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| Registros recuperados: 27 | |
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| Souza,Marco A. A.; Barbosa,Verônica S.; Albuquerque,Jones O.; Bocanegra,Silvana; Souza-Santos,Reinaldo; Paredes,Helen; Barbosa,Constança S.. |
| Realizou-se levantamento malacológico na praia de Carne de Vaca, município de Goiana, litoral norte de Pernambuco, entre novembro de 2006 e outubro de 2007, com o objetivo de conhecer a fauna malacológica dessa localidade e verificar as condições naturais, pouco ou bastante alteradas das áreas de estudo através da aplicação de um protocolo de avaliação de diversidade de hábitats. Foram coletados 5.912 moluscos, representados por sete espécies e quatro famílias, dos quais, 5.209 exemplares de Biomphalaria glabrata (Say, 1818), 113 de Drepanotrema lucidum (Pfeiffer, 1839), 55 de Drepanotrema cimex (Moricand, 1837), 13 de Drepanotrema anatinum (Pfeiffer, 1839), 222 de Melanoides tuberculatus (Muller, 1774), 263 de Pomacea sp. e 37 de Physa marmorata Guilding,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gastropoda; Schistosoma mansoni; Praia de Carne de Vaca; Ambiente de água doce. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212010000100003 |
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| De Carvalho Augusto, Ronaldo; Rey, Oliver; Cosseau, Céline; Chaparro, Cristian; Vidal-dupiol, Jeremie; Allienne, Jean-françois; Duval, David; Pinaud, Silvain; Tönges, Sina; Andriantsoa, Ranja; Luquet, Emilien; Aubret, Fabien; Dia Sow, Mamadou; David, Patrice; Thomson, Vicki; Joly, Dominique; Gomes Lima, Mariana; Federico, Déborah; Danchin, Etienne; Minoda, Aki; Grunau, Christoph. |
| We describe here a protocol for the generation of sequence-ready libraries for population epigenomics studies, and the analysis of alignment results. We show that the protocol can be used to monitor chromatin structure changes in populations when exposed to environmental cues. The protocol is a streamlined version of the Assay for transposase accessible chromatin with high-throughput sequencing (ATAC-seq) that provides a positive display of accessible, presumably euchromatic regions. The protocol is straightforward and can be used with small individuals such as daphnia and schistosome worms, and probably many other biological samples of comparable size (~10,000 cells), and it requires little molecular biology handling expertise. |
| Tipo: Text |
Palavras-chave: Epigenetics; Epigenomics; ATAC-seq; Daphnia pulex; Schistosoma mansoni. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00681/79353/81859.pdf |
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| Geyer, Kathrin K.; Munshi, Sabrina E.; Vickers, Martin; Squance, Michael; Wilkinson, Toby J.; Berrar, Daniel; Chaparro, Cristian; Swain, Martin T.; Hoffmann, Karl F.. |
| Uncontrolled host immunological reactions directed against tissue-trapped eggs precipitate a potentially lethal, pathological cascade responsible for schistosomiasis. Blocking schistosome egg production, therefore, presents a strategy for simultaneously reducing immunopathology as well as limiting disease transmission in endemic or emerging areas. We recently demonstrated that the ribonucleoside analogue 5-azacytidine (5-AzaC) inhibited Schistosoma mansoni oviposition, egg maturation and ovarian development. While these anti-fecundity effects were associated with a loss of DNA methylation, other molecular processes affected by 5-AzaC were not examined at the time. By comparing the transcriptomes of 5-AzaC-treated females to controls, we provide evidence... |
| Tipo: Text |
Palavras-chave: Schistosoma mansoni; Epigenetics; 5-Azacytidine; Fecundity; RNA-Seq; Protein synthesis; Stem cells. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00615/72703/71747.pdf |
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| Picard, Marion A. L.; Vicoso, Beatriz; Roquis, David; Bulla, Ingo; Augusto, Ronaldo C.; Arancibia, Nathalie; Grunau, Christoph; Boissier, Jerome; Cosseau, Celine. |
