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| Registros recuperados: 14 | |
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| Damaso,Mônica Caramez Triches; Andrade,Carolina M.M. Carvalho; Pereira Jr,Nei. |
| In recent years, xylanases have expanded their use in many processing industries, such as pulp and paper, food and textile. Thermomyces lanuginosus IOC-4145 was able to produce a very high level of cellulase-free xylanase in shaken cultures using corncob as substrate (500 U/mL). An optimization of the medium composition in submerged fermentation was carried out aiming at a low cost medium composition for enzyme production. Statistical experiment design was employed for this purpose, pointing out corncob as the most important parameter, which affects enzyme production. Additionally, the influence of several chemicals on xylanase activity was investigated in the crude extract. A slight stimulation of the enzyme (5-15%) was achieved with NaCl and urea, both... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Xylanases; Thermomyces lanuginosus; Corncob; Submerged fermentation; Medium optimization. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400011 |
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| Wei,CB; Chen,J; Li,JH. |
| The properties and agglutination activity of acutolysin C, a hemorrhagic metalloproteinase obtained from Agkistrodon acutus venom, were studied herein. Acutolysin C is a basic glycoprotein consisting of a single polypeptide chain with a molecular weight of 23.1 kDa and pI 8.7, containing one Zn2+ and one Ca²+ per molecule. It possesses caseinolytic, weak lethal (LD50 = 7.6 mg/kg) and weak hemorrhagic (MHD = 12.0 μg) activities, but does not present fibrinolytic, fibrinogenolytic, arginine esterase and phospholipase A2 actions. In addition, it revealed agglutination activity on some animal lymphocytes, including five species of mammals, six of birds, three of reptiles and one of amphibians, but had no effect on lymphocytes from two species of reptiles, one... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agkistrodon acutus; Acutolysin C; Hemorrhagin; Leucoagglutination activity. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100005 |
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| Gururaj,P.; Ramalingam,Subramanian; Devi,Ganesan Nandhini; Gautam,Pennathur. |
| ABSTRACT The purpose of this study was to isolate, purify and optimize the production conditions of an organic solvent tolerant and thermostable lipase from Acinetobacter sp. AU07 isolated from distillery waste. The lipase production was optimized by response surface methodology, and a maximum production of 14.5 U/mL was observed at 30 ºC and pH 7, using a 0.5% (v/v) inoculum, 2% (v/v) castor oil (inducer), and agitation 150 rpm. The optimized conditions from the shake flask experiments were validated in a 3 L lab scale bioreactor, and the lipase production increased to 48 U/mL. The enzyme was purified by ammonium sulfate precipitation and ion exchange chromatography and the overall yield was 36%. SDS-PAGE indicated a molecular weight of 45 kDa for the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acinetobacter sp.; Response surface methodology; MALDI-TOF; Organic solvent tolerant lipase; Thermostable lipase. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300647 |
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| Borja-Oliveira,C. R.; Kassab,B. H.; Soares,A. M.; Toyama,M. H.; Giglio,J. R.; Marangoni,S.; Re,L.; Rodrigues-Simioni,L.. |
| Two presynaptic phospholipases A2 (PLA2), neuwieditoxin-I (NeuTX-I) and neuwieditoxin-II (NeuTX-II), were isolated from the venom of Bothrops neuwiedi pauloensis (BNP). The venom was fractionated using molecular exclusion HPLC (Protein-Pak 300SW column), followed by reverse phase HPLC (µBondapak C18 column). Tricine-SDS-PAGE in the presence or absence of dithiothreitol showed that NeuTX-I and NeuTX-II had a molecular mass of approximately 14 kDa and 28kDa, respectively. At 10µg/ml, both toxins produced complete neuromuscular blockade in indirectly stimulated chick biventer cervicis isolated preparation without inhibiting the response to acetylcholine, but NeuTX-II reduced the response to KCl by 67.0±8.0% (n=3; p<0.05).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chick biventer cervicis; Loose patch clamp; Nerve-muscle preparation; Neuromuscular junction; Neurotoxicity; PLA2 neurotoxin; Presynaptic action; Bothrops neuwiedi pauloensis; Neuwieditoxin-I; Neuwieditoxin-II. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100008 |
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| Goulart,E.C.; Pereira,C.A.T.; Garcia,R.C.; Giacomelli,M.B.O.; Rodrigues,A.L.S.. |
| Lead has been shown to produce cognitive and motor deficits in young rats that could be mediated, at least in part, by inhibition of the zinc-containing heme biosynthetic enzyme delta-aminolevulinate dehydratase (ALA-D). In the present study we investigated the effects of lead and/or zinc treatment during the second stage of rapid postnatal brain development on brain, kidney and blood ALA-D specific activity, as well as the negative geotaxis behavior of rats. Eight-day-old Wistar rats were injected intraperitoneally with saline, lead acetate (8 mg/kg) and/or zinc chloride (2 mg/kg) daily for five consecutive days. Twenty-four hours after treatment, ALA-D activity was determined in the absence and presence of DL- |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Lead acetate; Zinc chloride; Delta-aminolevulinate dehydratase; Negative geotaxis; Postnatal brain growth. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600014 |
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| Oliveira,B.M.; Romanha,A.J.; Alves,T.M.A.; Viana,M.B.; Zani,C.L.. |
| A procedure is described for the rapid determination of the intra-erythrocyte concentration of 6-mercaptopurine (6-MP) and its metabolites, 6-thioguanine nucleotides (6-TGN) and 6-methylmercaptopurine (6-MMP). Erythrocytes (8 x 10(8) cells) in 350 µl Hanks solution containing 7.5 mg dithiothreitol were treated with 50 µl 70% perchloric acid. The precipitate was removed by centrifugation (13,000 g) and the supernatant hydrolyzed at 100°C for 45 min. After cooling, 100 µl was analyzed directly by HPLC using a Radialpack Resolve C18 column eluted with methanol-water (7.5:92.5, v/v) containing 100 mM triethylamine. 6-TG, 6-MP and the hydrolysis product of 6-MMP, 4-amino-5-(methylthio)carbonyl imidazole, were monitored at 342,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acute lymphoblastic; Ukemia; Chemotherapy; 6-Mercaptopurine; Compliance; Children. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500004 |
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| Registros recuperados: 14 | |
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