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Multi-residue method of pesticides by UPLC-MS/MS in bivalve mollusks samples as a tool for food quality and safety. Repositório Alice
CASTRO, I. M. de; BASTOS, L. H. P.; ANJOS, M. R. dos; OLIVEIRA, A. C. DE; COSTA, D. M. S. F. DA; CARDOSO, M. H. W. M.; CARVALHO, L. M. J. DE.
Bivalve molluscs (BM) are filter animals and bioaccumulators of substances from the environment. This characteristic allows a great absorption of nutrients that makes them a source of protein-rich foods. On the other hand, if there are toxic contaminants in the environment, their absorption by animals may occur. This happens with pesticides coming from agricultural and livestock production systems that can migrate to areas of BM crops. Considering the high nutritional value of bivalve molluscs and their positive impact on the human diet, these products must be carefully evaluated for the possible presence of toxic substances in order to guarantee their safety. Thus, the aim of this work was to implement and validate a multi-residue method using tandem mass...
Tipo: Artigo de periódico Palavras-chave: Mollusk bivalves; UPLC MS MS; Oyster; Scallop; Pesticides.
Ano: 2023 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1154923
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Growth and antioxidant response of juvenile oysters Crassostrea sikamea and Crassostrea corteziensis treated with Streptomyces strains Arq. Bras. Med. Vet. Zootec.
García-Bernal,M.; Medina-Marrero,R.; Campa-Córdova,Á.I.; Mazón-Suástegui,J.M..
ABSTRACT The effect of three Streptomyces strains (N7, RL8 and V4) and a mixture of Bacillus (BMix) on the growth (Weight, Size) and superoxide dismutase activity (SOD) in hatchery-reared juvenile oysters Crassostrea corteziensis and Crassostrea sikamea was investigated to determine their probiotic potential. Microorganisms were added to culture water at 1×106 CFU/ml once a day during 30 days and all oysters fed daily a microalgae mix. Juveniles of C. sikamea treated with strains N7, RL8 and V4 had a significant weight gain compared to the control group. C. corteziensis juveniles treated with strains RL8 and BMix showed a significantly higher weight gain than the control group. No significant size increase was observed in any treated group for both oyster...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Actinomycetes; Oyster; Growth; Probiotic effect; SOD activity.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000601993
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Relationship between environmental variables and growth of Pteria colymbus (Mollusca Bivalvia: Pteriidae), at three cultivation depths Boletín de Investigaciones
Freites,Luis Felipe; Guevara,Miguel; Márquez,Adrián; Revilla,Jormil; Narváez,Mariela; Lodeiros,César.
ABSTRACT We studied the influence of environmental variables associated with three water depths (2, 6 and 10 m) on shell growth, soft tissues dry mass (STDM) and survival of the Caribbean winged oyster Pteria colymbus (Roding, 1798). chlorophyll-a (Chl-a), dissolved oxygen, particulate organic matter (POM) and temperature were studied at each depth. Oysters were placed in 135 plastic baskets with five individuals in each basket. Three baskets at each depth were chosen at monthly intervals for measurements of biometric parameters, biofouling, and survival. At the end of the study, dry mass of the individuals grown at 10 m was significantly lower (Kruskal-Wallis non-parametric test KW, P < 0.05) with the highest values of STDM corresponding to oysters...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofouling; Environment; Depth; Oyster; Survival.
Ano: 2020 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97612020000100009
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Raw oysters can be a risk for infections BJID
Vieira,Regine Helena Silva dos Fernandes; Sousa,Oscarina Viana de; Costa,Renata Albuquerque; Theophilo,Grace Nazareth Diogo; Macrae,Andrew; Fonteles Filho,Antonio Adauto; Rodrigues,Dália dos Prazeres.