| Differentiated sex chromosomes are accompanied by a difference in gene dose between X/Z-specific and autosomal genes. At the transcriptomic level, these sex-linked genes can lead to expression imbalance, or gene dosage can be compensated by epigenetic mechanisms and results into expression level equalization. Schistosoma mansoni has been previously described as a ZW species (i.e., female heterogamety, in opposition to XY male heterogametic species) with a partial dosage compensation, but underlying mechanisms are still unexplored. Here, we combine transcriptomic (RNA-Seq) and epigenetic data (ChIP-Seq against H3K4me3, H3K27me3, and H4K20me1 histone marks) in free larval cercariae and intravertebrate parasitic stages. For the first time, we describe... |
| Tipo: Text |
Palavras-chave: Dosage compensation; Chromatin landscape; Histone modifications; Female heterogamety; Schistosoma mansoni. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00514/62580/66929.pdf |
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| Coustau, C.; Gourbal, B.; Duval, D.; Yoshino, T. P.; Adema, C. M.; Mitta, Guillaume. |
| This review summarizes the research progress made over the past decade in the field of gastropod immunity resulting from investigations of the interaction between the snail Biomphalaria glabrata and its trematode parasites. A combination of integrated approaches, including cellular, genetic and comparative molecular and proteomic approaches have revealed novel molecular components involved in mediating Biomphalaria immune responses that provide insights into the nature of host-parasite compatibility and the mechanisms involved in parasite recognition and killing. The current overview emphasizes that the interaction between B. glabrata and its trematode parasites involves a complex molecular crosstalk between numerous antigens, immune receptors, effectors... |
| Tipo: Text |
Palavras-chave: Biomphalaria glabrata; Schistosoma mansoni; Echinostoma caproni; Immunity; Compatibility. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00276/38718/38622.pdf |
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| Angora, Etienne K.; Boissier, Jérôme; Menan, Hervé; Rey, Olivier; Tuo, Karim; Touré, Andre O.; Coulibaly, Jean T.; Méité, Aboulaye; Raso, Giovanna; N’goran, Eliézer K.; Utzinger, Jürg; Balmer, Oliver. |
| Schistosomiasis is a parasitic disease affecting more than 250 million people, primarily in sub-Saharan Africa. In Côte d’Ivoire both Schistosoma haematobium (causing urogenital schistosomiasis) and Schistosoma mansoni (causing intestinal schistosomiasis) co-exist. This study aimed to determine the prevalence of S. haematobium and S. mansoni and to identify risk factors among schoolchildren in the western and southern parts of Côte d’Ivoire. From January to April 2018, a cross-sectional study was carried out including 1187 schoolchildren aged 5–14 years. Urine samples were examined by a filtration method to identify and count S. haematobium eggs, while stool samples were subjected to duplicate Kato-Katz thick smears to quantify eggs of S. mansoni and... |
| Tipo: Text |
Palavras-chave: Côte d’Ivoire; Prevalence; Risk factors; Schistosoma haematobium; Schistosoma mansoni; Schistosomiasis. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00508/61953/66043.pdf |
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| De Carvalho Augusto, Ronaldo; Merad, Nadjiya; Rognon, Anne; Gourbal, Benjamin; Bertrand, Cédric; Djabou, Nassim; Duval, David. |
| Background Freshwater snails are the intermediate hosts of a large variety of trematode flukes such as Schistosoma mansoni responsible for one of the most important parasitic diseases caused by helminths, affecting 67 million people worldwide. Recently, the WHO Global Vector Control Response 2017–2030 (GVCR) programme reinforced its message for safer molluscicides as part of required strategies to strengthen vector control worldwide. Here, we present the essential oil from Eryngium triquetrum as a powerful product with molluscicide and parasiticide effect against S. mansoni and the snail intermediate host Biomphalaria glabrata. Methods In the present study, we describe using several experimental approaches, the chemical composition of E. triquetrum... |
| Tipo: Text |
Palavras-chave: Biomphalaria glabrata; Eryngium triquetrum; Molluscicide; Oil; Parasiticide; Schistosoma mansoni. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00652/76382/77391.pdf |
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| Fneich, Sara; Theron, Andre; Cosseau, Celine; Rognon, Anne; Aliaga, Benoit; Buard, Jerome; Duval, David; Arancibia, Nathalie; Boissier, Jerome; Roquis, David; Mitta, Guillaume; Grunau, Christoph. |
