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Population-specific variations of the genetic architecture of sex determination in wild European sea bass Dicentrarchus labrax L. ArchiMer
Faggion, Sara; Vandeputte, Marc; Chatain, Beatrice; Gagnaire, Pierre-alexandre; Allal, Francois.
Polygenic sex determination (PSD) may show variations in terms of genetic and environmental components between populations of fish species exposed/adapted to different environments. The European sea bass (Dicentrarchus labrax) is an interesting model, combining both a PSD system and a genetic subdivision into an Atlantic and a Mediterranean lineage, with genetic substructures within the Mediterranean Sea. Here, we produced experimental progeny crosses (N = 927) from broodstock sampled in four wild populations (North Atlantic, NAT; Western Mediterranean, WEM; North-Eastern Mediterranean, NEM; South-Eastern Mediterranean, SEM). We found less females than males in the progeny, both in the global dataset (32.5%) and within each paternal group (from 25.1% for...
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Ano: 2019 URL: https://archimer.ifremer.fr/doc/00464/57609/59864.pdf
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How to genetically increase fillet yield in fish: Relevant genetic parameters and methods to predict genetic gain ArchiMer
Vandeputte, Marc; Fraslin, Clemence; Haffray, Pierrick; Bestin, Anastasia; Allal, Francois; Kocour, Martin; Prchal, Martin; Dupont-nivet, Mathilde.
Fillet yield (i.e. the proportion of edible muscle in a fish) is a key economic trait for species sold as fillets. Its genetic improvement is complicated by several of its characteristics 1) it is a ratio trait, 2) its numerator (fillet weight) and denominator (body weight) are strongly correlated (correlations in the range 0.89-0.99), 3) it offers little phenotypic variation and 4) it cannot be measured on alive breeding candidates. In a former study, we showed that it could be improved by selection, especially with three selection indices, fillet yield, residual fillet weight and a ratio-specific linear index. However, it is well known that the heritability of ratio traits does not permit a reliable prediction of genetic gains. As predictability of...
Tipo: Text Palavras-chave: Aquaculture; Genetics; Heritability; Selection; Linear index; Residual fillet weight; Selection response; Simulation; Processing yields.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00612/72378/71390.pdf
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Estimates of genetic variability and inbreeding in experimentally selected populations of European sea bass ArchiMer
Hillen, J. E. J.; Coscia, I.; Vandeputte, Marc; Herten, K.; Hellemans, B.; Maroso, F.; Vergnet, Alain; Allal, Francois; Maes, G. E.; Volckaert, F. A. M..
The aquaculture industry has increasingly aimed at improving economically important traits like growth, feed efficiency and resistance to infections. Artificial selection represents an important window of opportunity to significantly improve production. However, the pitfall is that selection will reduce genetic diversity and increase inbreeding in the farmed stocks. Genetic tools are very useful in this context as they provide accurate measures of genetic diversity together with many additional insights in the stock status and the selection process. In this study we assessed the level of genetic variability and relatedness over several generations of two lines of experimentally selected European sea bass (Dicentrarchus labrax L.). The first line was...
Tipo: Text Palavras-chave: Artificial selection; DdRAD; Fish; Genetic diversity; Genomics; Inbreeding.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00392/50314/50993.pdf
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Case report of osteomyelitis of the mandible in osteopetrosis and management considerations ArchiMer
Allal, Sarah; Rabuel, Valentin; Gengler, Charline; Douchet, Catherine; Allal, Francois; Zwetyenga, Narcisse.
Introduction and importance Osteopetrosis is a poorly known and probably underdiagnosed pathology. It is caused by various genetic abnormalities resulting in osteoclast dysfunction. Functional and aesthetic consequences have a major impact on the patient’s quality of life. Ten percent of osteopetrosis cases develop osteomyelitis that usually involves the mandible. Management of this complication remains complex and often unsatisfactory. Case presentation We report a case of a 62-year-old woman with osteopetrosis, complicated by mandibular osteomyelitis with intra-oral bone exposure and submental fistulas. Management was performed with antibiotic therapy and surgical necrotic resection. This cured the fistulas but the bone exposure persisted. Discussion...
Tipo: Text Palavras-chave: Osteomyelitis; Mandible; Case report; Osteopetrosis; Management.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00686/79816/82611.pdf
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Combining vitassign and colony: an efficient practical procedure for parental assignment with missing parents ArchiMer
Doan, Q.k.; Vandeputte, Marc; Chatain, Beatrice; Vergnet, Alain; Allal, Francois.