The aims of this study were to count and identify sucrose positive and negative vibrios isolated from cultivated Crassostrea rhizophorae oysters during their growing cycle. Every month for 12 months, 10 to 18 oysters were collected for study. Collections occurred at the Center for Studies of Coastal Aquaculture (CSCA), which is associated with the Institute of Marine Science, Labomar, located in Euzebio, Ceará, Brazil. Approximately 150 oysters and their intervalvular liquor were studied. Vibrio Standard Plates Counts (SPC) from oyster meat and their intervalvular liquor varied from 25 to 59,000,000 CFU/g. For most of the 12 months of the oysters' life, it was possible to identify Vibrio parahaemolyticus. Vibrio carchariae was identified in four...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vibrio spp; Oyster; Public health; Vibrio parahaemolyticus; Vibrio carchariae.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100013
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Bacteria of fecal origin in mangrove oysters (Crassostrea rhizophorae) in the Cocó River estuary, Ceará State, Brazil BJM
Silva,Ana I.M.; Vieira,Regine H.S.F.; Menezes,Francisca G.R.; Fonteles-Filho,Antonio A.; Torres,Regina C.O.; Sant'Anna,Ernani S..
This study was aimed at evaluating the microbiological quality of mangrove oysters (Crassostrea rhizophorae), collected at a natural oyster bed in the estuary of Cocó river (Fortaleza, Ceará, Brazil). MPN values were used for estimating the total (TC) and fecal (FC) coliforms and Enterococcus spp. TC and FC MPN values in the whole muscle and intervalve liquid ranged from <1.8 to >1,600/g and from <1.8 to 920/g, respectively. The MPN estimates for Enterococcus spp. were between <3.0 and >1,100/g. No correlation was found between the physico-chemical parameters (temperature, salinity and pH) of the surrounding water and the bacteriological contamination levels found in the tested oysters. The only correlation found was between TC and FC...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oyster; Total and fecal coliforms; Enterococci; Bacteriocins.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100021
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Энергетический баланс и чистая эффективность роста плоской устрицы (Ostrea edulis L.) IBSS Repository
Золотницкий, А. П.; Сытник, Н. А..
Исследованы энергетический баланс и чистая эффективность роста (коэффициент К2) плоской устрицы (Ostrea edulis L.) в онтогенезе. Выявлены общие тенденции изменений отдельных его компонентов в процессе роста и распределение энергетических трат на индивидуальную продукцию, энергетический обмен, ассимилированную и потреблённую пищу. Дана характеристика сезонных изменений чистой эффективности роста, связанных с температурой воды и массой тела, динамика которых описывается уравнением множественной регрессии. Показано, что максимальные значения К2 у исследованного вида могут составлять 0.63 – 0.67.
Tipo: Journal Contribution Palavras-chave: Устрица; Энергетический баланс; Рост; Метаболизм; Ассимиляция; Рацион; Эффективность; Устриця; Енергетичний баланс; Зростання; Метаболізм; Асиміляція; Раціон; Ефективність; Oyster; Energy balance; Growth; Metabolism; Assimilation; Ration; Efficiency.
Ano: 2010 URL: http://repository.ibss.org.ua/dspace/handle/99011/1919
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Diagnostic moléculaire de l'origine des contaminations fécales dans l'environnement littoral - Développement de marqueurs Bacteroidales spécifiques de l'hôte ArchiMer
Mieszkin, Sophie.
Human and animal faecal pollution affects environmental water in inland and coastal areas, with negative implications for recreational uses, public safety and shellfish sanitary status due to the presence of enteric pathogens. Starting from 2011, the revised Bathing Water European Directive (2006/7/CE) requires the establishment of bathing water profiles with an inventory and study of the pollution sources likely to affect water quality. The faecal microbiological indicators used in these regulations, Escherichia coli and enteroccoci, cannot distinguish between human and animal faecal contamination. Thus, alternative methods, that focus on target microorganisms such as bacteria belonging to the Bacteroidales order were developed or are being developed to...
