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Clostridium perfringens isolate typing by multiplex PCR J. Venom. Anim. Toxins incl. Trop. Dis.
Ahsani,MR; Mohammadabadi,MR; Shamsaddini,MB.
Clostridium perfringens is an important pathogen that provokes numerous different diseases. This bacterium is classified into five different types, each of which capable of causing a different disease. There are various methods for the bacterial identification, many are labor-intensive, time-consuming, expensive and also present low sensitivity and specificity. The aim of this research was to identify the different types of C. perfringens using PCR molecular method. In this study, 130 sheep-dung samples were randomly collected from areas around the city of Kerman, southeastern Iran. After processing and culturing of samples, the produced colonies were morphologically studied, gram stain test was also carried out and the genera of these bacteria were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clostridium perfringens; Multiplex PCR; Biochemical tests; Genetic typing.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400006
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