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Innovative metabolomic workflows to study growth kinetics dinoflagellate cysts revived from modern and ancient sediments ArchiMer
Mondeguer, Florence; Souard, Florence; Guitton, Yann; Allard, Pierre-marie; Elie, Nicolas; Sibat, Manoella; Latimier, Marie; Quere, Julien; Wolfender, Jean-luc; Siano, Raffaele; Hess, Philipp.
Aquatic ecosystems worldwide have been negatively affected by eutrophication, many of them driven by increasing nutrient inputs from untreated domestic sewage and industrial and agricultural wastewater. During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in the sediments of the coastal marine environment for hundreds of years. As a working hypothesis, we presumed that these two genera have preserved their adaptation to such ecosystem changes in the form of cysts in the old sediments. From revivified cysts, some cultures of toxic and non-toxic dinoflagellates (A. minutum and S. donghaienis) were analyzed by a metabolomic approach to study the possible adaptive responses of...
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00464/57591/59769.pdf
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Workflows novateurs pour une analyse métabolomique plus claire et plus intégrative ArchiMer
Mondeguer, Florence; Allard, Pierre-marie; Souard, Florence; Elie, Nicolas; Guitton, Yann; Sibat, Manoella; Siano, Raffaele.
During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in marine sediments for hundreds of years. Our working hypothesis assumes that these two genera have "recorded" their adaptation to ecosystem changes in these cells preserved in the form of cysts. From these revived cysts, toxic and non-toxic dinoflagellate cultures (Alexandrium minutum and Scrippsiella donghaienis) were analyzed by a metabolomic approach to investigate the possible adaptive responses of phytoplankton to these changes in coastal ecosystems. The strains were obtained from isotopically dated sediments, 1986 and 1996 for "old" and 2006 for "modern". In total, 84 samples were obtained. Each experimental...
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Ano: 2019 URL: https://archimer.ifremer.fr/doc/00485/59662/62721.pdf
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Sélection de workflows adaptés à une analyse métabolomique plus transparente et intégrative ArchiMer
Mondeguer, Florence; Allard, Pierre-marie; Souard, Florence; Elie, Nicolas; Guitton, Yann; Sibat, Manoella; Siano, Raffaele.
During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in marine sediments for hundreds of years. Our working hypothesis assumes that these two genera have "recorded" their adaptation to ecosystem changes in these cells preserved in the form of cysts. From these revived cysts, toxic and non-toxic dinoflagellate cultures (Alexandrium minutum and Scrippsiella donghaienis) were analyzed by a metabolomic approach to investigate the possible adaptive responses of phytoplankton to these changes in coastal ecosystems. The strains were obtained from isotopically dated sediments, 1986 and 1996 for "ancient" and 2006 for "modern". In total, 84 samples were obtained. Each experimental...
Tipo: Text
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00498/60941/64339.pdf
Registros recuperados: 3
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