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| 松嶋, 卯月; 武田, 一夫; Tanino, Karen K.. |
| In order to clarify the role of the vascular system for creating ice segregation, the water pathway through the stem of Keisukea japonica and the freezing front of ice segregation were investigated. Harvested stems of Keisukea japonica were used as plant samples. The water pathway was visualized with a 2.5mM fluorescein solution. After ice segregation was induced, the stem was tightly fixed on a microtome that cross sectioned the stem every 40μm. The light source with excitation filter illuminated the stem cross section. Fluorescence emission was captured by using CCD camera with fluorescent filter. Three-dimensional fluorescent image was reconstructed from the series of captured images. By observing horizontal cross sections, fluorescent illumination... |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4508 |
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