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EFFECT OF GENOTYPE ON THE in vitro REGENERATION OF Stevia rebaudiana VIA SOMATIC EMBRYOGENESIS Acta biol.Colomb.
NARANJO,Esther Julia; FERNANDEZ BETIN,Osman; URREA TRUJILLO,Aura Inés; CALLEJAS POSADA,Ricardo; ATEHORTÚA GARCÉS,Lucía.
Stevia rebaudiana (Asteraceae) is a plant of economic importance because of its medicinal properties and the presence of sweetener compounds on its leaves. These compounds can be a substitute for sucrose in a wide variety of products used by persons with diabetes and obesity problems. To standardize an efficient and effective propagation method for the different Stevia genotypes grown in Colombia, this study evaluated the effect of different combinations of the plant growth regulators 2,4-dichlorophenoxyacetic acid (2,4-D), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), 6-(gamma, gamma-dimethylallylamino) purine (2iP) and Zeatin on the induction and development of somatic embryos. Adenine and coconut water were also evaluated as supplements in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: In vitro propagation natural sweetener somatic embryo 2; 4-dichlorophenoxyacetic acid (2; 4-D) 2-isopentenyl adenine (2iP).
Ano: 2016 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2016000100009
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POLYPHENOLS DISTRIBUTION AND RESERVE SUBSTANCES ANALYSIS IN CACAO SOMATIC EMBRYOGENESIS Acta biol.Colomb.
GALLEGO RÚA,Adriana María; HENAO RAMÍREZ,Ana María; URREA TRUJILLO,Aura Inés; ATEHORTÚA GARCÉS,Lucía.
In order to understand the causes of lack of regeneration in cacao somatic embryos, two cacao varieties with different responses to regeneration potential were described based on their capacity to store different compounds. It is well known that seed reserves play a central role in the regenerative capability of somatic embryos; thus, we followed histochemical changes and reserve fluctuations of proteins, polysaccharides and polyphenols during somatic embryogenesis (SE) in the two cacao varieties. The study showed that, in somatic embryos of the regenerating variety, polyphenols were localized mainly in the periphery of the embryo (epidermal cells) and proteins were the main storage substance in the embryo expression medium, while the non-regenerating...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidants; Histology; Reserve accumulation; Recalcitrance; Somatic embryogenesis.
Ano: 2016 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2016000200002
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IN VITRO GERMINATION AND VEGETATIVE PROPAGATION THROUGH BUD DEVELOPMENT OF SACHA INCHI (Plukenetia volubilis) Acta biol.Colomb.
HENAO RAMÍREZ,Ana María; URREA TRUJILLO,Aura Inés; ATEHORTÚA GARCÉS,Lucía.
ABSTRACT This study describes the in vitro seed germination and micropropagation of Plukenetia volubilis (sacha inchi), an oilseed crop rich in omega-3 fatty acids, with health benefits and several industrial applications. Seed germination was evaluated in different culture media (MS and 1/2 MS), seed coat presence/absence and culture temperature (18 °C and 28 °C). Micropropagation was performed using axillary bud development (ABD) on nodal segments from in vitro seedlings. KIN, BAP and 2-ip were evaluated for ABD, and the effect of modified MS in 453 mg L-1 CaCl2 and 351.62 mg L-1 MgSO4 on ABD and shoot survival was assessed to improve the process. Finally, six treatments were evaluated to optimize ABD and shoot leaf formation. Seed germination of 91.6 %...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Axillary buds; Calcium; Kinetin; Micropropagation; 2-ip.
Ano: 2022 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2022000100070
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