Tipo: Text Palavras-chave: Contaminations fécales humaines; Porcines et ruminants; Bacteroidales; Gènes codant les ARNr 16S; PCR quantitative en temps réel; Eaux; Persistance; Huîtres; Human; Porcine and ruminant faecal contaminations; Bacteroidales; 16S rRNA genes; Quantitative real-time PCR; Water; Persistence; Oyster.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00021/13206/10258.pdf
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Epidémiologie de Bonamia ostreae et Marteilia refringens en Bretagne en 1984 et 1985 : situation et évolution ArchiMer
Tige, Gilbert; De Kergariou, Gabriel; Cochennec, Nathalie; Rabouin, Marie-agnès.
A systematic surveillance progam conducted since 1980 along the coasts of Britanny has followed the evolution and epidemiological state of the natural O. eulis oyster beds and seed areas in the intertidal zones and deep water. Presently, Marteilia refringens exists endemically in Brittany estuaries ; the rate and degree of infestation varies from year to year depending on the hydrological conditions and the geographical region. In no case, however, is the return of flat oyster culture envisoned. Bonamia ostreae is present not only in the estuaries but also in the deep-water rearing areas (Baie de Quiberon, Cancale, Baie de St Brieuc). The infestation increases with age, but also depends on the quantity of the stock and the culture practises. In particular,...
Tipo: Text Palavras-chave: Huître plate; Ostrea edulis; Maladie; Bonamia ostreae; Marteilia refringens; Bretagne; Oyster; Ostrea edulis; Diseases; Bonamia ostreae; Marteilia refringens.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/00022/13299/10353.pdf
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Understanding the dynamics of δ13C and δ15N in soft tissues of the bivalve Crassostrea gigas facing environmental fluctuations in the context of Dynamic Energy Budgets (DEB) ArchiMer
Emmery, Antoine; Lefebvre, Sebastien; Alunno-bruscia, Marianne; Kooijman, S. A. L. M..
We studied the dynamics of stable isotopes δ13C and δ15N of an opportunistic suspension feeder the Pacific oyster (Crassostrea gigas) to better understand the factors that influence the trophic enrichment (trophic-shift, Δ) between primary producers and consumers. Most of the previous studies on this topic do not quantify mass fluxes or isotopic discrimination phenomena in the organism, which are two pillars in isotope ecology. We used a dynamic energy budget (DEB) approach (Kooijman, 2010) to quantify i) the fluxes of elements and isotopes in C. gigas soft tissues and ii) the impact of the scaled feeding level, the organism mass and the isotopic ratio of food on the "trophic-shift" Δ, and isotope turnover in tissues. Calibration and parametrization...
Tipo: Text Palavras-chave: Oyster; Isotopic ratio; Discrimination; Trophic-shift; Diet; DEB theory.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00043/15434/12784.pdf
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L'éthoxyrésorufine-o-déethylase, les adduits à l'ADN et les mocronuclei dans les organismes marins. Application à la surveillance des effets biologiques sur les côtes françaises ArchiMer
Burgeot, Thierry.
Three biomarkers [ethoxyresorufm-o-deethylase (EROD), DNA adducts and micronuclei (MN) were studied for biomonitoring applications along French coasts. EROD was developed at an experirnental stage in two pilot sites, Seine Bay and Fos sur Mer, using the dab (Limanda limanda), dragon et (Callionymus lyra), red mullet (Mullus barbatus) and comber (Serranus hepatus) as target species. Higbest EROD activity was observed in the subestuarial part of Seine Bay and at the stations closest to sources of pollution from Cortiou and Fos sur Mer. The suitable number of fish for detection of a significant difference between two EROD activities was deterrnined as a function of sampling variance and a preselected statistical power. A preliminary study on hepatocytes in a...
Tipo: Text Palavras-chave: Ethoxyrésorufine-o-déethylase; Adduits à l'ADN; Micronuclei; Poisson; Moule; Huître; Biosurveillance; Ethoryresorufin-o-deethylase; DNA adducts; Micronuclei; Fish; Mussel; Oyster; Biomonitoring.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00443/55462/56984.pdf
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Report of the workshop on shellfish bivalve cultivation, growth, modelling and impact on the ecosystem ArchiMer
Heral, Maurice; Bayne, B..
