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Registros recuperados: 17
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Involvement of a Novel Genistein-Inducible Multidrug Efflux Pump of Bradyrhizobium japonicum Early in the Interaction with Glycine max (L.) Merr OAK
Takeshima, Keisuke; Hidaka, Tatsuo; Min Wei2); Yokoyama, Tadashi; Minamisawa, Kiwamu; Mitsui, Hisayuki; Itakura, Manabu; Kaneko, Takakazu; Tabata, Satoshi; Saeki, Kazuhiko; Oomori, Hirofumi; Tajima, Shigeyuki; Uchiumi, Toshiki; Abe, Mikiko; Tokuji, Yoshihiko; Ohwada, Takuji; 得字, 圭彦; 大和田, 琢二.
The early molecular dialogue between soybean and the bacterium Bradyrhizobium japonicum is crucial for triggering their symbiotic interaction. Here we found a single large genomic locus that is widely separated from the symbiosis island and was conspicuously induced within minutes after the addition of genistein. This locus (named BjG30) contains genes for the multidrug efflux pump, TetR family transcriptional regulator, and polyhydroxybutyrate (PHB) metabolism. The induction of BjG30 by genistein was competitively inhibited by daidzein, although both genistein and daidzein are soybean-derived inducers of nodulation (nod) genes. Such a differential expression pattern is also observed in some legume-derived flavonoids, which structurally differ in the...
Palavras-chave: Bradyrhizobium; Genistein; Multidrug efflux pump; TetR family transcriptional regulator.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3945
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根粒菌感染に介在する特異的シグナル OAK
大和田, 琢二; OWADA, Takuji.
※「化学と生物」p258-268「今日の話題」内の論文
Ano: 1997 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4154
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Osmoregulation of Bacteria OAK
大和田, 琢二; 匂坂, 勝之助; OWADA, Takuji.
J-stageにて公開
Ano: 1990 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4153
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ウレタンを吸着・分解する微生物とそのスクリーニング OAK
大和田, 琢二.
Palavras-chave: ウレタン; 微生物; 分解.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2883
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Rhizobium fredii USDA191の過酸化水素耐性変異株の根粒形成及び窒素固定機能について OAK
大和田, 琢二; 三浦, 賢紀; 南, 里栄; 白川, 雪香; 井川, 香子; 佐藤, 哲也; OHWADA, Takuji; MIURA, Takanori; MINAMI, Rie; SHIRAKAWA, Yukika; IGAWA, Keiko; SATO, Tetsuya.
Palavras-chave: 根粒菌; 過酸化水素(H2O2); トランスポゾン(Tn5); ペルオキシダーゼ; 根粒形成; Rhizobium; Hydrogen peroxide (H2O2); Transposon (Tn5); Peroxidase; Nodulation.
Ano: 1998 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1287
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根粒菌の増殖と代謝系に及ぼすヒドロキシリシンの影響 OAK
大和田, 琢二; 井川, 香子; 佐藤, 哲也; OHWADA, Takuji; IGAWA, Keiko; SATO, Tetsuya.
Palavras-chave: Rhizobium; バクテロイド; ヒドロキシリシン(Hyl); グルタミン酸; 窒素固定; Rhizobium; Bacteroid; Hydroxylysine; Glutamic acid; Nitrogen fixation.
Ano: 1998 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1294
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低温で増殖する土壌微生物の単離と利用 OAK
大和田, 琢二.
平成22年度 Ⅰ.基盤研究部門
Ano: 2012 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3374
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Improved Leavening Ability of a Wild Yeast, Saccharomyces cerevisiae AK46 2-deoxyglucose Resistant Mutant OAK
Mikumo, Dai; Takaya, Masahiro; Orikasa, Yoshitake; Ohwada, Takuji; 折笠, 善丈; 大和田, 琢二.
The wild yeast Saccharomyces cerevisiae AK46 was isolated from cherry fruits and commercialized as baker's yeast. We isolated a 2-deoxyglucose (2-DOG)-resistant mutant of AK46 (MCD4) and evaluated its leavening ability in bread dough. Our findings show that the maltose utilization of MCD4 was significantly enhanced in the presence of glucose and α-glucosidase activity was increased by approx. 1.6-fold compared to that of AK46, indicating release from catabolite repression. In addition, baking performance results using the sponge dough and no-time dough methods showed that the CO2 production rate of MCD4 was increased by approx. 1.6-fold (sponge and dough method) and the specific volume of bread was increased by 6.3% and 7.8%, respectively. These results...
