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Comparison of dietary phospholipids and neutral lipids: effects on gut, liver and pancreas histology in Atlantic cod (Gadus morha L.) larvae ArchiMer
Wold, P. -a.; Hoehne-reitan, K.; Cahu, Chantal; Zambonino, Jose-luis; Rainuzzo, J.; Kjorsvik, E..
The aim of the present study was to compare effects of dietary n-3 highly unsaturated fatty acids (HUFA) being incorporated in the phospholipid (PL) or in the neutral lipid (NL) fraction of the larval feed, on larval growth and histology of digestive organs in Atlantic cod (Gadus morhua L.) larvae. Three isoproteic and isolipidic diets, labelled according to the percentage of n-3 docosahexaenoic acid and eicosapentaenoic acid contained in NL1 or in PL1 and PL3 of the diets, were fed to cod larvae from 17 days post hatching (dph) to 45 dph. In the liver, hepatocytes and their nuclei were smaller in NL1 larvae compared with the PL larvae; the mitochondrial membrane structures were less dense and the amount of lipids observed in the liver was significantly...
Tipo: Text Palavras-chave: Stereology; Phospholipids; Nutrition; Lipid class; Larvae; Histology; Diet; Cod.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6777.pdf
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Gametogenesis, reproductive investment, and spawning behavior of the Pacific giant oyster Crassostrea gigas: evidence of an environment-dependent strategy ArchiMer
Enriquez-diaz, M.; Pouvreau, Stephane; Chavez-villalba, J.; Le Pennec, M..
The progress of gametogenesis was studied in oysters Crassostrea gigas having the same origin (Tremblade), but cultured during 1 year in two distinctive French marine areas, the Baie des Veys and Marennes-Ol,ron. We assessed seasonal changes in the reproduction cycle on the basis of stereological techniques to estimate reproductive investment and measurement of gonad evolution area by quantitative histology. From a qualitative point of view, both oyster groups presented typical reproductive stages, but showed differential timing, in particular during the sequence of spawning and duration of the re-absorption stage. Oysters in Baie des Veys had a single partial spawning in August and a re-absorption stage that extended until winter. Oysters in...
Tipo: Text Palavras-chave: Stereology; Reproductive investment; Pacific oysters; Image analysis; Gametogenesis; Crassostrea gigas.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-7300.pdf
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Effects of Preweaning Polysensorial Enrichment upon Development of the Parietal Cortical Plate of Undernourished Rats: A Stereological Study International Journal of Morphology
González,Héctor; Adaro,Luis; Hernández,Alejandro; Fernández,Víctor.
This investigation was undertaken in order to quantify the effects of early polysensorial enrichment on the development of cortical pyramids, located in the parietal cortex of rats simultaneously submitted to protein-energy undernutrition. A short period of stimulation during suckling significantly decreases the cellular density in the cortical plate (phylogenetic-ontogenetic evolutionary index). Results suggest that the cerebral cortex develops according to a sophisticated neuronal network, which exhibits a notable degree of structural specificity, regulated by genetic and environmental clues. The most obvious prediction is that environmental influences are probably transduced as a structural expression in the developing parietal cortical plate.
Tipo: Journal article Palavras-chave: Environmental enrichment; Cortical gradients; Undernutrition; Parietal cortex; Stereology.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400016
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EFFECT OF CADMIUM ON RAT MAXILLARY MOLAR JUNCTIONAL EPITHELIUM DURING LACTATION: EFECTO DEL CADMIO SOBRE EL EPITELIO DE LA ZONA DE UNIÓN MAXILO-MOLAR DE RATAS DURANTE LA LACTANCIA International Journal of Morphology
Ribas,Paulo; Lopes,Ruberval A.; Sala,Miguel A.; Ribas,Lucia M.R.; de Mattos,Maria da Glória C.; Semprini,Marisa; Watanabe,Ii-Sei; Regalo,Simone C.H..
Cadmium in the air, drinking water and food has the potential to affect the health of people, mainly those who live in highly industrialized regions. Cadmium affects placental functions, can cross the placental barrier and directly disturbs fetal development. It is also excreted into milk. The organism is particularly susceptible to cadmium exposure at perinatal period. The effect on rat maxillary molar junctional epithelium a continuous exposure to drinking water containing low level of cadmium during lactation was studied. Female rats received drinking water ad libitum containing 300 mg/l CdCl2 throughout the whole lactation. Control animals received a similar volume of water without cadmium. Lactent rats (21 day-old) were killed by lethal dose of...
