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| Registros recuperados: 78 | |
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| Jia, Honglin; Nishikawa, Yoshifumi; Luo, Yuzi; Yamagishi, Junya; Sugimoto, Chihiro; Xuan, Xuenan. |
| The M17 family leucine aminopeptidase (LAP) hydrolyze amino acids from the N-terminus of peptides. Many LAPs from parasitic protozoa including Plasmmodium, Trypanosoma and Leishmania, have been intensely investigated because of their crucial roles in parasite biology. In this study, a functional recombinant Toxoplasma gondii LAP (rTgLAP) was expressed in Escherichia coli and its enzymatic activity against synthetic substrates for aminopeptidase, as well as the cellular localization was determined. Our results indicated that TgLAP is a functional aminopeptidase in the cytoplasma of T. gondii. |
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Palavras-chave: Toxoplasma gondii; Leucine aminopeptidase; Enzymatic activity. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2822 |
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| Pollet, Thoams; Schapira, Mathilde; Buscot, Marie-jeanne; Leterme, Sophie; Mitchell, James G; Seuront, Laurent. |
| he distribution and aminopeptidase activity of prokaryotes were investigated along a natural continuous salinity gradient in a hypersaline coastal lagoon, the Coorong, South Australia. The abundance of prokaryotes significantly increased from brackish to hypersaline waters and different sub-populations, defined by flow cytometry, were observed along the salinity gradient. While four sub-populations were found at each station, three additional ones were observed for 8.3% and 13.4%, suggesting a potential modification in the composition of the prokaryotic communities and/or a variation of their activity level along the salinity gradient. The aminopeptidase activity highly increased along the... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00174/28549/26933.pdf |
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| Santos,M.C.M.; Cerqueira,M.M.O.P.; Leite,M.O.; Souza,M.R.. |
| RESUMO Este trabalho se propôs a desenvolver um modelo preditivo para identificação de perda de estabilidade e de sedimentação em leite UAT por determinação da atividade enzimática de aminopeptidase no leite por espectrofotometria. Foram analisadas amostras de leite cru, pasteurizado e UAT após envase durante seis meses, na região Sul do Brasil. Acidez, crioscopia, gordura, extrato seco total, extrato seco desengordurado e densidade foram analisados nos leites cru e pasteurizado. Amostras de leite cru foram ainda submetidas à análise de contagem de psicrotróficos e à atividade de aminopeptidase, e amostras de leite UAT estocadas foram analisadas quanto ao grau de proteólise mediante... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leite; Qualidade; Proteólise; UAT. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000100247 |
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| Islam,Mohammed M. |
| The N-terminal amino acid sequence of an aminopeptidase from Japanese edible mushroom, Grifola frondosa, was reported to have high similarity with that of a serine proteinase from basidiomycete, Agaricus bisporous (Nishiwaki and Hayashi, 2001). The full-length cDNA and the corresponding genomic DNA of the enzyme were cloned, based on the reported N-terminal amino acid sequence. The predicted open reading frame (ORF) of the cloned cDNA, encoding a product of 379 amino acids, was expressed in E. coli using pET expression vector. The expressed pro-enzyme (40 kDa) underwent autolysis to produce the mature protein (30 kDa) and a pro-peptide (10 kDa). The mature protein and the pro-peptide remained tightly bound to each other... |
| Tipo: Journal article |
Palavras-chave: 5'-RACE; CDNA; Genomic DNA; Maitake; Refolding. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400010 |
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| SABAT,PABLO. |
| It has been postulated that both digestive capacity and intestinal biochemical features are correlated to dietary habits in birds. Therefore, it would be expected to find biochemical constraint to hydrolyze sugars in those species, which predate exclusively on marine invertebrates. In vitro intestinal activities of these enzymes were studied in Cinclodes nigrofumosus (d' Orbigny) and Cinclodes patagonicus (Gmelling). Due to differences in dietary habits between species I predicted the lack of sucrase activity in C. nigrofumosus but not in C. patagonicus. Also, low activities of maltase would be expected in both species. On the other hand due to the considerable amount of proteins and trehalose present in preys, high activities of both trehalase and |
| Tipo: Journal article |
Palavras-chave: Digestion; Dietary adaptation; Disaccharidases; Aminopeptidase-N; Cinclodes. |
| Ano: 2000 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2000000200009 |
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| Galgaro,Maria Leticia; Lopes,Catalina Romero. |
