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| Registros recuperados: 37 | |
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| 福田, 健二; 浦島, 匡; Fukuda, Kenji; Urashima, Tadasu. |
| Lactic acid bacteria (LAB) are commonly used in food industries，especially for the production of fermented dairy products. During fermentation process，some dairy LAB produce exopolysaccharide(EPS)，providing good mouthfeel properties to the products by modifying their textures. In general， EPS is divided into two groups based on their monosaccharide composition; homo-EPS and hetero-EPS. It is believed that the major physiological function of EPS is the first line of biological defense against phagocytosis，phage attack， antibiotics，toxic metal ions，and physical stresses such as desiccation and osmotic stress. Besides，EPS from dairy LAB exhibits bioactive characteristics，e.g.，anti-microbial，anti-tumoral，hypotensive，prebiotic，immuno-stimulative，and... |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3080 |
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| Fukuda, K; Senda, A; Ishii, T; Urashima, T; Morita, M; Terabayashi, T; 福田, 健二; 浦島, 匡. |
| Using a combination of PAGE and mass spectrom etry for protein identification，we obtained evidence that a putative odorant-binding protein，designated hypothetical protein LOC517854，occurs in bovine colostrum. This protein，termed as a putative bovine colostral odorant-binding protein (bcOBP)， consists of 172 AA residues，including a putative 16-AA signal peptide. The theoreticalis oelectric point value and molecular mass of the full-length sequence of bcOBP were calculated to be 4.57 and 19604.18，respectively. The highest sequence similarity (83%) was observed with a potential pheromone transporter，Allergen Bos d 2. An odorant-binding protein derived from bovine nasal mucosa showed relatively low sequence similarity(52%) against bcOBP. Its biological... |
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Palavras-chave: Colostrum; Lipocalin; Odorant-binding protein; Pheromone transport. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2674 |
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| Goto, Kohta; Fukuda, Kenji; Senda, Akitsugu; Saito, Tadao; Kimura, Kazumasa; Glander, Kenneth E.; Hinde, Katie; Dittus, Wolfgang; Milligan, Lauren A.; Power, Michael L.; Oftedal, Olav T.; Urashima, Tadasu; 浦島, 匡; 福田, 健二. |
| Human and great ape milks contain a diverse array of milk oligosaccharides, but little is known about the milk oligosaccharides of other primates, and how they differ among taxa. Neutral and acidic oligosaccharides were isolated from the milk of three species of Old World or catarrhine monkeys (Cercopithecidae: rhesus macaque (Macaca mulatta), toque macaque (Macaca sinica) and Hamadryas baboon (Papio hamadryas)) and three of New World or platyrrhine monkeys (Cebidae: tufted capuchin (Cebus apella)and Bolivian squirrel monkey (Saimiri boliviensis); Atelidae: mantled howler (Alouatta palliata)). The milks of these species contained 6-8% total sugar, most of which was lactose: the estimated ratio of oligosaccharides to lactose in Old World monkeys (1:4 to... |
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Palavras-chave: Old world monkey; New world monkey; Milk oligosaccharide; Rhesus macaque; Toque macaque; Baboon; Capuchin; Mantled howler; Squirrel monkey; N-glycolylneuraminic acid. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3144 |
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| NOZAKI, Hirofumi; WIJAYAGUNAWARDANE, Missaka P.B.; KODITUWAKKU, Suranga P.; YOSHIDA, Takashi; NAKAMURA, Tadashi; ARAI, Ikichi; URASHIMA, Tadasu; MIYAMOTO, Akio; 中村, 正; 浦島, 匡; 宮本, 明夫. |
| N-acetylglucosaminyltransferase I (GnT I; EC 2.4.1.101), which catalyzes the first step in the conversion of oligomannose to complex or hybrid N-glycans of glycoproteins, was found in media cultured with bovine oviduct epithelial cells (BOEC) obtained from non-pregnant cows during the follicular phase. Combined treatment with specific hormones increased GnT I release from BOEC. Luteinizing hormone (LH; 10 ng/ml) alone slightly, but together with 17β-estradiol (E2; 1 ng/ml), synergistically increased GnT I activity. Vascular endothelial growth factor (VEGF) and tumor necrosis factor (TNF) α, which have been shown to have their highest activities in the bovine oviduct during the periovulatory period, also increased in GnT I activity. This study provides the... |
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Palavras-chave: Bovine; Luteinizing hormone (LH); N-acetylglucosaminyltransferase I; Oviduct; Tumor necrosis factor (TNF) α; Vascular endothelial growth factor (VEGF). |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2978 |
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| 仙田, 晶嗣; 中村, 正; 浦島, 匡; 荒井, 威吉. |
