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| Registros recuperados: 24 | |
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| Ventura-Juncá,Patricio; Irarrázaval,Isabel; Rolle,Augusto J; Gutiérrez,Juan I; Moreno,Ricardo D; Santos,Manuel J. |
| The advent of in vitro fertilization (IVF) in animals and humans implies an extraordinary change in the environment where the beginning of a new organism takes place. In mammals fertilization occurs in the maternal oviduct, where there are unique conditions for guaranteeing the encounter of the gametes and the first stages of development of the embryo and thus its future. During this period a major epigenetic reprogramming takes place that is crucial for the normal fate of the embryo. This epigenetic reprogramming is very vulnerable to changes in environmental conditions such as the ones implied in IVF, including in vitro culture, nutrition, light, temperature, oxygen tension,... |
| Tipo: Journal article |
Palavras-chave: In vitro fertilization (IVF) in mammals; Epigenetics; Developmental alterations; Bioethical implications. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100068 |
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| Tetzner,Tatiane Almeida Drummond; Saraiva,Naiara Zoccal; Perecin,Felipe; Niciura,Simone Cristina Méo; Ferreira,Christina Ramires; Oliveira,Clara Slade; Garcia,Joaquim Mansano. |
| Embryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine embryo; In vitro culture; Ovalbumin; Protein source. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011001000010 |
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| KAWASE, Yosuke; TACHIBE, Takanori; HANI, Toshio; TATEISHI, Hiromi; JISHAGE, Kou-ichi; SUZUKI, Hiroshi; 鈴木, 宏志. |
| Zona incision using a piezo-micromanipulator (ZIP) has been demonstrated to be effective for in vitro fertilization (IVF) using cryopreserved C57BL/6 spermatozoa. In this study, ZIP oocytes inseminated with frozen-thawed genetically modified C57BL/6J or FVB mice spermatozoa (21 lines) showed fertilization rates of 22-75% and live fetus rates of 8-49%. In 6 of the lines, the fertilization rates for oocytes compared with ZIP (42-75%) were significantly higher than that of nontreated oocytes (0-50%). Using only 90 oocytes for IVF with ZIP, 5 breeding pairs were produced from cryopreserved genetically modified mice spermatozoa. Our results indicate that application of the ZIP technique is... |
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Palavras-chave: Frozen-thawed spermatozoa; IVF; ZIP. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3017 |
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| Gaudron, S. M.; Hourdez, S.; Olu, Karine. |
| We investigated two gonochoristic species of annelid polychaetes (one siboglinid and one polynoid) from cold seeps that ranged from 525 m to 3300 m in depth (Guiness, Worm Hole and Regab pockmarks) on the Gabon and Congo continental margins (Gulf of Guinea). Different aspects of gametogenesis (oocyte diameter, presence of ovisac, spermatozoa shape, and fecundity), fertilization (in vitro fertilization experiments: IVF) and embryogenesis (cleavage rate) were studied. The sampled siboglinid was a new species of Lamellibrachia and the second population of this genus in the Eastern Atlantic. Mean oocyte diameter was about 100 µm and fully-grown primary oocytes were stored in an ovisac, as in other studied siboglinids. The... |
| Tipo: Text |
Palavras-chave: Branchipolynoe; Cold seeps; Dispersal; Lamellibrachia; Polynoid; Reproduction; Siboglinid. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00405/51644/52192.pdf |
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| SANTOS, S. A.; COSTA, C.; POTT, A.; CRISPIM, S. M. A.; SORIANO, B. M. A.; ALVAREZ, J. M.; ORTIZ, A. G.. |
| Os bovinos são criados em sistema de pastejo contínuo no Pantanal, onde tem a oportunidade de selecionar as espécies forrageiras, cuja qualidade das pastagens depende da proporção das espécies forrageiras preferidas. Este trabalho tem como objetivo avaliar o grau de preferência das forrageiras, bem como avaliar o índice de valor forrageiro das áreas de campo limpo e baixadas da fazenda Nhumirim, subregião da Nhecolândia, Pantanal. Em uma unidade de pastejo da fazenda Nhumirim foram efetuados o levantamento das espécies existentes e observação direta do comportamento de consumo por bovino, com o intuito de classificar as espécies da seguinte forma: preferida (P), desejável (D) e indesejável (I). Considerando a quantidade relativa de espécies P, D e I nas... |
| Tipo: Folhetos |
Palavras-chave: Bovine; Pasture; Quality; Bovino; Dieta; Pastagem; Qualidade; Pantanal; Diet; Cattle. |
| Ano: 2003 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/811099 |
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| Jagt, J.W.M.; Jagt-Yazykova, E.A.. |