Tipo: Journal article Palavras-chave: Oral mucosae; Junctional epithelium; Cadmium; Lactation; Morphometry; Stereology.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022004000400003
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Determination of Lateral Ventricle and Brain Volume in Children with Stereological Method Using MRI International Journal of Morphology
Karacan,Keziban; Kosar,Mehmet Ilkay; Çimen,Mehmet; Solak,Orhan; Sahin,Bünyamin.
Although brain development is most active during the intrauterina period of life, the processes of myelination and arborization affect the structure of the brain throughout childhood and adolescence. Brain development is also very active in the early years of a child's life, and continues to be so for approximately 15 years after gestation. Volumetric changes in the brain are effected by sex. Understanding the variability of human brain volume during development is important for the interpretation of childhood neuroimaging studies. Hence the aim of this study is to determine the effects of sex difference on brain volume (BV), lateral ventricle volume (LV) and the ratio of brain volume and lateral ventricle volumes as a percentage (RLBV%) of 90 healthy...
Tipo: Journal article Palavras-chave: Brain; Lateral ventricles; Magnetic resonance imaging; Stereology.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100035
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High Concentration of Reticular Fibers in Periurethral Region of the Prostatic Urethra from Patients with Benign Prostatic Hyperplasia as Revealed by Stereology International Journal of Morphology
Manaia,Jorge H. M; Costa,Waldemar S; Sampaio,Francisco J. B; Figueiredo,Marcelo A; Cardoso,Gilberto P; Babinski,Marcio A.
The goal of this investigation was to analyze and quantify changes of the reticular fibers in the prostatic urethra of patients with benign prostatic hyperplasia (BPH) and compare with a control group. Prostatic urethra tissue samples were obtained from ten patients (age range 65 to 79 years, mean 66) with clinical symptoms of bladder outlet obstruction who had undergone open prostatectomy. The ten control group samples (urethral tissue samples from the transitional zone) were collected from prostates obtained during autopsy of accidental death adults of less than 25 years. The Vv of the reticular fibers was determined with stereologic methods from 25 random fields per sample using the point-count method with a M-42 grid test system. The quantitative data...
Tipo: Journal article Palavras-chave: Prostatic urethra; Reticular fibers; Stereology.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300065
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Effect of Sodium Cyclamate on the Rat Fetal Exocrine Pancreas: a Karyometric and Stereological Study International Journal of Morphology
Martins,Alex Tadeu; Santos,Fabiano de Sant´Ana dos; Scannavino,Fábio Luiz Ferreira; Pires,Juliana Rico; Zuza,Elizângela Partata; Junior,João Armando Padovani; Azoubel,Reinaldo; Di Mateo,Miguel Angel Sala; Lopes,Ruberval Armando.
The cyclamate, a sweetner substance derived from N-cyclo-hexyl-sulfamic acid, is largely utilized as a non-caloric artificial edulcorant in foods and beverages as well as in the pharmaceutical industry. The objective of this study was to evaluate karyometric and stereological alterations in the rat fetal pancreas resulting from the intraperitoneal administration of sodium cyclamate. The exocrine pancreas of ten fetuses of rats were evaluated, five treated and five controls chosen at random, in which five rats that received from the 10th to 14th days of pregnancy an intraperitoneal daily injection of sodium cyclamate at 60 mg/Kg of body weight during 5 days. At the 20th day of gestation, the animals were removed and weighed, as were their placentas; the...
Tipo: Journal article Palavras-chave: Sodium cyclamate; Exocrine pancreas; Karyometry; Stereology.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300038
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MORPHOMETRICAL AND STEREOLOGICAL ANALYSIS OF THE SUPERIOR CERVICAL GANGLION OF Rattus norvegicus SUBMITTED TO CHRONIC TREATMENT WITH CORTISOL International Journal of Morphology
Cortez,C. M.; Costa,W. S.; Babinski,M. A.; Chagas,M. A..
The present work investigates the corticoid action on the growth of the superior cervical ganglion of the rat and describes the cortisol effect during early stages of development. The study was based on morphometric and stereological analysis of the perikarya. Eight rats were treated intraperitoneally with cortisol (1mg/Kg/day) during 36 days. Treatment was initiated in the 8th day after birth and was withdrawn one day before the sacrifice. There was a significant difference (<=P0,05) for the neural mean diameter between the control group (16.78 ± 1.11mm) and treated animals (15.84 ±0.99mm). The decrease of perikarya neuronal diameter was also demonstrated by stereological methods. Morphometrical findings may suggest alterations in superior cervical...
