|
|
|
| Registros recuperados: 37 | |
|
|
| 仙田, 晶嗣; 中村, 正; 浦島, 匡; 荒井, 威吉. |
| 生乳中の酵母の動態を明らかにすることを目的として，帯広畜産大学と近郊の酪農家3戸で飼養している健康なホルスタイン種乳牛（体細胞数1×105cfu/ml以下）の乳頭表皮，分房乳，バルク乳およびローリー乳中の酵母数の月別変動について調べた．各試料は1999年7月から2000年6月までの1年間，それぞれ毎月1回ずつ採取した．生乳中の酵母の検出率と平均酵母数はそれぞれ順に，乳頭表皮では70.1％，52cfu/cm2，分房乳は47.9％，1.8cfu/ml，バルク乳は100％，28cfu/mlおよびローリー乳では100％，66cfu/mlであった．乳頭表皮および各種類の生乳中において90％以上を占める酵母数の水準は，乳頭表皮が150cfu/cm2以下（90.8％），分房乳が10cfu/ml以下（94.4％），バルク乳が100cfu/ml以下（97.8％）およびローリー乳が150cfu/ml以下（91.7％）であると考えられた．生乳中の酵母数は夏から秋期に高く，冬から春期に低くなる季節的な変動傾向が認められた．健康な乳牛の分房乳中では，主な乳房炎起因菌（C. parapsilosis, C. lambica, C. catenulata, C. rugosa）は夏期に検出されたが，冬から春期には検出されなかった． The objective of this study was to investigate the seasonal incidences of yeast and the seasonal changes of yeast counts (cfu/ml) in a high quality raw milk produced by healthy Holstein cows (≤1×105 somatic... |
|
Palavras-chave: Yeast in milk; The incidence of yeast; The seasonal changes of yeast counts. |
| Ano: 2003 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2640 |
| |
|
|
| 荒井, 威吉; 中島, 景典; 丸山, 千弘玲; 中村, 正; 戸羽, 隆宏; 浦島, 匡; Arai, Ikichi; Nakajima, Keisuke; Maruyama, Chigure; Nakamura, Tadashi; Toba, Takahiro; Urashima, Tadasu. |
| Microflora of lactic acid bacteria and yeasts of Laban which had been made with bovine or ovine milk by traditional natural fermentation in Yemen，were studied. The mean of viable cell counts of microorganisms in Laban was 6.4 x10⁸ cfu/ml. The 60 strains of lactic acid bacteria and 24 strains of yeasts were isolated and identified. The percentages of strains on lactic acid bacteria flora were as follows: Lactococcus lactis subsp.lactis① was dominant in 88%. Lc. lactis subsp. lactis②，Leuconostoc mesenteroides subsp. mesenteroides，and Leuc. lactis were in 5，5，and 2%，respectively. It was recognized that the some characteristics of Lc. lactis subsp. lactis① of the dominant strain were different from those of Lc. lactis subsp.lactis②. The percentages of strains... |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2923 |
| |
|
|
| Fukuda, K; Senda, A; Ishii, T; Urashima, T; Morita, M; Terabayashi, T; 福田, 健二; 浦島, 匡. |
| Using a combination of PAGE and mass spectrom etry for protein identification，we obtained evidence that a putative odorant-binding protein，designated hypothetical protein LOC517854，occurs in bovine colostrum. This protein，termed as a putative bovine colostral odorant-binding protein (bcOBP)， consists of 172 AA residues，including a putative 16-AA signal peptide. The theoreticalis oelectric point value and molecular mass of the full-length sequence of bcOBP were calculated to be 4.57 and 19604.18，respectively. The highest sequence similarity (83%) was observed with a potential pheromone transporter，Allergen Bos d 2. An odorant-binding protein derived from bovine nasal mucosa showed relatively low sequence similarity(52%) against bcOBP. Its biological... |
|
Palavras-chave: Colostrum; Lipocalin; Odorant-binding protein; Pheromone transport. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2674 |
| |
|
| |
|
|
| 浦島, 匡; 上村, 祐介; 中村, 正; 佐々木, 基樹; Urashima, Tadasu; Uemura, Yuhsuke; Nakamura, Tadashi; Sasaki, Motoki. |