| A synthesis of the stratigraphy of the Maastrichtian Stage in its extended type area, that is, southern Limburg (the Netherlands), and adjacent Belgian and German territories, is presented with a brief historical overview. Quarrying activities at the large quarry complex of ENCI-HeidelbergCement Group will officially come to an end on July 1, 2018. However, the stratotype section below the Lichtenberg farmstead and directly behind the main office building at the Lage Kanaaldijk (Maastricht), will be preserved, as will various faces within the quarry complex. Strata of Maastrichtian age include the Vijlen, Lixhe 1-3 and Lanaye members of the Gulpen Formation, as well as the Valkenburg, Gronsveld, Schiepersberg, Emael, Nekum and Meerssen members of the... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Late Cretaceous; Maastrichtian; Type area; Localities; Stratigraphy; 38.16; 38.22. |
| Ano: 2012 |
URL: http://www.repository.naturalis.nl/record/428924 |
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| Santana,B.B.; Sobral,G.G.; Gomes,E.T.; Batista,A.M.; Teixeira,L.P.R.; Tavares,K.C.S.; Bertolini,M.; Carneiro,G.F.. |
| ABSTRACT The aim of this work was to evaluate the effect of the Rolipram during the maturation of bovine oocytes and gene expression of embryos produced in vitro. Bovine ovaries were collected in slaughterhouse. The COCs were selected and divided into 5 groups: Control 0 time; Control: IVM for 24 hours; Rolipram treatments with IVM blocking for 24 hours in maturation medium containing (100, 150 and 200µM). After 24 hours all groups were reseated in IVM for another 24 hours. Subsequently COCs were subjected to the same IVM system and fertilized, being checked for cleavage post fertilization and for blastocyst. In addition, performed expression of the following genes: Mater, BMP15 and Bax. No difference was found in gene expression. Of oocytes evaluated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Rolipram; IVF; IVM; Oocyte. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000501433 |
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| Oliveira,F.D.; Sangalli,J.R.; Meirelles,F.V.; Perecin,F.; Silva Filho,P.P.C.; Watanabe,Y.F.; Miglino,M.A.; Assis Neto,A.C.. |
| In cattle, embryo development is characterized by the appearance of two distinct cell layers, the trophectoderm and the inner cell mass. The latter will undergo differentiation to form the embryonic disc consisting of the epiblast and hypoblast. The aim of this study was to ultrastructurally characterize the bovine embryo from different in vitro production techniques, with emphasis on trophectoderm and inner cell mass cells. Bovine embryos on day 7 (conception = D1) of pregnancy, derived via in vitro production techniques, were fixed for light and transmission electron microscopy processing. Results suggested that embryos produced by nuclear transfer of somatic cells and parthenogenesis showed significant changes in macroscopic and microscopic structure.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo; Blastocyst; In vitro production; Ultrastructure. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000601483 |
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| Sepúlveda,Soledad J; Portella,Jimmy R; Noriega,Luis P; Escudero,Ernesto L; Noriega,Luis H. |
| The aim of this study was to review the experience and outcomes of assisted reproduction cycles with embryos grown up to day 5 of development, comparing different parameters according to the ages of the patients. We retrospectively studied 1,874 assisted reproduction cycles where embryo culture was extended up to the fifth or sixth day of development. All IVF and ICSI cycles were included, comparing, according to patient age, the following rates: blastocyst formation, pregnancy, implantation and abortion. As control, we analyzed cycles with donated oocytes from young donors (OD). The number of embryos reaching the blastocyst stage is similar in all groups of patients. Only the OD group was different in terms of blastocyst... |
| Tipo: Journal article |
Palavras-chave: Blastocyst; Long-term culture; Pregnancy rate; Implantation rate. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200012 |
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| Talbot,Prue; Lin,Sabrina. |
| Numerous studies have repeatedly shown that women who smoke experience problems establishing and maintaining pregnancies, and recent work has further demonstrated that the in utero effects of smoke may not be manifested until months or even years after birth. The purpose of this review is to examine the recent literature dealing with the effects of cigarette smoke on the earliest stages of human prenatal development. Studies in this area have included the use of animal models, patients undergoing in vitro fertilization, and embryonic stem cell models. Events leading to fertilization, such as cumulus expansion, hyperactivation of sperm motility, and oocyte pick-up by the oviduct are all impaired by smoke exposure in animal models. Steps crucial to... |
| Tipo: Journal article |
