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Stable expression and characterization of a fungal pectinase and bacterial peroxidase genes in tobacco chloroplast Electron. J. Biotechnol.
Espinoza-Sánchez,Edward Alexander; Álvarez-Hernández,Marianela Hazel; Torres-Castillo,Jorge Ariel; Rascón-Cruz,Quintín; Gutiérrez-Díez,Adriana; Zavala-García,Francisco; Sinagawa-García,Sugey Ramona.
Background The high capacity of chloroplast genome response to integrate and express transgenes at high levels makes this technology a good option to produce proteins of interest. This report presents the stable expression of Pectin lyase (PelA gene) and the first stable expression of manganese peroxidase (MnP-2 gene) from the chloroplast genome. Results pES4 and pES5 vectors were derived from pPV111A plasmid and contain the PelA and MnP-2 synthetic genes, respectively. Both genes are flanked by a synthetic rrn16S promoter and the 3'UTR from rbcL gene. Efficient gene integration into both inverted repeats of the intergenic region between rrn16S and 3'rps'12 was confirmed by Southern blot. Stable processing and expression of the RNA were confirmed by...
Tipo: Journal article Palavras-chave: Cell wall; Chloroplast genetic transformation; Hydrolytic enzyme; Nicotiana tabacum.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300004
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