The Workshop was held in Plymouth (6-10 October 1996) in the Royal Plymouth Corinthian Yacht club organised by the Plymouth Marine Laboratory under the joint charmanships of B. Bayne et M. Héral. 36 scientists were participated to this meeting from 11 countries, 8 belonging to ICES (Annex 1) 30 communications have been presented. This meeting was supported by an European Union contrat from DG14 with a concerted action AIR3 CT 942219 on Trophic capacity of coastal zone of oysters, mussels and cockles cultivation. This contract has paid the travel cost of all the participants.
Tipo: Text Palavras-chave: Coastal systems; Carrying capacity; Physiological results; Oyster; Shefflish bivalve.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/1996/acte-7350.pdf
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Oyster reproduction is affected by exposure to polystyrene microplastics ArchiMer
Sussarellu, Rossana; Suquet, Marc; Thomas, Yoann; Lambert, Christophe; Fabioux, Caroline; Pernet, Marie Eve Julie; Le Goic, Nelly; Quillien, Virgile; Mingant, Christian; Epelboin, Yanouk; Corporeau, Charlotte; Guyomarch, Julien; Robbens, Johan; Paul-pont, Ika; Soudant, Philippe; Huvet, Arnaud.
Plastics are persistent synthetic polymers that accumulate as waste in the marine environment. Microplastic (MP) particles are derived from the breakdown of larger debris or can enter the environment as microscopic fragments. Because filter-feeder organisms ingest MP while feeding, they are likely to be impacted by MP pollution. To assess the impact of polystyrene microspheres (micro-PS) on the physiology of the Pacific oyster, adult oysters were experimentally exposed to virgin micro-PS (2 and 6 µm in diameter; 0.023 mg·L−1) for 2 mo during a reproductive cycle. Effects were investigated on ecophysiological parameters; cellular, transcriptomic, and proteomic responses; fecundity; and offspring development. Oysters preferentially ingested the 6-µm micro-PS...
Tipo: Text Palavras-chave: Microplastic; Reproduction; Energy allocation; Oyster.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00311/42233/41600.pdf
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Ingestion of a bacterivorous ciliate by the oyster Crassostrea gigas: Protozoa as a trophic link between picoplankton and benthic suspension-feeders ArchiMer
Le Gall, Solange; Bel Hassen, Malika; Le Gall, Pierre.
The linked concepts of 'microbial loop' and 'protozoan trophic link' have been very well documented in filter-feeding microzooplankton such as copepods, but have not been applied to energy transfer to benthic suspension-feeding macrofauna, with the exception of the recent demonstration of heterotrophic flagellate assimilation by mussels. The oyster Crassostrea gigas obtains energy resources by filtering microalgae (similar to 5 to 100 mu m). However, in turbid estuaries, light-limited phytoplanktonic production cannot entirely account for oyster energy requirements. Conversely, picoplankters (<2 mu m), which are main effecters of coastal energy flow and matter cycling, are not efficiently retained by oyster filtration. Ciliate protozoal as both...
Tipo: Text Palavras-chave: Oyster; Food sources; Picoplankton; Protozoa; Trophic link.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00337/44788/44482.pdf
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Etude et modélisation de la dynamique des populations de bivalves en élevage (Crassostrea gigas et Mytilus galloprovincialis) dans le bassin de Thau (Méditerranée, France) et des ascidies solitaires associées ArchiMer
Gangnery, Aline.
The total standing stock of bivalves cultured in Thau Lagoon (France) that is mainly constituted of the Pacific oyster, Crassostrea gigas, and the Mediterranean mussel, Mytilus galloprovincialis, vary between 14,000 and 20,000 tons within a year. These variations were attributed to the rearing cycle of molluscs in the lagoon but also to their growth performances. Oysters cultured in the Thau Lagoon presented the best growth rate ever reported whereas trophic conditions are low : mean concentrations in organic particulate matter and total chlorophyll a averaged 1 mg.l-1 and 1.2 µg.l-1, respectively. The development of a growth model shows that temporal pattern (i.e. seasonality) of bivalve growth is explained by water temperature and spatial pattern is...