Tipo: Journal article Palavras-chave: Morphometry; Stereology; Glucocorticoid; Sympathetic ganglion; Rat.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000300006
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Morphometric Analysis of Hemicerebellar Asymmetryn with Central Vertigo Cases: A Stereological Study International Journal of Morphology
Nuket Gocmen-Mas,H; Karabekir,Selim; Yilmaz Kusbeci,Ozge; Sahin,Bunyamin; Ertekin,Tolga; Bas,Orhan; Canan Yazici,A; Senan,Sevda.
We aimed to evaluate the relevant methods of stereology to estimate hemicerebellar asymmetry according to sex in both adult right handed vertigo cases and comparing with healthy cases. The study included 14 adult control subjects and 18 patients with vertigo. The volumes of the cerebellar hemispheres were determined by MRI using the point-counting approach of stereological methods. The mean (±SD) of the right cerebellar hemispheres in the patients with vertigo were 52.49±5.42 cm3 in males, 50.11±4.02 cm3 in females. The mean (±SD) of the left cerebellar hemispheres in the patients with vertigo were 53.11±3.70 cm3 in males, 49.73±4.69 cm3 in females. There was not significant quantitative evidence detected in terms of cerebella asymmetry between sagittal...
Tipo: Journal article Palavras-chave: Cavalier principle; Stereology; Hemicerebellar asymmetry; Magnetic resonance imaging; Vertigo.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200048
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Effect of Photoperiod and Melatonin on Volume and Cellular Parameters of Testis in Pinealectomized Rats International Journal of Morphology
Akosman,Murat Sirri; Özdemir,Vural; Taskiran,Nihal; Akalan,Mehmet Aydin; Bülbül,Aziz.
Mammalian reproductive axis is regulated by the combination of three fundamental tissues of neuroendocrine system including hypothalamus, hypophysis and gonads. In recent years, pineal gland has been included in this axis. The aim of the present study was to investigate the effect of 12L (Light):12D (Dark) photoperiod and melatonin administration (0.5 mg/kg/day; subcutaneously) on testicular volume and cellular parameters of testis at the pinealectomized (PE) rats. For this aim, twelve adult rats were firstly pinealectomized and then divided into two groups as GI and GII randomly. The GI rats served as control group and received only normal saline, whereas GII rats were the melatonin administered group. It was found that the total testicular volume,...
Tipo: Journal article Palavras-chave: Cavalieri Method; Melatonin; Optical Fractionator; Pinealectomy; Stereology; Testis.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300046
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ENALAPRIL AND VERAPAMIL ATTENUATE THE AORTIC WALL REMODELING IN NITRIC OXIDE DEFICIENCY International Journal of Morphology
Meirelles Pereira,Leila Maria; Gonçalves Bezerra,Daniele; Mandarim-de-Lacerda,Carlos Alberto.
Forty mature male rats (Wistar strain) were maintained alive for 40 days, separated in four groups of five rats each (Control, L-NAME, L-NAME + Enalapril, L-NAME + Verapamil). Three aortic wall parameters were analyzed, as follows: the Q A(SMN) (the number of the tunica media smooth muscle nuclear profiles per area), the S V(lamellae) (surface density of the aortic lamellae), and the aortic wall thickness (AWT). Rats of the L-NAME group showed high blood pressure and cardiac hypertrophy. The AWT, the Q A(SMN) and the S V(lamellae) showed the same tendency among the groups. The increased AWT, or the reduced Q A(SMN) and the S V(lamellae) in l-NAME rats were not completely prevented by the administration of enalapril or verapamil. Hypertension causes the AWT...
Tipo: Journal article Palavras-chave: Nitric oxide; Aorta; Enalapril; Verapamil; Stereology.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000100002
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Effect of Sodium Cyclamate on the Rat Fetal Liver: A Karyometric and Stereological Study International Journal of Morphology
Martins,Alex Tadeu; Azoubel,Reinaldo; Lopes,Ruberval A.; di Matteo,Miguel A. Sala; de Arruda,José Germano Ferraz.
The cyclamate, a sweetner substance derived from N-cyclo-hexyl-sulfamic acid, is largely utilized as a non-caloric artificial edulcorant in foods and beverages as well as in the pharmaceutical industry. The objective of this study was to evaluate fetal hepatic karyometric and stereological alterations in the rat fetal liver resulting from the intraperitoneal administration of sodium cyclamate. The livers of ten rats were evaluated, five treated and five controls chosen at random, in which five rats that received from the 10th to 14th days of pregnancy an intraperitoneal daily injection of sodium cyclamate at 60 mg/Kg of body weight during 5 days. At the 20th day of gestation, the animals were removed and weighed, as were their placentas, on a precision...
