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Pascual,Amália N.T.; Suzuki,Karen M.; Almeida,Fernanda S.; Sodré,Leda M.K.; Sofia,Silvia H.. |
In the current literature, information is scarce on which part of the adult insect body is suitable for isolation of genomic DNA for genetic analysis based on DNA-markers. In this study, we evaluated RAPD profiles generated from total genomic DNA isolated from distinct body parts (head, legs, thorax + wings and abdomen) of 12 males of Euglossa pleosticta Dressler. From the total of bands analyzed, 9.0% did not show reproducibility. Percent variations of bands in each body segment were: 1.1% (head); 0.4% (legs); 0.8% (thorax/wings) and 6.7% (abdomen). The much higher variation (chi2one sample = 10.27; df = 1; P < 0.01) in the RAPD profiles obtained by using DNA isolated from abdomen of the euglossine males suggests that this body part of adult insects... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apoidea; Euglossini; Orchid bee; Euglossine bee; Molecular marker. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000600014 |
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Galindo,Bruno A.; Ferreira,Dhiego G.; Apolinário-Silva,Caroline; Terra,Mariana C.; Aprígio,Nícollas G. O.; Ota,Renata R.; Ohara,Willian M.; Almeida,Fernanda S.; Sofia,Silvia H.. |
ABSTRACT Brycon nattereri is an endangered Neotropical fish reported along conserved stretches of the upper Paraná, Tocantins and São Francisco rivers. Populations of this species have been very rare in some Paraná River sub basins. This study analyzes the genetic diversity and population structure of B. nattereri in a restricted area of occurrence recently identified in upper Paraná River basin. Seven microsatellite loci and 497 bp of D-Loop mitochondrial region were examined in 92 individuals from four points along the area of occurrence. Both molecular markers indicated a single population distributed along a stretch of the river approximately 80 km long. Although some of the data suggest an ancient bottleneck, current levels of genetic diversity (H E... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conservation; Endangered species; Molecular markers; Population genetics; Pirapitinga. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000100208 |
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