| Background: Adaptive evolution is not possible without the generation of phenotypic variants. The origin of these variations has been a central topic in evolutionary biology. Up to now, it was commonly accepted that standing genetic variation is the only cause of phenotypic variants. However, epigenetic information is emerging as a complementary source of heritable phenotypic variation that contributes to evolution. The relative importance of genetics and epigenetics in generating heritable phenotypic variation is nevertheless a matter of debate. Results: We used a host-parasite system to address this question. The human blood fluke Schistosoma mansoni can adapt rapidly to new intermediate snail hosts. The interaction between parasite and mollusk is... |
| Tipo: Text |
Palavras-chave: Epigenetics; Adaptive evolution; Compatibility polymorphism; Schistosoma mansoni. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00615/72708/71730.pdf |
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| Aemero, Mulugeta; Boissier, Jerome; Climent, Deborah; Mone, Helene; Mouahid, Gabriel; Berhe, Nega; Erko, Berhanu. |
| Background: Human intestinal schistosomiasis caused by Schistosoma mansoni and urinary schistosomiasis caused by Schistosoma haematobium are endemic in Ethiopia. Although schistosomes look morphologically uniform, there is variation in infectivity, egg productivity and virulence due to variation in their genetic make. Knowing the genetic diversity and population structure of S. mansoni isolates will enable to understand and consider the possible variability in terms of infectivity, egg productivity and virulence. Methods: Between 2010 and 2011, genetic diversity and population structure of Schistosoma mansoni isolates from four endemic areas of Ethiopia was assessed using previously published 11 polymorphic microsatellite loci. Miracidia were hatched from... |
| Tipo: Text |
Palavras-chave: Schistosoma mansoni; Microsatellite; Genetic diversity; Population structure; Ethiopia. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00302/41369/71868.pdf |
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| JKAL,Neves; S,Sarinho; CML,de Melo; VRA,Pereira; MCA,de Lima; IR,Pitta; MCPA,Albuquerque; SL,Galdino. |
| Schistosomiasis is a major public health problem with 207 million people infected and more than 779 million at risk. The drug of choice for treating schistosomiasis is praziquantel (PZQ); however, it is inefficient against immature forms of schistosomes. The aim of this study was to test new imidazolidine derivatives LPSF/PT09 and LPSF/PT10 against adult Schistosoma mansoni worms. IC50, cytotoxicity, immune response and cell viability assays were also available for these imidazolidines. Different concentrations of imidazolidine, from 32 to 320 ¼M, promoted motor abnormalities in breeding and unpaired worms, and death in 24 hours at higher concentrations. Although LPSF/PT09 and LPSF/PT10 did not affect IFN-³ and IL-10 production, they induced nitric oxide... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Schistosoma mansoni; In vitro; Imidazolidines. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300007 |
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| Miyasato,PA; Ramos,CRR; Abreu,PAE; Dias,WO; Nascimento,C; Ho,PL; Kawano,T. |
| Schistosomes utilize proteinases to accomplish several activities such as tissue penetration, tissue digestion and evasion of host immune responses. Cathepsin L is a cysteine proteinase of the papain superfamily detected in their gut lumen which indicates that this enzyme contributes to the proteolysis of ingested hemoglobin. Due to the roles played in the schistosome biology, proteolytic enzymes are considered potential targets for developing and guiding antischistosomal therapies. In the present work, the cathepsin L1 cDNA coding of Schistosoma mansoni was cloned into the pAE vector that provides high-level expression of heterologous proteins in Escherichia coli. The recombinant protein was expressed as inclusion bodies, purified under denaturing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Schistosoma mansoni; Cysteine proteinase; Cathepsin L1; Vaccine; Immunogenicity. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200011 |
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| Pinto,R. Magalhães; Noronha,Dely; Almeida,Marília S. S; Katz,Naftale; Tendler,Miriam. |
