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| Registros recuperados: 153 | |
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| Linde,Giani Andrea; Luciani,Alana; Lopes,Ana Daniela; Valle,Juliana Silveira do; Colauto,Nelson Barros. |
| Abstract Basidiomycetes have several biotechnological and industrial applications such as enzyme production, bioremediation, pharmaceutical and functional food production. Due to climatic features, the preservation of several basidiomycetes is threatened, and to guarantee the preservation of this genetic resource, the development of long-term preservation techniques is necessary once there is no universal protocol for the cryopreservation of basidiomycetes. Cryopreservation is a technique in which microorganisms are submitted to ultralow temperatures. Therefore, this study aimed to collect information on the main conditions for long-term cryopreservation... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Preservation; Substrate; Fungi; Mycelial viability; Cryoprotectant. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200220 |
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| Luciano,Karina M da F.; Mata,Mario E. R. M. Cavalcanti; Fortes,Mauri; Duarte,Maria E. M.. |
| ABSTRACT Cryopreservation is a technique used to preserve genetic material at ultra-low temperatures in cryogenic germplasm banks. In order to enable the cryopreservation of a seed, it is necessary to study its appropriate freezing temperature and its water content, because seeds reach maximum physiological quality with high water contents, and they tolerate drying when they are orthodox seeds, but they may not tolerate cryopreservation. Therefore, the objective of this study was to analyze the influence of initial water content (50 to 10% wet basis) on the kinetic behavior of the lima bean seed at the temperature of −150 °C, in addition to determining... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phaseolus lunatus; Cryogenic; Modeling; Germplasm bank. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162019000300331 |
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| Riesco, Marta F.; Felix, Francisca; Matias, Domitilia; Joaquim, Sandra; Suquet, Marc; Cabrita, Elsa. |
| Sperm cryopreservation is a widely employed technique that promotes alternative techniques to contribute to broodstock management or restoration programs for species of commercial interest, endangered species or species with an interesting genotype. The preservation of genetic material from improved stocks or from the original population is extremely important for the oyster aquaculture industry to prevent the potential impacts of epidemic diseases and natural disasters. The Portuguese oyster, Crassostrea angulata, was the most important species commercialized by the shellfish industry. However, inadequate management of this industry and pathology occurrences resulted in a significant decrease in natural populations. For... |
| Tipo: Text |
Palavras-chave: Crassostrea angulata; Sperm; Cryopreservation; Motility; Viability; Fertilization. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00333/44432/44098.pdf |
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| Tsai,S.; Lin,C.. |
| Cryopreservation is a long-term storage technique to preserve the biological material without deterioration for extended period of time at least several thousands of years. The ability to preserve and store both maternal and paternal gametes provides a reliable source of fish genetic material for scientific and aquaculture purposes as well as for conservation of biodiversity. Successful cryopreservation of fish sperm have been achieved for more than 200 fish species and many fish species have been adequated for the purpose of cryobanking. Cryopreservation of fish embryo is not viable, mainly because of the same limitations as in fish oocytes, i.e., high... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryopreservation; Sperm; Embryo; Oocyte; Blastomere. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300014 |
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| Sotres-Vega,A.; Baltazares-Lipp,M.; Villalba-Caloca,J.; Gaxiola-Gaxiola,M.O.; Santibañez-Salgado,J.A.; Olmos-Zúñiga,J.R.; Jasso-Victoria,R.. |
| Cryopreservation has an immunomodulating effect on tracheal tissue as a result of class II antigen depletion due to epithelium exfoliation. However, not all epithelium is detached. We evaluated the role of apoptosis in the remaining epithelium of 30 cryopreserved tracheal grafts. Caspase-3 immunoreactivity of tracheal epithelium was studied in canine tracheal segments cryopreserved with F12K medium, with or without subsequent storage in liquid nitrogen at -196°C for 15 days. Loss of structural integrity of tracheal mixed glands was observed in all cryopreserved tracheal segments. Caspase-3 immunoreactivity in tracheal mucosa and in mixed glands was significantly decreased, in contrast to the control group and to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tracheal cryopreservation; Epithelium; Caspase-3. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200006 |
