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Molecular assessment of Gymnotus spp. (Gymnotiformes: Gymnotidae) fishing used as live baitfish in the Tietê River, Brazil Neotropical Ichthyology
Faria-Pereira,Lilian Paula; Hilsdorf,Alexandre Wagner Silva; Albert,James; Paiva,Maria José Tavares Ranzani; Galvão,Márcia Santos Nunes.
ABSTRACT The capture of live bait for sport fishing is an important activity for fishing communities. The main species used for this purpose are members of the genus Gymnotus, which comprises numerous species of cryptic nature that are difficult to identify based on external morphology. The aims of this work were to identify through partial sequences of the COI gene Gymnotus species fished in the Jacaré-Guaçu River, SP, and to develop a molecular diagnostic approach using PCR-RFLP to identify these species. Partial COI sequences were compared to those of other species deposited in GenBank. The sequences were assessed in the NEBCutter program to determine restriction sites in the sequence and the enzymes to be tested. Phenetic analysis performed by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular identification; PCR-RFLP; Tuvira.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000500205
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