| Skin to lung migration of Schistosoma mansoni Sambon, 1907 - (75 Se) - selenomethionine-labeled cercariae was tracked by tissue autoradiography on days 1, 4, 6, 8 after challenge, in rabbits immunized with a S. mansoni derived saline extract. Either in vaccinated animals or in those of the control unprimed group, the peak of skin schistosomula occurs 24hs after infection. Comparison between peaks of lungs migrating larvae showed that, in control animals, the increase of worm burden in this site, is detected on the 6th day post-infection, differing from immunized rabbits, in which this peak occurs on day 4, when skin and lungs counts are still equivalent, decreasing gradually, showing a different pattern of the S. mansoni migration and suggesting that main... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Schistosoma mansoni; Migration; Autoradiography; Immunization; Rabbits. |
| Ano: 1994 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751994000400006 |
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| Tristão,A.R.; Melo,A.L.; Vasconcelos,A.C.; Grossi,F.M.. |
| Foram estudadas 42 amostras de fígado de camundongos inoculados com cercárias do Schistosoma mansoni, obtidas 40, 60, 80 e 120 dias após a infecção e processadas rotineiramente. As lâminas obtidas foram coradas pela HE para análise qualitativa e morfométrica do número e área dos granulomas e pelo MGP para quantificação de células apoptóticas. Os animais com 40 dias de inoculação possuíam menos granulomas/lâmina (<img src="http:/img/fbpe/abmvz/v52n6/a06img01.gif" alt="a06img01.gif (532 bytes)" align="absmiddle" > ou = 11,78±4,01), com áreas pequenas (<img src="http:/img/fbpe/abmvz/v52n6/a06img01.gif" alt="a06img01.gif (532 bytes)" align="absmiddle" > ou = 52.713,88±5.244,34<FONT FACE="Symbol">m</font>m²) e as menores médias de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Schistosoma mansoni; Apoptose; Morte celular programada; Modulação da inflamação; Granuloma. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000600006 |
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| Dkhi,Mohamed A. |
| BACKGROUND: Schistosomiasis is caused by helminth parasites of the genus Schistosoma. Berberine chloride (BER), an isoquinoline alkaloid, has been used in vivo for its antiparasitic, antioxidant and hepatoprotective properties. In this study, the protective effect of BER and praziquantel has been compared for the extent of schistosomiasis-induced oxidative stress in hepatic tissue of mice. RESULTS: S. mansoni was able to induce inflammation and injury to the liver, evidenced (i) by an increase in inflammatory cellular infiltrations, dilated sinusoids and vacuolated hepatocytes, (ii) by decreased levels of alanine and aspartate aminotransferases and increased levels of alkaline phosphatase, γ-glutamyl transferase in the liver homogenate, (iii) by... |
| Tipo: Journal article |
Palavras-chave: Schistosoma mansoni; Berberine; Liver; Mice. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100008 |
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| Filgueira,Norma Arteiro; Saraiva,Camilla Maria de Alencar; Jucá,Norma Thomé; Bezerra,Matheus Filgueira; Lacerda,Claudio Moura. |
| ABSTRACT Schistosomiasis affects approximately 207 million people in 76 countries. The association between hepatocellular carcinoma and Schistosoma mansoni infection has been investigated. Studies using animal models suggest that the parasite may accelerate the oncogenic process when combined with other factors, such as hepatitis C virus infection or exposure to a carcinogen. Herein, we report a case series of six hepatocellular carcinoma patients from Northeast Brazil, with negative serology for both hepatitis B and C virus, submitted to liver transplantation, whose explant showed evidence of schistosomal liver fibrosis. Since all patients enrolled in this study were submitted to liver transplantation, we were able to access the whole explanted liver and... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Schistosoma mansoni; Hepatocellular carcinoma; Liver transplantation. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000400352 |
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| Registros recuperados: 27 | |
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