| ペンシルバニア紀(>3億1000万年前)に，有羊膜類であるSynapsidは他の系統から分化し，腺を発達させた。乳腺は毛包と会合した祖先型アポクリン腺から発達した。乳腺と毛の関係は単孔類の乳腺に保持されている。また，有袋類における乳腺毛の初期個体発生時の存在とその後の退化に，その証拠が残されている。単孔類の乳頭のない乳腺区分にあるmammo-ピロ-脂腺単位の密集した集団は，透水性を示す卵への水分や他の成分の補給のための構造的進化を反映しているかもしれない。ラクトースなどの成分は，初期Synapsidの祖先アポクリン腺から分泌されていたかもしれない。三畳紀のTherapsidは，複雑な栄養成分を含む乳を分泌し，卵サイズを小さくし，孵化時の新生子の未熟化を引き起こした。乳腺区分に生じた乳頭は，乳腺の毛を退化させた。 乳腺分泌は，羊皮状の卵殻をもつ卵を産むSynapsidで開始されたと考えられる。羊皮状の卵殻をもつ卵は，低湿度の空気にふれた際，急速に水分損失を被る。Synapsidは抱卵嚢の中で抱卵することで卵の乾燥を防いだが，これは母親の行動を制限した。羊皮状の卵殻をもつ卵は，殻を通して液体水分を補給することができる。乳腺分泌は，本来卵に水分を供給する手段として開始され，Cynodont (キノドン類)において内温性獲得の要因となった。Synapsidの卵は，ナトリウムやカルシウムイオンなどの栄養素を摂取した可能性がある。孔のある卵殻と2層性の卵黄膜は，子宮内での分泌物と抱卵時での乳腺分泌物の摂取を促進したと考えられる。 |
| Ano: 2004 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2924 |
| |
|
| |
|
|
| Fukuda, Kenji; Shi, Tala; Nagami, Kentaro; Leo, Fiame; Nakamura, Tadashi; Yasuda, Kumi; Senda, Akitsugu; Motoshima, Hidemasa; Urashima, Tadasu; 福田, 健二; 浦島, 匡. |
| A thermophilic lactic acid bacterium, Lactobacillus fermentum TDS030603, produced about 100 mg/L of EPS in purified form when grew in de Man-Rogosa-Sharpe (MRS) broth. The 1% (w/v) solution of the purified EPS was highly viscous, exhibiting an apparent 22 viscosity (ηapp) of 0.88 Pa s at a shear rate of 10/s. To investigate the impact of carbohydrate source on the production yield and chemical structure of EPS and the viscosity of EPS solution, a chemically defined medium (CDM) has been developed. Results of TLC, HPLC, 25 and 1H NMR spectroscopy indicated that the chemical structures of EPS released in MRS and in the CDM supplemented with glucose, galactose, lactose or sucrose were very similar. All the 1% solutions of EPSs released in CDMs were highly... |
|
Palavras-chave: Chemical structure; Chemically defined medium; Exopolysaccharide; Lactic acid bacteria; Viscosity. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2694 |
| |
|
|
| 荒井, 威吉; 仙田, 晶嗣; 山上, 貴礼; 無量谷, 亜希; 中村, 正; 浦島, 匡; ARAI, lkichi; SENDA, Akitsugu; YAMAGAMI, Kihiro; MURYOYA, Aki; NAKAMURA, Tadashi; URASHIMA, Tadasu. |
| The efficacy of the use of acidic electrolyzed water (AEW) for premilking teat cleaning and disinfection (TCD) was evaluated using a split-udder experimental design. The efficacy of AEW for TCD was compared with that of NaClO solution contained 200 ppm available chlorine (HClO). The viable cell counts levels of SPC，lactic acid bacteria，thermotrophilic bacteria and anaerobe (group A) which contaminated on teat skin were over 10³ cfu/cm²，whereas psychrotrophilic bacteria and coliforms (group B) were less 10² cfu/cm². After TCD with AEW and HClO，viable cell counts levels of the microorganisms of group A and group B were decreased to 2.8-7.6% and 10.0-14.5% respectively. On the other hand，the efficacy of AEW or HClO for TCD was almost constant throughout one... |
|
Palavras-chave: 強酸性電解水; 乳頭清拭; Acidic electrolyzed water; Premilking teat cleaning and disinfection. |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2993 |
| |
|
| |
|
| |
|
|
| Urashima, Tadasu; Nakamura, Tadashi; Ikeda, Akiko; Asakuma, Sadaki; Arai, Ikichi; Saito, Tadao; Oftedal, Olav T.; 浦島, 匡; 中村, 正. |
| Carbohydrates were extracted from a sample of milk from a mink, Mustela vison (Family Mustelidae). Free neutral and acidic oligosaccharides were isolated from the carbohydrate fraction and their chemical structures were compared with those of white-nosed coati (Nasua narica, Procyonidae) and harbour seal (Phoca vitulina, Phocidae) that we had studied previously. The ratio of free lactose to milk oligosaccharides was similar to that in milk of the white-nosed coati; in both species, this ratio was much lower than that in the milk of most eutherians. The neutral oligosaccharides of mink milk had alpha(1-3)-linked Gal or alpha(1-2)-linked Fuc residues at their non-reducing ends, as in the neutral oligosaccharides of white-nosed coati milk. Some of the neutral... |
