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	Provedor de dados Electron. J. Biotechnol. (1) Autor Acquaah,George (1) Kebede,Bizuayehu (1) Matand,Kanyand (1) Williams,Sonya (1) Wu,Ning (1) Palavra-chave Cell competency (1) DNA (1) E. coli DH10B (1) Electroporation (1) Tipo do documento Journal article (1) Ano 2010 (1) País Chile (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Enhancing DNA electrotransformation efficiency in Escherichia coli DH10B electrocompetent cells Electron. J. Biotechnol. Wu,Ning; Matand,Kanyand; Kebede,Bizuayehu; Acquaah,George; Williams,Sonya. Electrotransformation also known as electroporation is the most reliable and efficient tool for plasmid DNA uptake. Electrotransformation efficiency is function of many factors which include (1) number of cell washes prior to electroporation, (2) electroporation cell number, (3) electroporation DNA amount, and (4) cell growth phase. Those factors have limitedly been concomitantly investigated in E. coli DH10B strain. This study is aimed to explore above key factors to define the optimal conditions for high electrotransformation efficiency. The results showed that electrotransformation efficiency of E. coli DH10B was enhanced to 1.5 x 10(9) cfu/µg by washing cells three times with 15 ml of 10% glycerol. This washed off extra salts from cell suspension and... Tipo: Journal article Palavras-chave: Cell competency; DNA; E. coli DH10B; Electroporation. Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500021 Registros recuperados: 1 Primeira ... 1 ... Última

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