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	Provedor de dados BJMBR (3) Autor Almeida,D.F. (3) Ferreira,L.C.S. (3) Alves,A.M.B. (2) Guillobel,H.C.R. (2) Lásaro,M.O. (2) Camacho,E.F. (1) Luna,M.G. (1) Mais... Palavra-chave CFA/I fimbriae (2) ETEC (2) Attenuated vaccines (1) Colonization factor antigen I (1) DNA vaccine (1) DNA vaccines (1) Diarrheal disease (1) Enterotoxigenic Escherichia coli (1) Salmonella bivalent vaccine (1) Salmonella typhimurium aroA (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 1999 (2) 1998 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Immunization against the colonization factor antigen I of enterotoxigenic Escherichia coli by administration of a bivalent Salmonella typhimurium aroA strain BJMBR Guillobel,H.C.R.; Luna,M.G.; Camacho,E.F.; Almeida,D.F.; Ferreira,L.C.S.. An expression plasmid (pCFA-1) carrying the cfaB gene that codes for the enterotoxigenic Escherichia coli (ETEC) fimbrial adhesin colonization factor antigen I (CFA/I) subunit was constructed and used to transform a derivative of the attenuated Salmonella typhimurium aroA vaccine strain SL3261 carrying an F'lacIq. Treatment of the transformed strain with isopropyl-ß-D-thiogalactopyranoside (IPTG) resulted in elevated in vitro expression of the CFA/I subunit. Although flagellar function and lipopolysaccharide (LPS) synthesis were similar in both the parental and the recombinant strains, spleen colonization was reduced in the recombinant strain. All BALB/c mice parenterally inoculated with the recombinant strain developed significant anti-CFA/I and anti-LPS... Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella typhimurium aroA; Enterotoxigenic Escherichia coli; Attenuated vaccines; Colonization factor antigen I. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400012 New vaccine strategies against enterotoxigenic Escherichia coli: I: DNA vaccines against the CFA/I fimbrial adhesin BJMBR Alves,A.M.B.; Lásaro,M.O.; Almeida,D.F.; Ferreira,L.C.S.. Stimulation of the mammalian immune system by administration of plasmid DNA has been shown to be an important approach for vaccine development against several pathogens. In the present study we investigated the use of DNA vaccines to induce immune responses against an enteric bacterial pathogen, enterotoxigenic Escherichia coli (ETEC). Three plasmid vectors encoding colonization factor antigen I (CFA/I), an ETEC fimbrial adhesin, were constructed. Eukaryotic cells transfected with each of these plasmids expressed the heterologous antigen in different compartments: bound to the cytoplasmic membrane (pRECFA), accumulated in the cytoplasm (pPolyCFA) or secreted to the outside medium (pBLCFA). BALB/c mice were intramuscularly (im) inoculated with purified... Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA vaccines; ETEC; Diarrheal disease; CFA/I fimbriae. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200011 New vaccine strategies against enterotoxigenic Escherichia coli: II: Enhanced systemic and secreted antibody responses against the CFA/I fimbriae by priming with DNA and boosting with a live recombinant Salmonella vaccine BJMBR Lásaro,M.O.; Alves,A.M.B.; Guillobel,H.C.R.; Almeida,D.F.; Ferreira,L.C.S.. The induction of systemic (IgG) and mucosal (IgA) antibody responses against the colonization factor I antigen (CFA/I) of enterotoxigenic Escherichia coli (ETEC) was evaluated in mice primed with an intramuscularly delivered CFA/I-encoding DNA vaccine followed by two oral immunizations with a live recombinant Salmonella typhimurium vaccine strain expressing the ETEC antigen. The booster effect induced by the oral immunization was detected two weeks and one year after the administration of the DNA vaccine. The DNA-primed/Salmonella-boosted vaccination regime showed a synergistic effect on the induced CFA/I-specific systemic and secreted antibody levels which could not be attained by either immunization strategy alone. These results suggest that the combined... Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA vaccine; ETEC; CFA/I fimbriae; Salmonella bivalent vaccine. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200014 Registros recuperados: 3 Primeira ... 1 ... Última

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