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| Zaghi Júnior,Lienine Luiz; Lopes,Ana Daniela; Cordeiro,Fábio Aparecido; Colla,Itaruã Machri; Bertéli,Míria Benetati Delgado; Valle,Juliana Silveira do; Linde,Giani Andrea; Colauto,Nelson Barros. |
| Abstract Agaricus subrufescens is a basidiomycete which is studied because of its medicinal and gastronomic importance; however, less attention has been paid to its preservation. This study aimed to evaluate the effect of sucrose addition to substrate and cryotube on the viability of Agaricus subrufescens cryopreserved at -20 °C and at -75 °C for one and two years. Zero, 10% or 20% sucrose was added to potato dextrose agar or wheat grain. The mycelia were cryopreserved in the absence of cryoprotectant or with sucrose solutions at 15%, 30% or 45%. After one or two years at -75 °C or at -20 °C, mycelia were thawed and evaluated about viability, initial time of growth, colony diameter and genomic stability. Cryopreservation... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Preservation; Freeze; Cereal grain; Monosaccharide; Agaricus blazei. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200370 |
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| Vendrame,Wagner; Faria,Ricardo Tadeu de; Sorace,Mauren; Sahyun,Sandra Aparecida. |
| Orchids are lush and highly valuable plants due to their diversity and the beauty of their flowers, which increases their commercialization. The family Orchidaceae comprises approximately 35,000 species, distributed among more than 1,000 distinct genera and 100,000 hybrids, totaling approximately 8% to 10% of all flowering plants. With the advance of agriculture and the constant destruction of their natural habitat, orchid species are collected in an indiscriminate manner by collectors and vendors, and this extractive activity threatens many species with extinction, drastically reducing their genetic variability in nature. Therefore, it is essential to seek alternatives that make the preservation of such species feasible using techniques with low... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Preservation; Orchidaceae; Ex situ conservation; Freezing tolerance; Extinction. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542014000300001 |
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| Melo-Maciel,M.A.P.; Leite-Castro,L.V.; Leite,J.S.; Oliveira,M.S.; Almeida-Monteiro,P.S.; Nunes,J.F.; Salmito-Vanderley,C.S.B.. |
| This study aimed to evaluate the extract of Aloe vera (AV) associated or not with 10% Dimethylsulfoxide (DMSO) in cryopreservation of tambaqui semen. For the formation of the pools (n= 14), 30 males were hormonally induced twice. Each pool had the objective motility, curvilinear velocity, straight-line velocity, average path velocity and morphology analyzed before and after cryopreservation of semen. The means for cryopreservation were constituted of Powder Coconut Water-104 diluent added DMSO and/or AV (5 or 10%). After cryopreservation, motility, velocities and morphology were reduced significantly when compared to fresh... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Água de coco em Pó-104; Crioprotetor; Peixe; Reprodução. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000300945 |
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| Figueiredo,Madeleine Alves de; Rosa,Sttela Dellyzete Veiga Franco da; Ricaldoni,Marcela Andreotti; Pereira,Cristiane Carvalho; Coelho,Stefânia Vilas Boas; Silva,Luciano Coutinho. |
| ABSTRACT Cryopreservation is a technique that may potentially conserve the germplasm of species of the Coffea genus for an indeterminate time. The aim of this study was to evaluate the physiological, biochemical and ultrastructural characteristics of cryopreserved seeds of Coffea arabica L., cultivar Catucaí amarelo IAC 62, which was subjected to different protocols regarding dehydration, precooling, cooling, rewarming and cathode water use. According to each protocol, the seeds were subjected to fast or slow drying to moisture contents of 17 or 20% (wet basis), cooled in different ways, and then immersed in liquid nitrogen for 24 hours. Different rewarming times in a water bath were also used. Physiological, biochemical... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cathodic water; Dehydration; Long-term conservation; Ultrafast cooling.. |
| Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542021000100203 |
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| Suquet, Marc; Dreanno, Catherine; Fauvel, Christian; Cosson, J; Billard, R. |