Tipo: Journal article Palavras-chave: Sodium cyclamate; Fetal liver; Karyometry; Stereology.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000300005
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Effects of Oral Nicotine on Rat Liver Stereology International Journal of Morphology
Valenca,Samuel Santos; Gouveia,Lucas; Alves Pimenta,Wagner; Porto,Luís Cristóvão.
Nicotine is the more abundant component in cigarette smoke. Because nicotine is first metabolized in the liver, our aim was to investígate the effects of nicotine on this organ by biochemical and stereological methods. Male Wistar rats were treated with oral nicotine (ON) diluted in drinking water during 32 days. The control group was treated with drinking water in the same period. Rats were sacrificed 24 hours after last day, the blood was collected and the liver was removed. Lipidogram was performed by enzymatic method and collagen fibers, fat globules and hepatocytes were count in the liver by stereological methods. We observed in control group preserved hepatocytes, with no presence of inflammatory cells. However in the ON group was possible to observe...
Tipo: Journal article Palavras-chave: Nicotine; Rat; Liver; Stereology.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000400037
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End-To-Side Loop Neurorrhaphy: Axonal Comparative Stereological Study International Journal of Morphology
Boer,Nagib Pezati; Viterbo,Fausto; Faleiros,Humberto Regis; Batigalia,Fernando; Boer,Ana Luísa Ricci; Carvalho Jr,Altair Antonio.
Accidents or diseases can affect the peripheral part of the nervous system, which raises clinical and surgical therapies, among others. In this context, the technique of end-to-side neurorrhaphy is a treatment option, yet its modification loop needs some additional efficacy studies. The purpose of this study was to compare, among rats, stereological results (axons volume density) after end-to-side neurorrhaphy and after end-to-side loop neurorrhaphy. Thirty Wistar rats were used, divided into six groups (five animals per group), consisting of two control groups (for the fibular and tibial nerves), two study groups for the fibular nerve (one with an end-to-side neurorrhaphy, and the other with an end-to-side loop neurorrhaphy) and two study groups for the...
Tipo: Journal article Palavras-chave: Nerve; Axon; Stereology; Neurorrhaphy.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000300032
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Beneficial Effects of Angiotensin II AT1 Blocker on Cardiovascular Adverse Remodeling Due to Nitric Oxide Synthesis Blockade International Journal of Morphology
Fernandes-Santos,Caroline; Mendonça,Leonardo de Souza; Mandarim-de-Lacerda,Carlos Alberto.
We studied with morphological tools the effects of different doses of Losartan upon the cardiovascular remodeling in nitric oxide deficient rats. At 15 weeks of age, thirty Wistar rats were separated in six groups: control (C), L-NAME (LN), and four groups were LN was given plus Losartan at different doses (1, 5, 20 and 40 mg/kg/day). The L-NAME was given for 9 weeks, the Losartan administration starting on the 2nd week of experiment. We studied the heart, thoracic aorta and superior mesenteric artery with light microscopy and stereology. The blood pressure (BP) increased since the first week of L-NAME administration, the Losartan treatment at doses of 20 and 40 mg/kg/day was efficient to reduce BP after the 7th week of treatment. The cardiac adverse...
Tipo: Journal article Palavras-chave: Losartan; Angiotensin II; L-NAME; Hypertension; Renin-angiotensin system; Stereology; L-NAME.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000400003
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Evaluation of Lumbar Vertebral Body and Disc: A Stereological Morphometric Study International Journal of Morphology
Gocmen-Mas,Nuket; Karabekir,Hamit; Ertekin,Tolga; Edizer,Mete; Canan,Yazici; Duyar,Izzet.
Anterior access to the L1-L5 vertebrae and disc spaces can be technically challenging, frequently requiring the use of an approach to a surgeon for an adequate exposure. The technique is used for lesion excision, corpectomy, vertebral body reconstruction with cages, realignment, and/or plating or screwing. For a successful anterior approach and a suitable instrumental design via screw, adequate morphometric knowledge about body of lumbar vertebrae and disc spaces and standardized volumetric data are also required for neurosurgeons. We aimed morphometric and volumetric evaluation of lumbar bodies and discs to contribute to a safe anterior approach during surgery. We evaluated vertebral body and disc morphometry using stereology in right-handed 25 adult...