|
Palavras-chave: Mink; Mustela vison; Mustelidae; Milk oligosaccharides; Neutral oligosaccharides; Sialyl oligosaccharides. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/729 |
| |
|
|
| Leo, Fiame; Hashida, Shukichi; Kumagai, Daiju; Uchida, Kenji; Motoshima, Hidemasa; Arai, Ikichi; Asakuma, Sadaki; Fukuda, Kenji; Urashima, Tadasu; 福田, 健二; 浦島, 匡. |
| A neutral exopolysaccharide produced in large amounts by Lactobacillus fermentum TDS030603 was purified from culture supernatants and its structure determined by monosaccharide analysis, methylation analysis and 1H-NMR spectroscopy. Optimum conditions for the production of this exopolysaccharide by the above bacterial strain, when cultured using cheese whey medium or MRS broth, were also investigated. The exopolysaccharide was found to be composed of D-galactose and D-glucose in the molar ratio of 1:2.5 and to consist of one major as well as one minor saccharide, both of which contained a tetrasaccharide repeating unit consisting of two α and two β anomers. The major saccharide contained non-reducing glucopyranose, O-3 substituted glucopyranose, O-6... |
|
Palavras-chave: Lactic acid bacteria; Exopolysaccharide; Lactobacillus fermentum TDS030603. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4183 |
| |
|
|
| Fukuda, K.; Yamamoto, A.; Ganzorig, K.; Senda, A.; Saito, T.; Urashima, T.; 福田, 健二; 浦島, 匡; Khuukhenbaatar, J.. |
| Bactrian camel milk and colostrum are commonly used as foods in Mongolia, whose people believe that these products promote human health. It has been hypothesized that milk oligosaccharides are biologically significant components of human milk, acting as receptor analogs that inhibit the attachment of pathogenic microorganisms to the colonic mucosa, and as prebiotics, which stimulate the growth of bifidobacteria within the infant colon. To evaluate their biological significance, we studied the oligosaccharides present in samples of Bactrian camel milk and colostrum. Using 1H-nuclear magnetic resonance spectroscopy, we identified and characterized the following oligosaccharides of camel colostrum: Gal(β1–4)[Fuc(α1–3)]Glc (3-fucosyllactose),... |
|
Palavras-chave: Bactrian camel; Milk oligosaccharide; Colostrum; Sialyl oligosaccharide. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3093 |
| |
|
|
| 福田, 健二; 浦島, 匡; Fukuda, Kenji; Urashima, Tadasu. |
| Lactic acid bacteria (LAB) are commonly used in food industries，especially for the production of fermented dairy products. During fermentation process，some dairy LAB produce exopolysaccharide(EPS)，providing good mouthfeel properties to the products by modifying their textures. In general， EPS is divided into two groups based on their monosaccharide composition; homo-EPS and hetero-EPS. It is believed that the major physiological function of EPS is the first line of biological defense against phagocytosis，phage attack， antibiotics，toxic metal ions，and physical stresses such as desiccation and osmotic stress. Besides，EPS from dairy LAB exhibits bioactive characteristics，e.g.，anti-microbial，anti-tumoral，hypotensive，prebiotic，immuno-stimulative，and... |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3080 |
| |
|
| |
|
| |
|
|
