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| Tabatabaei,Meisam; Zakaria,Mohd Rafein; Rahim,Raha Abdul; Wright,André-Denis G.; Shirai,Yoshihito; Abdullah,Norhani; Sakai,Kenji; Ikeno,Shinya; Mori,Masatsugu; Kazunori,Nakamura; Sulaiman,Alawi; Hassan,Mohd Ali. |
| 16S ribosomal RNA (rRNA)-targeted fluorescent in situ hybridization combined with polymerase chain reaction (PCR)-cloning, light microscopy using Gram stains, scanning electron microscopy and denatured gradient gel electrophoresis were used to reveal the distribution of methanogens within an anaerobic closed digester tank fed with palm oil mill effluent. For specific detection of methanogens, 16S rRNA-cloning analysis was conducted followed by restriction fragment length polymorphism (RFLP) for presumptive identification of methanogens. To cover the drawbacks of the PCR-cloning study, the organization of the microorganisms was visualized in the activated sludge sample by using fluorescent oligonucleotide probes specific to several different methanogens,... |
| Tipo: Journal article |
Palavras-chave: Anaerobic digestion; DGGE; FISH; Methanogens; POME. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300012 |
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