Tipo: Journal article Palavras-chave: Lumbar vertebra; Body; Morphometry; Volumetry; Stereology; Anterior access.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300028
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Which Method is Gold Standard for Determination of Thyroid Volume? International Journal of Morphology
Erçikti,Nurcan; Acer,Niyazi; Apaydi,Nihal; Güven,Inanç; Zararsiz,Gökmen.
Change of the thyroid gland volume is often the symptom of most common pathological conditions some thyroid diseases. The exact calculation for the thyroid volume is very important for the assessment and management of thyroid disorders. The volume of thyroid gland, using computed tomography (CT), ultrasound (USG) and magnetic resonance imaging (MRI) has been accessed in few studies published; however a gold standard method has not yet been determined. The purpose of this study was to estimate the volume of normal thyroid gland to define an optimal correction factor therefore was to compare different techniques using the CT. We used computed tomography images obtained from 8 cadavers (2 females, 6 males) to calculate the thyroid volumes. In the present...
Tipo: Journal article Palavras-chave: Thyroid volume; Actual volume; Correction factor; Stereology.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200011
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High-Intensity Interval Training Beneficial Effects in Diet-Induced Obesity in Mice: Adipose Tissue, Liver Structure, and Pancreatic Islets International Journal of Morphology
Motta,Victor F; Aguila,Marcia B; Mandarim-de-Lacerda,Carlos A.
The study aimed to evaluate the impact of high-intensity interval training (HIIT) on adipose tissue, pancreatic islets and liver in mice fed high-fat diet. C57BL/6 male mice were fed one of two diets: standard chow (Lean group - LE) or a high-fat diet (Obese group ­ OB). After the first 12-weeks, the animals were divided into non-trained (LE-NT and OB-NT), trained groups (LE-T and OB-T), and started the exercise protocol. The HIIT protocol in the trained animals (LE-T and OB-T) compared to their counterparts (LE-NT and OB-NT) led to a reduction in size of the pancreatic islets (LE-T vs. LE-NT -40 %, OB-T vs. OB-NT -22 %) and to an increase in insulin immunodensity in pancreatic islet (LE-T vs. LE-NT +35 %, OB-T vs. OB-NT +31 %). Apart from the above...
Tipo: Journal article Palavras-chave: HIIT; Obesity; Insulin Resistance; Hepatic Steatosis; Stereology.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200042
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Kidney anatomy, histology and histometric traits associated to renosomatic index in Gymnotus inaequilabiatus (Gymnotiformes: Gymnotidae) Neotropical Ichthyology
Fernandes,Carlos Eurico; Marcondes,Sandrieley Fernanda; Galindo,Gizela Melina; Franco-Belussi,Lilian.
ABSTRACT The Gymnotus inaequilabiatus is a Neotropical fish widely distributed in marginal areas of bays. The aim of this study was to describe the main histological and histopathological traits in the head and exocrine kidney. Here, histometric and structural density techniques were associated with renosomatic index (RSI). The kidney was processed for light microscopy. Lipofuscin and hemosiderin content were visually estimated in the melonomacrophages centers (MMCs). All the biometric body variables were correlated with RSI, especially the kidney weight and gross lesions count. The general architecture of head and exocrine kidney was similar to that described for other teleost species. MMCs were prevalent in both portions and correlated with RSI in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Pathology; Renal morphology; Stereology; Teleost.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000500207
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The impact of long-term consumption of diets enriched with olive, cottonseed or sesame oils on kidney morphology: A stereological study Anais da ABC (AABC)
HASSANZADEH-TAHERI,MOHAMMADMEHDI; HASSANZADEH-TAHERI,MAHSA; JAHANI,FARNAZ; ERFANIAN,ZAHRA; MOODI,HESAM; HOSSEINI,MEHRAN.
Abstract: To date, most of studies have only focused on metabolic effects of dietary oils while recent evidence proposes that they can influence kidneys structure. Therefore, the aim of this study was to evaluate the impact of long-term consumption of olive, cottonseed and sesame oils on renal morphology in rats. 70 male Wistar rats randomly assigned into seven equal groups and treated with standard diet (control), the standard diet enriched with 10% or 20% (W/W) of either olive oil (OLI10%, OLI20%), cottonseed oil (COT10%, COT20%) or sesame oil (SES10%, SES20%) for 5 months. Quantitative features of the kidney including kidney and cortex volumes and the number of glomeruli were analyzed stereologically. Moreover, kidney sections histologically were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cottonseed oil; Histopathology; Kidney; Olive oil; Sesame oil; Stereology.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300702
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