Tipo: Text
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00281/39196/37750.pdf
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Heritability of Boldness and Hypoxia Avoidance in European Seabass, Dicentrarchus labrax ArchiMer
Ferrari, Sebastien; Horri, Khaled; Allal, Francois; Vergnet, Alain; Benhaim, David; Vandeputte, Marc; Chatain, Beatrice; Begout, Marie-laure.
To understand the genetic basis of coping style in European seabass, fish from a full factorial mating (10 females x 50 males) were reared in common garden and individually tagged. Individuals coping style was characterized through behavior tests at four different ages, categorizing fish into proactive or reactive: a hypoxia avoidance test (at 255 days post hatching, dph) and 3 risk-taking tests (at 276, 286 and 304 dph). We observed significant heritability of the coping style, higher for the average of risk-taking scores (h2 = 0.45 ± 0.14) than for the hypoxia avoidance test (h2 = 0.19 ± 0.10). The genetic correlations between the three risk-taking scores were very high (rA = 0.96–0.99) showing that although their repeatability was moderately high (rP =...
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Ano: 2016 URL: https://archimer.ifremer.fr/doc/00362/47278/47239.pdf
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Contribution of a bioenergetics model to investigate the growth and survival of European seabass in the Bay of Biscay – English Channel area ArchiMer
Dambrine, Chloe; Huret, Martin; Woillez, Mathieu; Pecquerie, Laure; Allal, Francois; Servili, Arianna; De Pontual, Helene.
The European seabass (Dicentrarchus labrax) is a species of particular ecological and economic importance. Stock assessments have recently revealed the worrying state of the “Northern stock”, probably due to overfishing and a series of poor recruitments. The extent to which these poor recruitments are due to environmental variability is difficult to assess, as the processes driving the seabass life cycle are poorly known. Here we investigate how food availability and temperature may affect the growth and survival of wild seabass at the individual scale. To this end, we developed a bioenergetics model based on the Dynamic Energy Budget (DEB) theory. We applied it to seabass population of the Northeast Atlantic region (Bay of Biscay – English Channel area)...
Tipo: Text Palavras-chave: Dicentrarchus labrax Northeast Atlantic Dynamic Energy Budget theory Growth; Starvation Early-life stages.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00612/72397/71341.pdf
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Evaluation of a European sea bass (Dicentrarchus labrax L.) post-larval tagging method with ultra-small RFID tags ArchiMer
Faggion, Sara; Sanchez, Pierre; Vandeputte, Marc; Clota, Frederic; Vergnet, Alain; Blanc, Marie-odile; Allal, Francois.
Individual tagging is key to a better understanding of early life stages in fish. Very small RFID transponder microchips (500 × 500 × 100 μm, 82 μg) are now available. The aim of this study was to develop a protocol to tag European sea bass (Dicentrarchus labrax L.) larvae from 61 days post-hatching (dph; standard length ~10 mm) to 96 dph (standard length ~28 mm) through intra-coelomic implantation of microchips. The suitability of such a tagging procedure was evaluated, with the purpose of determining the minimal fish age and body size for microchip tagging without adverse effects on survival and growth performance. We produced an experimental population composed by 50:50 normally pigmented larvae and albino larvae through artificial fertilization. Five...
Tipo: Text Palavras-chave: Larvae; RFID transponder; Individual identification; Tagging effects; Dicentrarchus labrax L.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00601/71331/73084.pdf
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The European sea bass: a key marine fish model in the wild and in aquaculture ArchiMer
Vandeputte, Marc; Gagnaire, P.‐a.; Allal, Francois.
The European sea bass (Dicentrarchus labrax L.) is a marine fish of key economic and cultural importance in Europe. It is now more an aquaculture than a fisheries species (>96% of the production in 2016), although modern rearing techniques date back only from the late 1980s. It also has high interest for evolutionary studies, as it is composed of two semispecies (Atlantic and Mediterranean lineages) that have come into secondary contact following the last glaciation. Based on quantitative genetics studies of most traits of interest over the past 10–15 years, selective breeding programs are now applied to this species, which is at the beginning of its domestication process. The availability of a good quality reference genome has accelerated the...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Genetics; Heritability; Population genetics.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00486/59752/62858.pdf
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The origin and remolding of genomic islands of differentiation in the European sea bass ArchiMer
Duranton, Maud; Allal, Francois; Fraisse, Christelle; Bierne, Nicolas; Bonhomme, Francois; Gagnaire, Pierre-alexandre.
Speciation is a complex process that leads to the progressive establishment of reproductive isolation barriers between diverging populations. Genome-wide comparisons between closely related species have revealed the existence of heterogeneous divergence patterns, dominated by genomic islands of increased divergence supposed to contain reproductive isolation loci. However, this divergence landscape only provides a static picture of the dynamic process of speciation, during which confounding mechanisms unrelated to speciation can interfere. Here we use haplotype-resolved whole-genome sequences to identify the mechanisms responsible for the formation of genomic islands between Atlantic and Mediterranean sea bass lineages. Local ancestry patterns show that...