| A variabilidade genética foi avaliada dentro e entre amostras de diferentes cultivares de amendoim, Arachis hypogaea L., conhecidos como Roxo, Tatu Branco, Tatu Vermelho, Tatuí Vermelho e Tatuí (sementes com película branca), fornecidos por fazendas situadas nas regiões dos municípios de Marília, Presidente Prudente e São Manuel. Para tal análise, foi utilizada a técnica de eletroforese horizontal em gel de poliacrilamida, para os sistemas da leucil-aminopeptidase (LAP), aspartato aminotransferase (ATT) e peroxidase (PER). No sistema da leucil-aminopeptidase, foram observadas três bandas enzimáticas, denominadas LAP-A, LAP-B e LAP-C. Os padrões de bandas obtidos para o sistema da... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eletroforese; Isoenzimas; Cultivares de amendoim; Variabilidade genética. |
| Ano: 1994 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87051994000200002 |
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| Scarpassa,Vera Margarete; Tadei,Wanderli Pedro; Contel,Eucléia Primo Betioli. |
| Dados sobre a leucina aminopeptidase durante o desenvolvimento ontogenético em Anopheles nuñez-tovarìevidenciaram seis zonae de atividade, eendo a LAP1, LAP2, LAP4 e LAP5 presentes nos estádios larvais, pupae e adultos e a LAP3 e LAP6 características desses dois últimos estágios. Diferenças na intensidade de coloração foram detectadas conforme o estágio considerado. A LAP1 e LAP5 apresentam intensidade fraca em todos os estágios e a LAP2 e LAP4 mostram atividade intensa nos estádios larvais, diminuindo nos dois estágios subseqüentes. Considerando-se as funções das enzimas, foi admitido que a LAP3, no estágio de pupa, possa estar relacionada com a histólise dos tecidos larvais. Dos seis locidetectados, variação alélica foi... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 1992 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59671992000200229 |
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| SILVEIRA,Paulo Túlio de Souza; MELO,Lorena de Jesus Próculo; SOUZA,Luciane Santos; BISPO,Eliete da Silva; SOARES,Sérgio Eduardo. |
| Abstract Our objective was to characterize the protease enzymatic activity and its isoenzymes on cacao cultivars PH 16 and TSH 1188, produced in southern Bahia, linking it to the conditions of the fermentation process. Proteases were extracted and semi-purified, their activities determined changing substrate, pH and temperature, and the values compared with the parameters of fermentation (pH and temperature), and yet determined the kinetic parameters and the activity of its isoenzymes: aminopeptidase, carboxypeptidase and endoprotease. In the experimental conditions, differences in protease activities were shown as to different cocoa cultivars on various conditions. The albumin stands out as the preferred enzyme... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aminopeptidase; Carboxypeptidase; Endoprotease; Peptide bonds; Amino acids. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000300369 |
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| Ma, Hongming; Cahu, Chantal; Zambonino, Jose-luis; Yu, Hairui; Duan, Qingyuan; Le Gall, Marie-madeleine; Mai, Kangsen. |
| Large yellow croaker is an important marine fish species for aquaculture in China. Larval rearing of this species is still based on live prey feeding. The aim of this study was to describe the onset and development of the main digestive enzymes during larval development of this species, in order to obtain essential data for the formulation of a compound diet adapted to larvae. Enzymatic assays were conducted from day I post hatching to day 40 in larvae fed the following live prey sequence: rotifers, Artemia nauplii, copepods, and then frozen copepods. Assays were done on whole larvae until day 19, then on dissected larvae and on purified brush border membranes of intestine. Pancreatic enzymes, amylase, and trypsin, showed high activities from early stages,... |
| Tipo: Text |
Palavras-chave: Trypsin; Pseudosciaena crocea; Fish larval development; Amylase; Aminopeptidase N; Alkaline phosphatase. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-444.pdf |
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| Ory, Pascaline; Palesse, S.; Delmas, Daniel; Montanie, Helene. |
| The abundance and composition of microbial communities were investigated during 2007 in Marennes Oleron Bay (France) in order to characterize the biological relationships governing the planktonic food web. Compared to the results of previous years, there was a lower abundance and a lower magnitude of variations in autotrophic and heterotrophic microbial entities in 2007. Using a Spearman rank correlation analysis, a significant link for biological abundance was only noted between bacteria and ciliates. However, an interesting relationship was highlighted between chlorophyll a and bacterial aminopeptidase and beta-glucosidase exoenzymatic activities. Two characteristic periods of virus-bacteria uncoupling were observed in... |
| Tipo: Text |
Palavras-chave: Virus; Bacteria; Phytoplankton; Diversity; Aminopeptidase. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00050/16099/13571.pdf |
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| Registros recuperados: 78 | |
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