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| REYES-ZAMBRANO,SHEILA JAZMÍN; LECONA-GUZMÁN,CARLOS ALBERTO; BARREDO-POOL,FELIPE ALONSO; AMBROSIO CALDERÓN,JOSÉ DOLORES; ABUD-ARCHILA,MIGUEL; RINCÓN-ROSALES,REINER; RUIZ-VALDIVIEZO,VÍCTOR MANUEL; GUTIÉRREZ-MICELI,FEDERICO ANTONIO. |
| Current protocols for Agave americana L. micropropagation have limited commercial application due to the low number of plants produced by explant. Indirect organogenesis could be an alternative, however is necessary to optimize plant growth regulators for plantlet number maximization. The objective of this work was to optimize 2,4-dicholorophenoxyacetic acid (2,4-D) and 6-benzyl adenine (BA) concentrations on the induction of A. americana embryogenic callus from apical meristem as explant for maximized the number of shoots per callus using a response surface experimental design. MS medium containing 30 g l-1 sucrose amended with 0.11,0.18, 0.45 or 2.26 μM 2,4-D, and 11.0, 22.0, 38.2 or 44.0 μM BA was used. Nine treatments with three... |
| Tipo: Journal article |
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| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432016000100014 |
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