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Isolation and biological characterization of a basic phospholipase A2 from Bothrops jararacussu snake venom Biocell
Maruñak,S.L.; Leiva,L; Garcia Denegri,M.E.; Teibler,P; Acosta De Pérez,O.
A phospholipase A2 has been isolated from Bothrops jararacussu venom from snakes that inhabit the northeast region of Argentina. The present study describes in vivo and in vitro biological activities of phospholipase A2 from B. jararacussu as well as isolation details of its. Venom was obtained by milking of adult snakes which were housing in wood reptile cages of varying dimensions in heated (20-30ºC) rooms. Snakes received a weekly diet of mice and water was available ad libitum for drinking and soaking. The enzyme was purified by gel filtration on a Sephadex G-75 column followed by ion exchange chromatography on a SP-Sephadex C25 column. The major peak belonging to proteins was retained in the cation exchanger and then eluted using a concentration...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Snake venom; Bothrops jararacussu; Phospholipase A2; Isolation.
Ano: 2007 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300001
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