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00446/55750/57409.pdf
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The contribution of ancient admixture to reproductive isolation between European sea bass lineages ArchiMer
Duranton, Maud; Allal, Francois; Valiere, Sophie; Bouchez, Olivier; Bonhomme, Francois; Gagnaire, Pierre-alexandre.
Understanding how new species arise through the progressive establishment of reproductive isolation (RI) barriers between diverging populations is a major goal in Evolutionary Biology. An important result of speciation genomics studies is that genomic regions involved in RI frequently harbor anciently diverged haplotypes that predate the reconstructed history of species divergence. The possible origins of these old alleles remain much debated, as they relate to contrasting mechanisms of speciation that are not yet fully understood. In the European sea bass (Dicentrarchus labrax), the genomic regions involved in RI between Atlantic and Mediterranean lineages are enriched for anciently diverged alleles of unknown origin. Here, we used haplotype-resolved...
Tipo: Text Palavras-chave: Ancient admixture; Introgression; Reproductive isolation; Genomic conflicts; Marine fish.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00636/74768/74920.pdf
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Combining vitassign and colony for pedigree reconstruction in a case of factorial mating with missing parental genotypes ArchiMer
Allal, Francois; Doan, Q.k.; Chatain, Beatrice; Vergnet, Alain; Vandeputte, Marc.
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Ano: 2015 URL: http://archimer.ifremer.fr/doc/00306/41694/40971.pdf
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How could fully scaled carps appear in natural waters in Madagascar? ArchiMer
Hubert, Jean-noel; Allal, Francois; Hervet, Caroline; Ravakarivelo, Monique; Jeney, Zsigmond; Vergnet, Alain; Guyomard, Rene; Vandeputte, Marc.
The capacity of organisms to rapidly evolve in response to environmental changes is a key feature of evolution, and studying mutation compensation is a way to evaluate whether alternative routes of evolution are possible or not. Common carps (Cyprinus carpio) carrying a homozygous loss-of-function mutation for the scale cover gene fgfr1a1, causing the 'mirror' reduced scale cover, were introduced in Madagascar a century ago. Here we show that carps in Malagasy natural waters are now predominantly covered with scales, though they still all carry the homozygous mutation. We also reveal that the number of scales in mutated carps is under strong polygenic genetic control, with a heritability of 0.49. As a whole, our results suggest that carps submitted to...
Tipo: Text Palavras-chave: Evolution; Mutation compensation; Heritability.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00356/46681/46725.pdf
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Combining Individual Phenotypes of Feed Intake With Genomic Data to Improve Feed Efficiency in Sea Bass ArchiMer
Besson, Mathieu; Allal, Francois; Chatain, Beatrice; Vergnet, Alain; Clota, Frederic; Vandeputte, Marc.
Measuring individual feed intake of fish in farms is complex and precludes selective breeding for feed conversion ratio (FCR). Here, we estimated the individual FCR of 588 sea bass using individual rearing under restricted feeding. These fish were also phenotyped for their weight loss at fasting and muscle fat content that were possibly linked to FCR. The 588 fish were derived from a full factorial mating between parental lines divergently selected for high (F+) or low (F–) weight loss at fasting. The pedigree was known back to the great grand-parents. A subset of 400 offspring and their ancestors were genotyped for 1,110 SNPs which allowed to calculate the genomic heritability of traits. Individual FCR and growth rate in aquarium were both heritable...
Tipo: Text Palavras-chave: Aquaculture; Feed conversion ratio; Fine phenotyping; Genomic selection; Individual feed intake; Restricted feeding; Selective breeding.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00486/59799/62927.pdf
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Genetic variation of resistance to Viral Nervous Necrosis and genetic correlations with production traits in wild populations of the European sea bass ( Dicentrarchus labrax ) ArchiMer
Khanh, Doan Quoc; Vandeputte, Marc; Chatain, Beatrice; Haffray, Pierrick; Vergnet, Alain; Breuil, Gilles; Allal, Francois.
Viral Nervous Necrosis (VNN) disease is considered as one of the most serious threats for European sea bass cultured in Mediterranean Sea, with no simple and effective procedures to treat this disease. In this study, 1472 offspring resulting from artificial full factorial mating of western Mediterranean dams with sires from four different wild populations of European sea bass (Northern Atlantic, NAT; Western Mediterranean, WEM; Northern-East Mediterranean, NEM; and Southern-East Mediterranean, SEM) were challenged by experimental infection to W80 betanodavirus strain in order to evaluate genetic variations for VNN resistance among populations and genetic correlations between VNN resistance and production traits. The results showed a large variation of VNN...