Palavras-chave: Bread dough; Saccharomyces cerevisiae; 2-deoxyglucose; AK46; Leavening.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4368
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ポリウレタン吸着・分解菌の単離とその性質 OAK
大和田, 琢二.
Palavras-chave: ポリウレタン分解; 吸着; 微生物; 廃棄物.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2899
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Bradyrhizobium japonicumカタラーゼ遺伝子のクローニング方法の確立 OAK
白川, 雪香; 井川, 香子; 佐藤, 哲也; 大和田, 琢二; SHIRAKAWA, Yukika; IGAWA, Keiko; SATO, Tetsuya; OHWADA, Takuji.
Palavras-chave: Bradyrhizobium; カタラーゼ; クローニング; In vitro パッケージング; Bradyrhizobium; Catalase; Cloning; In vitro packaging.
Ano: 1998 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1288
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低温で増殖する土壌微生物の単離と利用 OAK
大和田, 琢二.
平成21年度 Ⅰ.基盤研究部門
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3345
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第9回国際窒素固定会議出席,発表及び米国コロラド大学(ボールダー校)における研究打合わせ OAK
大和田, 琢二.
Ano: 1993 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/525
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食料生産における根粒菌の有効利用について OAK
大和田, 琢二.
Ano: 1992 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/510
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Rhizobium fredii USDA191の過酸化水素感受性とカタラーゼ, ペルオキシダーゼ活性について OAK
大和田, 琢二; 三浦, 賢紀; 南, 里栄; 白川, 雪香; 井川, 香子; 佐藤, 哲也; OHWADA, Takuji; MIURA, Takanori; MINAMI, Rie; SHIRAKAWA, Yukika; IGAWA, Keiko; SATO, Tetsuya.
Palavras-chave: 根粒菌; 過酸化水素(H2O2); カタラーゼ; ペルオキシダーゼ; Rhizobium; Hydrogen peroxide(H2O2); Catalase; Peroxidase.
Ano: 1998 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1286
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Improved ethanol production by cheese whey-based simultaneous saccharifying fermentation using  Kluyveromyces marxianus KD-15 released from catabolite repression OAK
折笠, 善丈; 佐藤, 遥子; 大和田, 琢二; Orikasa, Yoshitake; Sato, Haruko; Ohwada, Takuji.
 チーズホエイを原料に用いた焼酎製造法を検討するため,カタボライト抑制解除株 Kluyveromyces marxianus KD-15 を用いて,チーズホエイを加えた糖化同時発酵におけるエタノール生産量を改良した。Aspergillus luchuensis あるいは A. oryzae で作成された麹と酵母 3 株(K. marxianus NBRC1963(親株)と K. marxianus KD-15, Saccharomyces cerevisiae NBRC10515)を組み合わせ,培養混合物中の pH,グルコースとラクトース含量,α アミラーゼと糖化力,並びにエタノール含量を経時的に21日間測定した。その結果,K. marxianus KD-15はいずれの麹との組み合わせでもエタノール生産量が他の酵母株よりも有意に高く,A. luchuensis との組み合わせでは,親株,及び S. cerevisiae NBRC10515 より約30%向上した。α-アミラーゼ,および糖化力は培養とともに低下傾向を示したが,K. marxianus KD-15 はいずれの麹との組み合わせでもラクトース消費量が最も高かったことから,K. marxianus KD-15 による高いエタノール生産は,デンプン由来のグルコースよりもチーズホエイ由来のラクトース消費量の影響を受けると考えられた。以上の結果から,K. marxianus KD-15 はデンプン質の混合原料の存在下でも高いチーズホエイの資化とエタノール生成能を有し,チーズホエイを原料とした焼酎製造への有用性が示された。
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4371
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低温で増殖する土壌微生物の単離と利用 OAK
大和田, 琢二.
平成23年度 Ⅰ.基盤研究部門
Ano: 2012 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3391
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「プラスチック関連ポリマーの分解に関わる微生物のスクリーニング」の課題で得られた研究成果を取りまとめるための打合せ OAK
大和田, 琢二.
平成20年10月4日-平成20年10月11日
Palavras-chave: 微生物; プラスチック分解; ポリウレタン.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2550
Registros recuperados: 17
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