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| Vasconcelos,Maria José V. de; Abdelnoor,Ricardo V.; Karan,Décio; Almeida,Álvaro M. R.; Oliveira,Maurílio F. de; Barros,Everaldo G.; Moreira,Maurílio A.. |
| Euphorbia heterophylla, também conhecida como amendoim-bravo ou leiteira, é considerada planta invasora importante em mais de 56 países, inclusive no Brasil, tendo acarretado perdas de até 33 % na cultura da soja. Fenotipicamente, é uma espécie de características variáveis, especialmente em relação ao formato do limbo foliar. Esta variabilidade fenotípica tem sido utilizada para diferenciar e classificar as plantas, sugerindo a vários autores que a leiteira seria, de fato, constituída por diferentes espécies. Para estudar a variabilidade genética a nível de DNA entre plantas de Euphorbia heterophylla, que apresentam folhas morfologicamente diferentes, foram analisadas dez plantas diferentes coletadas em campos de soja, em Londrina/PR. As plantas foram... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Marcadores moleculares; Diversidade genética; Euphorbia heterophylla. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582000000200010 |
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| Almeida,Álvaro M. R.; Piuga,Fernanda F.; Marin,Silvana R. R.; Binneck,Eliseu; Sartori,Fábio; Costamilan,Leila M.; Teixeira,Maria R. O.; Lopes,Marcelo. |
| Cercospora kikuchii, involved with the defoliation of soybean (Glycine max) plants, is normally associated with Septoria glycines in late season. Seventy-two isolates from different regions in Brazil, obtained mainly from purple stained seeds, showed phenotypic variation. Cercosporin content and rate of colony growth was higly variable among isolates. A strong correlation between cercosporin content and virulence was identified. Genetic variation among and within populations was evaluated based on 86 RAPD loci. The RAPD analysis clustered all isolates into seven groups. No relationship was observed between RAPD groups and geographic origin or cercosporin content. The sequence of the internal spacer regions (ITS1-5.8S-ITS2) from 13 isolates chosen according... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genotypic diversity; PCR-RFLP; RAPD; ITS sequence; Virulence. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000600005 |
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| Almeida,Álvaro M. R.; Amorim,Lilian; Bergamin Filho,Armando; Torres,Eleno; Farias,José R. B.; Benato,Luís C.; Pinto,Mauro C.; Valentim,Nilson. |
| The increase in incidence of charcoal rot caused by Macrophomina phaseolina on soybeans (Glycine max) was followed four seasons in conventional and no-till cropping systems. In the 1997/98 and 2000/01 seasons, total precipitation between sowing and harvest reached 876.3 and 846.9 mm, respectively. For these seasons, disease incidence did not differ significantly between the no-till and conventional systems. In 1998/99 and 1999/00 precipitation totaled 689.9 and 478.3 mm, respectively. In 1998/99, in the no-till system, the disease incidence was 43.7% and 53.1% in the conventional system. In 1999/00 the final incidence was 68.7% and 81.2% for the no-till and conventional systems, respectively. For these two seasons, precipitation was lower than that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Macrophomina phaseolina; Disease progress curves; Microsclerotia. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582003000200002 |
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| Gasparin,Marcos D. G.; Bianchini,Anésio; Souto,Eliezer R. de; Gaspar,José Osmar; Almeida,Álvaro M. R.; Kitajima,Elliot W.. |
| Em lavouras de feijoeiro (Phaseolus vulgaris) da cultivar Carioca Comum, no município de Londrina, Estado do Paraná, foram encontradas plantas com sintomas de necrose da haste, mosaico clorótico leve e porte reduzido, semelhantes aos sintomas causados por infecção viral. Exames de microscopia eletrônica revelaram a presença de partículas isométricas. Em testes de imunodifusão dupla em gel de ágar os extratos foliares de plantas infetadas reagiram positivamente com anti-soro específico para o Southern bean mosaic virus (SBMV). O vírus foi purificado e a massa molecular de sua proteína capsidial foi estimada em 30 kDa, valor esperado para proteínas do capsídeo de vírus do gênero Sobemovirus. A gama de hospedeiras do SBMV isolado no Paraná foi restrita ao... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: SBMV; Sobemovirus; BRMV. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000100013 |