| Since the first work of Blaxter in 1953, fish sperm cryopreservation has been attempted on about 30 marine species. The present paper reviews the techniques used and the results published in these species. Particular attention is paid to the handling procedure of sperm before freezing, the problems of semen ageing and semen contamination with urine. The quality of frozen-thawed semen was evaluated using previously standardized biotests, such as a two-step motility activation technique adapted for the different species and fertilization assays using a discriminating insemination technique. Most extenders used in marine fish are saline or sugar solutions. From the investigated cryoprotectants, dimethyl sulphoxide (DMSO)... |
| Tipo: Text |
Palavras-chave: DMSO; Insemination; Fertilization; Cryopreservation; Fish sperm. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-611.pdf |
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| Hanquet, Anne-caroline; Kellner, K; Heude, C; Naimi, Amine; Mathieu, M; Poncet, J.m.. |
| Cryopreservation is widely used for long-term conservation of various tissues, embryos or gametes. However, few studies have described cryopreservation of invertebrate primary cell cultures and more particularly of marine invertebrate somatic cells. This technique would however be of great interest to facilitate the study of various metabolic processes which vary seasonally. The aim of the present study was to develop a protocol for cryopreservation of Crassostrea gigas vesicular cells. Different parameters were adjusted to improve recovery of cells after freezing. The most efficient cryoprotectant agent was a mix of Me2SO, glycerol, and ethylene glycol... |
| Tipo: Text |
Palavras-chave: Glycogen metabolism; Vesicular cells; Cryopreservation; Mollusc; Oyster; Crassostrea gigas. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1938.pdf |
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| ISHIJIMA, Takako; KOBAYASHI, Yoshiyasu; LEE, Dong-Soo; UETA, Yoshiko Yanagimoto; MATSUI, Motozumi; LEE, Jung-Youn; SUWA, Yoshinori; MIYAHARA, Kazuro; SUZUKI, Hiroshi; 松井, 基純; 宮原, 和郎; 鈴木, 宏志; 古林, 与志安. |
| The cryopreservation of ovarian tissues is a technology with significant potential for the preservation of the genetic resource materials of working dogs, including guide dogs for the blind. However, no attempt has been reported on cryopreservation of the canine ovary. Thus, we evaluated a vitrification method for cryopreservation of canine ovaries and determined the potential functionality of vitrified-warmed canine ovaries by means of transplantation into non-obese diabetic-severe combined immunodeficiency (NOD-SCID) mice. All ovarian tissues cryopreserved by vitrification were morphologically normal in terms of histology. Cryopreserved ovaries were... |
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Palavras-chave: Canine; Ovary transfer; Transplantation; Vitrification; Xenograft. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3023 |
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| Ishijima, Takako; Abe, Yasuyuki; Suzuki, Hiroshi; 鈴木, 宏志. |
| The effect of cryopreservation and subsequent xenotransplantation on the follicular reserve of the canine ovary by using non obese diabetic-severe combined immunodeficient (NOD-SCID) mice was examined. Vitrified-warmed canine ovarian tissues were placed into the ovarian bursa of mice, and then were removed and subjected to histological examination at 4 weeks after the transplantation. Over 30% of primordial follicles and 65% of early primary follicles survived after cryopreservation. However, regardless of breed or age, percentages of survived primordial follicles and early primary follicles after the transplantation ranged from 0-7% and from 0-15%, respectively. These results indicate... |
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Palavras-chave: Canine; Cryopreservation; Ovary; Transplantation. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2802 |
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| Demoy-schneider, Marina; Schmitt, Nelly; Le Pennec, Gael; Suquet, Marc; Cosson, Jacky. |
| High quality of sperm is essential to a high fertilization rate, especially post- cryopreservation. Assessment of sperm integrity, motility and energy reserves before cryopreservation is necessary for selection of milt with optimal fertilizing potential. We describe the effect of cryopreservation on the quality of black-lip pearl oyster, Pinctada margaritifera var. cumingii sperm. Evaluated quality indices of fresh and frozen/thawed P. margaritifera spermatozoa, included morphology, ultrastructure and motility characteristics relative to the energy content (ATP) and its capacity to be sustained by mitochondrial respiration. Morphology and ultrastructure... |
| Tipo: Text |
Palavras-chave: Spermatozoa quality; Cryopreservation; Pinctada margaritifera; Motility; Metabolism; CASA. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00453/56421/58114.pdf |
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| Registros recuperados: 153 | |
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