| Nozaki, Hirofumi; Miyamoto, Akio; Hayashi, Ken-Go; Matsui, Motozumi; Yoshida, Takashi; Nakamura, Tadashi; Arai, Ikichi; Urashima, Tadasu; 宮本, 明夫; 松井, 基純; 中村, 正; 浦島, 匡. |
| The activity of α3-D-mannoside-β-1,2-N-acetylglucosaminyltransferase I (GnT I; EC 2.4.1.101), which catalyzes the first step in the conversion of oligomannose to complex or hybrid N-glycans of glycoproteins, was detected in bovine follicular fluid (bFF). The GnT I activity in bFF had a pH optimum of 5.8 and an absolute requirement for either Co2+, Mn2+, or Mg2+, the activity being stimulated by these cations in the above order. The apparent Km value for α1-3α1-6 mannopentaose of GnT I in bFF was 2.17 mM. The substrate specificity for the GnT I activity decreased in the following order: α1-3α1-6 mannopentaose> α1-3α1-6 mannotriose>α1-3 mannobiose. The GnT I activity in bFF from large atretic follicles was significantly higher than in that from large... |
|
Palavras-chave: N-acetylglucosaminyltransferase I; Dominant follicle; Atretic follicle; Follicular fluid; Bovine. |
| Ano: 2004 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4185 |
| |
|
|
| Goto, Kohta; Fukuda, Kenji; Senda, Akitsugu; Saito, Tadao; Kimura, Kazumasa; Glander, Kenneth E.; Hinde, Katie; Dittus, Wolfgang; Milligan, Lauren A.; Power, Michael L.; Oftedal, Olav T.; Urashima, Tadasu; 浦島, 匡; 福田, 健二. |
| Human and great ape milks contain a diverse array of milk oligosaccharides, but little is known about the milk oligosaccharides of other primates, and how they differ among taxa. Neutral and acidic oligosaccharides were isolated from the milk of three species of Old World or catarrhine monkeys (Cercopithecidae: rhesus macaque (Macaca mulatta), toque macaque (Macaca sinica) and Hamadryas baboon (Papio hamadryas)) and three of New World or platyrrhine monkeys (Cebidae: tufted capuchin (Cebus apella)and Bolivian squirrel monkey (Saimiri boliviensis); Atelidae: mantled howler (Alouatta palliata)). The milks of these species contained 6-8% total sugar, most of which was lactose: the estimated ratio of oligosaccharides to lactose in Old World monkeys (1:4 to... |
|
Palavras-chave: Old world monkey; New world monkey; Milk oligosaccharide; Rhesus macaque; Toque macaque; Baboon; Capuchin; Mantled howler; Squirrel monkey; N-glycolylneuraminic acid. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3144 |
| |
|
|
| SUJAYA, I-Nengah; MIKUMO, Dai; ORIKASA, Yoshitake; URASHIMA, Tadasu; ODA, Yuji; 浦島, 匡; 小田, 有二; 折笠, 善丈. |
| Twenty-two strains of Saccharomyces cerevisiae isolated from tape-mash for the production of brem, a traditional rice wine from Bali, Indonesia, were divided into three groups (I, II, and III) based on the sequences of the rDNA spacer region and SUC2 gene. DNA analyses suggested that the seventeen strains in groups II and III were taxonomically closer to baking strains than the five strains in group I. These differences were reflected in their leavening ability in dough with and without addition of 5% sucrose, and their α-glucosidase activity, although most of the strains did not leaven dough with addition of 30% sucrose. Strain S-10 in group II showed a high rate of CO2 production from sponge dough without addition of sugar and consumed fermentable sugar... |
|
Palavras-chave: Brem; SUC2 gene; Leavening; Breadmaking; Dough. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3573 |
| |
| Registros recuperados: 37 | |
|
|
|