Tipo: Text Palavras-chave: Lagoon ecosystem; Resource management; Rearing strategies; Production; Standing stock; Hydrobiology; Growth; Shellfish culture; Ascidiella aspersa; Mussel; Oyster; Ecosystème lagunaire; Gestion des ressources; Stratégies d'élevage; Production; Stock; Hydrobiologie; Croissance; Conchyliculture; Ascidiella aspersa; Moule; Huître.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/these-102.pdf
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Les risques conchylicoles en Baie de Quiberon. Deuxième partie : le risque de prédation sur l’huître creuse Crassostrea gigas. Rapport final du projet Risco 2010-2013 ArchiMer
Mazurie, Joseph; Stanisiere, Jean-yves; Bouget, Jean-francois; Langlade, Aime; Leclerc, Emilie; Quinsat, Kevin; Herve, Gilles; Augustin, Jean-marie; Ehrhold, Axel; Sinquin, Jean-marc; Meidi-deviarni, Ika; Goubert, Evelyne; Cochet, Helene; Dreano, Alain.
This study (“Risco”), implicating both industry, socio-economic experts and biologists, was funded by the Regional Council of Brittany, for 3 years (2010-2012), to investigate about the causes of oysters (Crassostrea gigas) mortalities in the bay of Quiberon (South Brittany, France). The protocol including both a sonar and video visualization of the bottom, and a monthly survey of 15 experimental oyster structures, allowed to assess the risk due to predation. A clear spatial distribution of the predatory effect was evidenced, with starfish (Asterias rubens and Marthasterias glacialis) predominating in the North-East sector, and boring snails (Ocenebra erinacea and Pterophora inornata) in the South-Center area.. The main mortalities from these predators...
Tipo: Text Palavras-chave: Baie de Quiberon; Huître; Crassostrea gigas; Risque; Mortalité; Prédation; Étoiles de mer; Bigorneaux perceurs; Risco; Bay of Quiberon; Oyster; Crassostrea gigas; Risk; Mortality; Predation; Starfish; Oyster drills; Risco.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00180/29130/27534.pdf
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Déterminismes génétiques de l'allocation à la reproduction chez les huîtres creuses (Crassostrea gigas) triploïdes ArchiMer
Normand, Julien.
In diploid Pacific oyster Crassostrea gigas, gonadic development can be important in terms of energetic investment. Phenotypic and genetic negative correlations have also been observed between gonadic occupation and traits related to survival and growth, and this fact suggests that this trait could be deleterious for its aquaculture production. Triploidy can be been artificially induced to improve the production of Pacific oysters as it results in a general diminution of mean gonadic development. This trait is however highly variable. In this context, this study aimed to quantify and qualify the allocation to reproduction in triploid oysters and to study its genetic bases. Variability for allocation to reproduction and growth was first studied in 3 sets of...
Tipo: Text Palavras-chave: Triploidy x genetic interaction; Genetic determinism; Sexual dimorphism; Gametogenesis; Allocation to reproduction; Triploid; Oyster; Interaction triploïdie x génétique; Déterminisme génétique; Dimorphisme sexuel; Gamétogenèse; Allocation à la reproduction; Triploïde; Huître.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/these-6971.PDF
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Effects of Alexandrium minutum exposure on nutrition-related processes and reproductive output in oysters Crassostrea gigas ArchiMer
Haberkorn, Hansy; Lambert, Christophe; Le Goic, Nelly; Moal, Jeanne; Suquet, Marc; Gueguen, Marielle; Sunila, Inke; Soudant, Philippe.
