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| CHAGAS,KRISTHIANO; CIPRIANO,JAMILE L.D.; LOPES,JOSÉ CARLOS; SCHMILDT,EDILSON R.; OTONI,WAGNER C.; ALEXANDRE,RODRIGO S.. |
| Abstract This study evaluated the effect of osmoregulators and carbohydrates on the maturation and germination of somatic embryos of papaya ‘Golden THB’. Cotyledon explants from papaya seedlings germinated in vitro on basal MS medium were cultured on somatic embryogenesis induction medium (IM) containing MS salts, myo-inositol, sucrose, agar and p-chlorophenoxyacetic acid. After 50 days, embryogenic calli were transferred onto maturation media (MM) for 45 additional days. For experiment 1, a MS-based medium supplemented with abscisic acid, activated charcoal and concentrations of PEG 6000 (0; 40; 50; 60 and 70 g L-1) was used, whereas for experiment 2 malt extract concentrations (0; 0.1; 0.2; 0.3 and 0.4 g L-1) were assessed. The normal cotyledonary... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carica papaya L; Malt extract; Myo-inositol; Polyethylene glycol. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000703433 |
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| CIPRIANO,JAMILE L.D.; CRUZ,ANA CLÁUDIA F.; MANCINI,KARINA C.; SCHMILDT,EDILSON R.; LOPES,JOSÉ CARLOS; OTONI,WAGNER C.; ALEXANDRE,RODRIGO S.. |
| ABSTRACT The aim of this study was to evaluate somatic embryogenesis in juvenile explants of the THB papaya cultivar. Apical shoots and cotyledonary leaves were inoculated in an induction medium composed of different concentrations of 2,4-D (6, 9, 12, 15 and 18 µM) or 4-CPA (19, 22, 25, 28 and 31 µM). The embryogenic calluses were transferred to a maturation medium for 30 days. Histological analysis were done during the induction and scanning electron microscopy after maturing. For both types of auxin, embryogenesis was achieved at higher frequencies with cotyledonary leaves incubated in induction medium than with apical shoots; except for callogenesis. The early-stage embryos (e.g., globular or heart-shape) predominated. Among the auxins, best results... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carica papaya L.; Juvenile explants; Maturation; Microscopic analysis; Synthetic auxins.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100385 |
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| GOUVEA,DRIELLY S.; CHAGAS,KRISTHIANO; CIPRIANO,JAMILE L.D.; LOPES,JOSÉ CARLOS; SCHMILDT,EDILSON R.; OTONI,WAGNER C.; SCHMILDT,OMAR; ARAUJO,CAROLINE P. DE; ALEXANDRE,RODRIGO S.. |
| Abstract: Somatic embryogenesis from explants from hermaphrodite papaya mother plants is an alternative for the production of true-to-type plants without the need for sexing. This study aimed to analyze hormonal and osmotic inducers in different somatic embryogenesis stages in the commercial hermaphrodite hybrid papaya UENF/Caliman 01. Leaf disks from in vitro shoots originated from ex vitro hermaphrodite plants were cultured in induction medium supplemented with different concentrations of 2,4-D (6, 9, 12, 15, and 18 μM) and 4-CPA (19, 22, 25, 28, and 31 μM). After 90 days, the formation of somatic embryos was verified. The 2,4-D induced the formation of light brown calli with low frequency (20%) of somatic embryogenesis. However, 4-CPA (25 μM) induced... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Abscisic acid; Auxins; Carica papaya L.; Gibberellic acid; Hermaphrodite explants; Polyethylene glycol. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500856 |
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| FRANÇA,JULIANY M.; VENIAL,LUCIMARA R.; COSTA,ELOÁ B.; SCHMILDT,EDILSON R.; SCHMILDT,OMAR; BERNARDES,PAULA M.; TATAGIBA,SANDRO D.; LOPES,JOSÉ C.; FERREIRA,MARCIA F.S.; ALEXANDRE,RODRIGO S.. |
| ABSTRACT Genetic diversity allows identification of potential intraspecific genotypes in the genus Passiflora. The objective of this study was to examine the morphological and genetic diversity of auxin-induced Passiflora mucronata. The experiments were arranged in a complete randomized block design, with a 9 x 2 factorial arrangement (nine genotypes x presence and absence of auxin, indole-3-butyric acid (IBA)), with four replicates of 16 cuttings. The rooting and vegetative growth responses were variable. Genotype 5 was more responsive in the absence of IBA and genotypes 3, 8 and 9 were more responsive in the presence of IBA. Auxin increased rooting rate and percentage, reducing the average time of root protrusion in eight days. IBA also contributed to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Indol-3-butyric acid; Rooting; Genotypes; Restinga passion fruit; Molecular markers ISSR; Propagation. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401799 |
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