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| Almeida,Álvaro M. R.; Sakai,Junichi; Hanada,Kaoru; Oliveira,Thalita G.; Belintani,Priscila; Kitajima,Elliot W.; Souto,Eliezer R.; Novaes,Tanara G. de; Nora,Paulo S.. |
| A virus was isolated from soybean (Glycine max) plants with symptoms of dwarfing and bud blight in Wenceslau Braz County, Paraná, Brazil. The host range and properties resembled those of Tobacco streak virus (TSV). The purified virus showed three peaks in a frozen sucrose gradient. Antiserum was produced and the virus was serologically related to TSV. Electron microscopy detected 28 nm spherical particles. Coat protein (CP) had a Mr of 29.880 Da. A fragment of 1028 nt was amplified, cloned and sequenced. One open reading frame with 717 nt was identified and associated to the CP. The CP gene shared 83% identity with the sequence of TSV CP from white clover (Trifolium repens) (GenBank CAA25133). This is the first report of the biological and molecular... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nucleotide sequence; Elisa; Host range. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400005 |
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| Almeida,Álvaro M. R.; Abdelnoor,Ricardo V.; Arias,Carlos A. Arrabal; Carvalho,Valdemar P.; Jacoud Filho,David S.; Marin,Silvana R. R.; Benato,Luís C.; Pinto,Mauro C.; Carvalho,Cláudio G. P.. |
| Macrophomina phaseolina has been considered one of the most prevalent soybean (Glycine max) pathogens in Brazil. No genetic resistance has been determined in soybean and very little is known about the genetic diversity of this pathogen in tropical and sub-tropical regions. Fifty-five isolates from soybean roots were collected in different regions and analyzed through RAPD for genetic diversity. The UPGMA cluster analysis for 74 loci scored permitted identification of three divergent groups with an average similarity of 99%, 92% and 88%, respectively. The three groups corresponded to 5.45%, 59.95% and 34.6%, respectively of all isolates used. A single plant had three different haplotypes, while 10.9% of the analyzed plants had two different haplotypes. In... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RAPD; ITS; PCR-RFLP; Soybean charcoal rot. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582003000300009 |
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| Almeida,Álvaro M. R.; Piuga,Fernanda F.; Marin,Silvana R.R.; Kitajima,Elliot W.; Gaspar,José O.; Oliveira,Thalita G. de; Moraes,Tanara G. de. |
| Stunting and stem necrosis were noticed in soybeans (Glycine max) grown in 2000/2001 in West Central Brazil the same condition was also observed in the following year in plantations as far as 2,000 km from the initial area. Based on transmission (mechanical, graft, insect vector), purification and serology, electron microscopy and molecular studies the causal agent was determined to be a whitefly-borne carlavirus which is possibly related to Cowpea mild mottle virus (CpMMV). |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cowpea mild mottle virus; Whitefly; Bemisia tabaci biotype B.. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000200016 |
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| Silva,Marcia F.; Almeida,Álvaro M. R.; Arias,Carlos A. A.. |
| Este estudo objetivou determinar os efeitos, em duas cultivares de soja (Glycine max), causados por duas estirpes do Soybean mosaic virus (SMV) e da idade das plantas na inoculação em relação a parâmetros ligados ao rendimento. Dois experimentos foram conduzidos em casa de vegetação, nos quais as cultivares BRS 183 e UFV-16 Capinópolis foram inoculadas, mecânica e isoladamente, com as estirpes G1 e G5 do SMV, em três diferentes idades após a emergência. Os experimentos foram instalados segundo um delineamento inteiramente casualizado com cinco repetições. Foram avaliados diferentes características relacionadas à produção e qualidade de grãos e constatou-se que: (i) as duas estirpes causam danos sobre diferentes características ligadas ao rendimento e... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: SMV; Glycine max; Rendimento; Mancha. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582003000600003 |
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