This study assessed the effects of an artificial bloom of the toxin-producing dinoflagellate, Alexandrium minutum, upon nutrition related processes and reproductive output of the Pacific oyster, Crassostrea gigas. Oysters were exposed to A. minutum, Paralytic Shellfish Toxins (PST) producer and compared to a control batch of oysters fed Isochrysis galbana clone Tahitian (T.Iso). The experiment was performed in June 2008, when oysters were found ripe. Several physiological variables of oysters, such as PSTs accumulation, digestive gland and histological observations as well as spermatozoa quality, were measured at the end of the exposure. Results indicate that the digestive gland was greatly impacted upon A. minutum exposure. Monoacylglycerol and...
Tipo: Text Palavras-chave: Harmful algae; Alexandrium minutum; Oyster; Nutrition-related processes; Reproductive output.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00008/11927/8653.pdf
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Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas ArchiMer
Bigot, Laetitia; Zatylny-gaudin, Celine; Rodet, Franck; Bernay, Benoit; Boudry, Pierre; Favrel, Pascal.
Gonadotropin-releasing hormone (GnRH), a key neuropeptide regulating reproduction in vertebrates has now been characterized in a number of non-vertebrate species. Despite the demonstration of its ancestral origin, the structure and the function of this family of peptides remain poorly known in species as distant as lophotrochozoans. In this study, two GnRH-related peptides (Cg-GnRH-a and CgGnRH-G) were characterized by mass spectrometry from extracts of the visceral ganglia of the Pacific oyster Crassostrea gigas. These peptides showed a high degree of sequence identity with GnRHs of other mollusks and annelids and to a lesser extent with those of vertebrates or with AKH and corazonins of insects. Both the mature peptides and the transcript encoding the...
Tipo: Text Palavras-chave: Mollusk; Bivalve; Oyster; Neuroendocrinology; Reproduction; GnRH.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00079/19003/17477.pdf
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Comparative study on the contamination and decontamination of Japanese oyster Crassostrea gigas and blue mussel Mytilus edulis by oxytetracycline and oxolinic acid ArchiMer
Pouliquen, Hervé; Le Bris, Hervé; Buchet, Vincent; Pinault, Louis.
Marine fish farms use many antibacterial agents to prevent or fight bacterial diseases. The major part of the orally supplied drugs reach the environment, either directly due to excessive feeding and reduced appetite of the cultured fish, or indirectly after passing through the fish. Some of the drugs entering the environment may be taken up by bivalves. Comparative contamination and decontamination of Japanese oyster Crassostrea gigas and blue mussel Mytilus edulis were studied when these bivalves were kept during 10 d in seawater containing oxytetracycline (OTC) or oxolinic acid (OA) at concentrations of 0.15 and 1.50 mg l(-1). After the 10 d exposure at the seawater concentrations of 0.15 and 1.50 mg l(-1), OTC and OA concentrations in bivalve tissues...
Tipo: Text Palavras-chave: Mussel; Oxolinic acid; Oxytetracycline; Oyster.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00282/39359/39437.pdf
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Detection and description of a particular Ostreid herpesvirus 1 genotype associated with massive mortality outbreaks of Pacific oysters, Crassostrea gigas, in France in 2008 ArchiMer
Segarra, Amelie; Pepin, Jean-francois; Arzul, Isabelle; Morga, Benjamin; Faury, Nicole; Renault, Tristan.
Ostreid herpesvirus 1 (OsHV-1) infections have been reported around the world and are associated with high mortalities of the Pacific oyster (Crassostrea gigas). In the summer 2008, abnormal mortality rates ranging from 80% to 100% were reported in France and affected only Pacific oysters. Analyses of oyster samples collected during mortality outbreaks demonstrated a significant detection of OsHV-1 (75% of analysed batches), which appeared stronger than previous years. DNA sequencing based on C and IA regions was carried out on 28 batches of OsHV-1 infected Pacific oysters collected in 2008. Polymorphisms were described in both the C and IA regions and characterized a genotype of OsHV-1 not already reported and termed OsHV-1 μVar. A microsatellite zone...
Tipo: Text Palavras-chave: Herpes virus; Oyster; Crassostrea gigas; OsHV-1 mu Var; Increased mortality; Microsatellites.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00011/12260/9035.pdf
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