Palavras-chave: Cigarette smoke; Embryos; Fertilization; IVF; Stem cells; Toxicology. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200011 |
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| Hidalgo,L.G; Catena,M; Petreigne,E.H; Seguí,R. |
| El objetivo fue analizar algunas variables pasibles de afectar la tasa de preñez en 20 establecimientos de cría de la Cuenca del Salado (subzona IV-F) durante el servicio 2009-2010. Se realizó la palpación transrectal (abril a junio de 2010) de 107 rodeos (19529 hembras) en vacas (primíparas y multíparas) y vaquillonas de 15 y 27 meses de edad. Se registró la cantidad de preñadas y vacías, condición corporal, sanidad del rodeo y edad de los vientres y se evaluó sanitariamente a los toros. Se concluye que el promedio de preñez para todas las categorías fue de 85%. En el 23,7% de los vientres sin presencia de enfermedades, la condición corporal fue igual o menor a 2,5 y la preñez de 80,7%. En rodeos con enfermedades... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rodeos de cría; Cuenca del Salado; Porcentaje de preñez. |
| Ano: 2011 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982011000100009 |
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| DP,Alves; MB,Carneiro; IVF,Martins; CC,Bernardo; DM,Donatele; Pereira Júnior OS,; BR,Almeida; BR,Avelar; AGC,Leão. |
| Fasciolosis is a disease that affects the liver parenchyma and bile ducts of numerous animals, including humans, which causes economic losses and threatens public health. The present work aimed at reporting the distribution and factors connected with Fasciola hepatica infection in cattle in ten municipalities in southern Espírito Santo, Brazil, between 2008 and 2009. During this period, 50 rural properties in ten cities were analyzed and fecal samples from 10% of the cattle on each farm were collected. Of the 717 samples analyzed, 154 (21.33%) presented Fasciola hepatica eggs. As for the 50 rural properties studied, 32 (64%) had animals positive for fasciolosis. Throughout the evaluation, it was observed that 42 (84%) rural properties had flooded areas, 33... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fasciolosis; Epidemiology; Ruminants. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300006 |
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| Ruiz López,Salvador; Souza,José Camisão de; Zaraza González,Javier; De Ondiz Sánchez,Aitor; Romero-Aguirregomezcorta,Jon; Carvalho,Rafaela Rodrigues de; Rath,Detlef. |
| The objective was to investigate the effects of sex-sorting on early fertilization events in ovum pick up (OPU) derived oocytes fertilized in vitro with frozen-thawed sperm at different co-incubation lengths. Eighty-four OPU sessions were carried out in 18 cyclic, dry and non-stimulated Holstein Friesian and German black pied cows. Ovum pick up oocytes were matured in vitro for 24 hours and fertilized with frozen-thawed sex sorted or unsorted sperm from the same ejaculate. Fertilization was achieved by two experimental protocols: 1) short gamete co-incubation length: 4, 8 and 12 hours; and 2) long gamete co-incubation length: 18 and 24 hours. After in vitro fertilization, ova were fixed and stained to identify early fertilization events. Sperm penetration,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine IVF; Co-incubation length; Early fertilization; Ovum pick up; Sex-sorted sperm. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013001000005 |
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| Silva, Ricardo Tomaz da; Faculdade de Medicina Veterinária, Universidade Professor Antônio Carlos, Campus Uberlândia; Mendes Júnior, José Olavo Borges; Instituto de Estudos Avançados José Caetano Borges, Universidade de Uberaba, Uberaba; Beletti, Marcelo Emílio; Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia. |
| The objective of this work was to evaluate the relationship between sperm head morphometry and chromatin packaging with the cleavage ratio and blastocyst formation during in vitro fertilization programs (IVF). Data of 38 IVFs using semen of zebu bulls with normal spermogram, carried out at two laboratories in Uberaba, Minas Gerais, Brazil, were used in this study. Sperm smears stained with toluidine blue and analyzed by computer image analysis were used to evaluate the morphometry and chromatin packaging. The packaging intensity and homogeneity of sperm chromatin were evaluated. The results made it possible to conclude that light variations in the head morphometry and chromatin packaging had little influence on the... |
| Tipo: Análise laboratorial |
Palavras-chave: 5.05.04.00-2 Reprodução Animal azul de toluidina; Bovinos; Cromatina; Espermatozóides; Fertilização in vitro. Reprodução Animal toluidine blue; Bovines; Chromatin; Spermatozoa; In vitro fertilization.. |
| Ano: 2009 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/1002 |
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| Registros recuperados: 24 | |
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