Tipo: Text Palavras-chave: VNN disease; VNN resistance; Heritability; Genetic correlation.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00385/49612/50124.pdf
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APIS: An Auto‐Adaptive Parentage Inference Software that tolerates missing parents ArchiMer
Griot, Ronan; Allal, Francois; Brard‐fudulea, S; Morvezen, R; Haffray, P; Phocas, F; Vandeputte, Marc.
In the context of parentage assignment using genomic markers, key issues are genotyping errors and an absence of parent genotypes because of sampling, traceability or genotyping problems. Most likelihood‐based parentage assignment software programs require a priori estimates of genotyping errors and the proportion of missing parents to set up meaningful assignment decision rules. We present here the R package APIS, which can assign offspring to their parents without any prior information other than the offspring and parental genotypes, and a user‐defined, acceptable error rate among assigned offspring. Assignment decision rules use the distributions of average Mendelian transmission probabilities, which enable estimates of the proportion of offspring with...
Tipo: Text Palavras-chave: Microsatellites; Missing parents; Parentage assignment; Pedigree; SNP.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00587/69866/67767.pdf
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Improving feed efficiency in fish using selective breeding: a review ArchiMer
De Verdal, Hugues; Komen, Hans; Quillet, Edwige; Chatain, Beatrice; Allal, Francois; Benzie, John A. H.; Vandeputte, Marc.
Improving feed efficiency (FE) is key to reducing production costs in aquaculture and to achieving sustainability for the aquaculture industry. Feed costs account for 30–70% of total production costs in aquaculture; much work has been done on nutritional and husbandry approaches to improve FE but only a limited amount of research has been devoted to using genetics, despite its potential. This paper reviews past work to improve FE in fish using selective breeding and assess future directions. Direct selection on FE traits requires methods to measure individual feed consumption and estimate FE efficiently and accurately. This is particularly difficult to do in fish because of the environment in which they live. Many of the published studies on FE were found...
Tipo: Text Palavras-chave: Feed conversion ratio; Feed efficiency; Feed intake; Fish; Genetics; Selection.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00415/52675/53771.pdf
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Viral encephalopathy and retinopathy in aquaculture: a review ArchiMer
Doan, Q. K.; Vandeputte, Marc; Chatain, Beatrice; Morin, T; Allal, Francois.
Viral encephalopathy and retinopathy (VER), otherwise known as viral nervous necrosis (VNN), is a major devastating threat for aquatic animals. Betanodaviruses have been isolated in at least 70 aquatic animal species in marine and in freshwater environments throughout the world, with the notable exception of South America. In this review, the main features of betanodavirus, including its diversity, its distribution and its transmission modes in fish, are firstly presented. Then, the existing diagnosis and detection methods, as well as the different control procedures of this disease, are reviewed. Finally, the potential of selective breeding, including both conventional and genomic selection, as an opportunity to obtain resistant commercial populations, is...
Tipo: Text Palavras-chave: Betanodavirus; Disease resistance; Genetics; Nervous necrosis virus; Selective breeding.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00350/46132/46024.pdf
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Combining Vitassign and Colony for pedigree reconstruction in a case of factorial mating with missing parental genotypes ArchiMer
Allal, Francois; Doan, Q.k.; Chatain, Beatrice; Vergnet, Alain; Vandeputte, Marc.
Tipo: Text
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00281/39180/37742.pdf
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Genome-wide association and genomic prediction of resistance to viral nervous necrosis in European sea bass (Dicentrarchus labrax) using RAD sequencing ArchiMer
Palaiokostas, Christos; Cariou, Sophie; Bestin, Anastasia; Bruant, Jean-sebastien; Haffray, Pierrick; Morin, Thierry; Cabon, Joelle; Allal, Francois; Vandeputte, Marc; Houston, Ross D..
Background: European sea bass (Dicentrarchus labrax) is one of the most important species for European aquaculture. Viral nervous necrosis (VNN), commonly caused by the redspotted grouper nervous necrosis virus (RGNNV), can result in high levels of morbidity and mortality, mainly during the larval and juvenile stages of cultured sea bass. In the absence of efficient therapeutic treatments, selective breeding for host resistance offers a promising strategy to control this disease. Our study aimed at investigating genetic resistance to VNN and genomic-based approaches to improve disease resistance by selective breeding. A population of 1538 sea bass juveniles from a factorial cross between 48 sires and 17 dams was challenged with RGNNV with mortalities and...
Tipo: Text
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00445/55676/57357.pdf
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