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The occurrence of Aeromonas spp. in the bottled mineral water, well water and tap water from the municipal supplies BABT
Scoaris,Denise de Oliveira; Bizerra,Fernando Cezar; Yamada-Ogatta,Sueli Fumie; Abreu Filho,Benício Alves de; Ueda-Nakamura,Tânia; Nakamura,Celso Vataru; Dias Filho,Benedito Prado.
The aim of this work was to study the occurrence of Aeromonas sp in the bottled mineral water, well water and tap water from the municipal supplies. Positive samples were found for Aeromonas spp. 12.7% from the mineral water, 8.3% from the artesian water and 6.5% from the tap water. The recovery of Aeromonas spp. was significantly higher in the bottled mineral and artesian water than in the tap water from municipal supplies. The occurrence of the Aeromonas spp. did not correlate significantly with the contamination indicator bacteria (i.e. total coliforms) in the artesian water samples. However, a significant correlation was found between Aeromonas spp. and total coliforms in the both mineral water and tap water samples. The presence or absence of a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aeromonas; Drinking water; Indicator bacteria; Coliforms.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500022
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Antibacterial and antibiofilm activity of carvacrol against Salmonella enterica serotype Typhimurium BJPS
Trevisan,Daliah Alves Coelho; Silva,Alex Fiori da; Negri,Melyssa; Abreu Filho,Benício Alves de; Machinski Junior,Miguel; Patussi,Eliana Valéria; Campanerut-Sá,Paula Aline Zanetti; Mikcha,Jane Martha Graton.
ABSTRACT The present study evaluated the antibacterial and antibiofilm activity of carvacrol against Salmonella Typhimurium. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined and the time-kill curve and scanning electron microscopy (SEM) were performed to evaluate antibacterial activity. Antibiofilm activity was evaluated by quantifying total biomass using crystal violet assay, and metabolic activity was determined using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. The action of carvacrol against preformed biofilm on polypropylene and stainless steel was also evaluated by colony counting and SEM. The MIC and MBC was 312 µg mL-1. Carvacrol at MIC and 2 x MIC eliminated cells...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carvacrol; Biofilm; Antibacterial activity; Salmonella Typhimurium..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100612
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Contribution of environmental factors in the formation of biofilms by Alicyclobacillus acidoterrestris on surfaces of the orange juice industry Ciência Rural
Dutra,Tatiane Viana; Prado,Daniela Biral do; Anjos,Márcia Maria dos; Machinski Junior,Miguel; Mikcha,Jane Martha Graton; Abreu Filho,Benício Alves de.
ABSTRACT: The objective of this study was to evaluate the effect of the initial microbial load, temperature and contact time on the biofilm formation of Alicyclobacillus acidoterrestris on stainless steel and natural food-grade rubber using orange juice as culture medium. The low initial load of A. acidoterrestris (2 log CFU/mL) led to biofilm formation on the stainless steel surface after 48 h of contact at 28 ºC and after 24 h at 45 ºC, and on natural food-grade rubber surface after 48 h of contact at both temperatures. The high initial microbial load (5 log CFU/mL) led to biofilm formation on stainless steel after 4 h of contact at 28 °C and 45 °C, while biofilm was formed on natural food-grade rubber after 8 h of contact at 28 °C and 4 h at 45 °C. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Concentrated orange juice; Stainless steel; Natural food-grade rubber; Spores; Biofilm.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000300753
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