| 生乳中の酵母の動態を明らかにすることを目的として，帯広畜産大学と近郊の酪農家3戸で飼養している健康なホルスタイン種乳牛（体細胞数1×105cfu/ml以下）の乳頭表皮，分房乳，バルク乳およびローリー乳中の酵母数の月別変動について調べた．各試料は1999年7月から2000年6月までの1年間，それぞれ毎月1回ずつ採取した．生乳中の酵母の検出率と平均酵母数はそれぞれ順に，乳頭表皮では70.1％，52cfu/cm2，分房乳は47.9％，1.8cfu/ml，バルク乳は100％，28cfu/mlおよびローリー乳では100％，66cfu/mlであった．乳頭表皮および各種類の生乳中において90％以上を占める酵母数の水準は，乳頭表皮が150cfu/cm2以下（90.8％），分房乳が10cfu/ml以下（94.4％），バルク乳が100cfu/ml以下（97.8％）およびローリー乳が150cfu/ml以下（91.7％）であると考えられた．生乳中の酵母数は夏から秋期に高く，冬から春期に低くなる季節的な変動傾向が認められた．健康な乳牛の分房乳中では，主な乳房炎起因菌（C. parapsilosis, C. lambica, C. catenulata, C. rugosa）は夏期に検出されたが，冬から春期には検出されなかった． The objective of this study was to investigate the seasonal incidences of yeast and the seasonal changes of yeast counts (cfu/ml) in a high quality raw milk produced by healthy Holstein cows (≤1×105 somatic... |
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Palavras-chave: Yeast in milk; The incidence of yeast; The seasonal changes of yeast counts. |
| Ano: 2003 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2640 |
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| 中村, 正; 菅井, 理子; 男澤, 綾; 有賀, 秀子; 小疇, 浩; キュラ, キーユキア; 荒井, 威吉; 浦島, 匡; Nakamura, Tadashi; Sugai, Michiko; Nakamura, Aya; Ariga, Hideko; Koaze, Hiroshi; Kiiyukia, Cura; Arai, Ikichi; Urashima, Tadasu. |
| Ano: 1999 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1877 |
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| 有賀, 秀子; 高橋, セツ子; 倉持, 泰子; 浦島, 匡; 筒井, 静子; Ariga, Hideko; Takahashi, Setsuko; Kuramochi, Yasuko; Urashima, Tadasu; Tsutsui, Shizuko. |
| Ano: 1988 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1848 |
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| Nozaki, Hirofumi; Miyamoto, Akio; Hayashi, Ken-Go; Matsui, Motozumi; Yoshida, Takashi; Nakamura, Tadashi; Arai, Ikichi; Urashima, Tadasu; 宮本, 明夫; 松井, 基純; 中村, 正; 浦島, 匡. |
| The activity of α3-D-mannoside-β-1,2-N-acetylglucosaminyltransferase I (GnT I; EC 2.4.1.101), which catalyzes the first step in the conversion of oligomannose to complex or hybrid N-glycans of glycoproteins, was detected in bovine follicular fluid (bFF). The GnT I activity in bFF had a pH optimum of 5.8 and an absolute requirement for either Co2+, Mn2+, or Mg2+, the activity being stimulated by these cations in the above order. The apparent Km value for α1-3α1-6 mannopentaose of GnT I in bFF was 2.17 mM. The substrate specificity for the GnT I activity decreased in the following order: α1-3α1-6 mannopentaose> α1-3α1-6 mannotriose>α1-3 mannobiose. The GnT I activity in bFF from large atretic follicles was significantly higher than in that from large... |
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Palavras-chave: N-acetylglucosaminyltransferase I; Dominant follicle; Atretic follicle; Follicular fluid; Bovine. |
| Ano: 2004 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4185 |
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| SUJAYA, I-Nengah; MIKUMO, Dai; ORIKASA, Yoshitake; URASHIMA, Tadasu; ODA, Yuji; 浦島, 匡; 小田, 有二; 折笠, 善丈. |
| Twenty-two strains of Saccharomyces cerevisiae isolated from tape-mash for the production of brem, a traditional rice wine from Bali, Indonesia, were divided into three groups (I, II, and III) based on the sequences of the rDNA spacer region and SUC2 gene. DNA analyses suggested that the seventeen strains in groups II and III were taxonomically closer to baking strains than the five strains in group I. These differences were reflected in their leavening ability in dough with and without addition of 5% sucrose, and their α-glucosidase activity, although most of the strains did not leaven dough with addition of 30% sucrose. Strain S-10 in group II showed a high rate of CO2 production from sponge dough without addition of sugar and consumed fermentable sugar... |
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Palavras-chave: Brem; SUC2 gene; Leavening; Breadmaking; Dough. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3573 |
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| Fukuda, K.; Yamamoto, A.; Ganzorig, K.; Senda, A.; Saito, T.; Urashima, T.; 福田, 健二; 浦島, 匡; Khuukhenbaatar, J.. |
| Bactrian camel milk and colostrum are commonly used as foods in Mongolia, whose people believe that these products promote human health. It has been hypothesized that milk oligosaccharides are biologically significant components of human milk, acting as receptor analogs that inhibit the attachment of pathogenic microorganisms to the colonic mucosa, and as prebiotics, which stimulate the growth of bifidobacteria within the infant colon. To evaluate their biological significance, we studied the oligosaccharides present in samples of Bactrian camel milk and colostrum. Using 1H-nuclear magnetic resonance spectroscopy, we identified and characterized the following oligosaccharides of camel colostrum: Gal(β1–4)[Fuc(α1–3)]Glc (3-fucosyllactose),... |
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Palavras-chave: Bactrian camel; Milk oligosaccharide; Colostrum; Sialyl oligosaccharide. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3093 |
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| Registros recuperados: 37 | |
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