|
|
| Costa,E.P.; Lopes,F.G.; Pereira,E.C.M.; Queiroz,V.L.D.; Macedo,G.G.; Almeida Neto,J.R.M.; Costa,A.H.A.. |
| A simplified, fast, and innovative method was developed to count the total cell number in blastocysts. Murine blastocysts (N = 195) were used in this study. They were obtained after 10h culture of initial blastocysts, compact morulae grades I and II recovered from superovulated mouse. After culture, the blastococysts were selected to test the new proposal of counting. The process was done after embryo fixation in a sodium citrate solution, and adherence in glass slide. Following, the coloration was done using a fast panoptic coloration kit. As a result, it was possible to identify the blastomeres and count them in each blastocyst. This method provided a fast and effective analysis of the total cell number when compared with other techniques. Moreover, this... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Contagem de células; Embrião; Reprodução. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600032 |
| |
