimed to compare the efficiency of physical and chemical agents in generating mutant populations of petunia. We have indirectly evaluated the genomic damage by analyzing developmental characteristics of the plantlets derived from treated seeds employing gamma radiation at 0, 20, 40, 60, 80 and 100 Gy and the alkylating agent ethyl-methanesulfonate (EMS) at 0, 0.05, 0.1, 0.15, 0.2 and 0.25% (v/v). Gamma rays and EMS caused developmental defects and decreased seedling viability in plants obtained from the mutagenized seeds.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: EMS; Gamma irradiation; Genomic damage; Mutagenesis; Reverse genetics. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202008000200002 |
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| Düsman,Elisângela; Ferreira,Márcia Flores da Silva; Berti,Alessandra Paim; Mariucci,Rosinete Gonçalves; Mantovani,Mário Sérgio; Vicentini,Veronica Elisa Pimenta. |
| Fruits are important sources of nutrients in human diet, and Barbados Cherry (Malpighia glabra L.) is of particular interest due to its high content of antioxidants. Diets rich in fruits and vegetables protect individuals against diseases and cancer, but excessive intake of vitamins may act as pro-oxidant and generate changes in DNA. To evaluate the effect of different in natura (BAN) and frozen (BAF) Barbados Cherry pulp concentrations and synthetic vitamin C in liquid form (VC) on the chromosome level and the cell cycle division, root meristeme cells of Allium cepa L. and bone marrow cells of Wistar rats Rattus norvegicus, were used as test system. In Allium cepa L., BAN, at the highest concentration (0.4 mg.mL-1) and BAF, at the lowest concentration... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidants; Cytotoxicity; Mutagenesis. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000200030 |
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| Zhang,Yu; Li,Zhen-Hua; Zheng,Wei; Tang,Zhen-Xing; Zhang,Zhi-Liang; Shi,Lu-E. |
| Background: To identify the critical amino acid residues that contribute to the high enzyme activity and good thermostability of Yersinia enterocolitica subsp. palearctica (Y. NSN), 15 mutants of Y. NSN were obtained by site-directed mutagenesis in this study. And their enzyme activity and thermostability were assayed. Effect of several factors on the enzyme activity and thermostability of Y. NSN, was also investigated. Results: The results showed that the I203F and D264E mutants retained approximately 75% and 70% enzyme activity, respectively, compared to the wild-type enzyme. In addition to the I203F and D264E mutants, the mutant E202A had an obvious influence on the thermostability of Y. NSN. According to the analysis of enzyme activity and... |
| Tipo: Journal article |
Palavras-chave: Factors affecting enzyme activity; Nuclease; Mutation; Mutagenesis; Nucleases without sequence specificity. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600005 |
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| Besoain,Ximena A; Pérez,Luz María; Araya,Antonio; Lefever,Llacolén; Sanguinetti,; Montealegre,Jaime R. |
| The obtainment of 30 new strains from native Trichoderma harzianum after UV light irradiation (UV-A and UV-C), and of 82 strains resulted from protoplast fusion were accomplished. The new strains, initially selected for their growing rate under low temperature and high pH conditions, as well as for their innocuousness on tomato plants, were tested for in vitro inhibition of Pyrenochaeta lycopersici in dual cultures and due to secretion of volatile and diffusible metabolites. All the UV-A and UV-C selected candidate mutants were innocuous to tomato plants, but none of them showed improvement in their biocontrol activity on P. lycopersici. Th12A20.1 increased 1.3 and 1.9 fold the total fresh weight of Fortaleza tomato plants when compared to its parental... |
| Tipo: Journal article |
Palavras-chave: Corky root; Mutagenesis; Tomato diseases; Trichoderma; Volatile and diffusible metabolites. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400013 |
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| Registros recuperados: 32 | |
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