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Astyanax hastatus Myers, 1928 (Teleostei, Characidae): a new species complex within the genus Astyanax? Genet. Mol. Biol.
Kavalco,Karine Frehner; Brandão,Karina de Oliveira; Pazza,Rubens; Almeida-Toledo,Lurdes Foresti de.
Four populations of Astyanax hastatus Myers 1928 from the Guapimirim River basin (Rio de Janeiro State) were analyzed and three distinct cytotypes identified. These cytotypes presented 2n = 50 chromosomes, with 4M+8SM+10ST+28A (Cytotype A), 8M+10SM+14ST+18A (Cytotype B), 6M+8SM+4ST+32A (Cytotype C) and scanty heterochromatin, mainly located throughout pericentromeric regions of several chromosomal pairs. No homologies with the As-51 satellite DNA were observed in the three cytotypes, although all of them presented multiple 18S rDNA sites, as detected by both silver nitrate staining and FISH (fluorescent in situ hybridization). The application of the term "species complex" in Astyanax is discussed from a cytotaxonomic viewpoint.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Astyanax hastatus; Molecular cytogenetics; Karyotypic evolution; Species complex.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300009
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Cytogenetic characterization of the strongly electric Amazonian eel, Electrophorus electricus (Teleostei, Gymnotiformes), from the Brazilian rivers Amazon and Araguaia Genet. Mol. Biol.
Fonteles,Soraia B.A.; Lopes,Carlos E.; Akama,Alberto; Fernandes,Flora M.C.; Porto-Foresti,Fábio; Senhorini,José A.; Daniel-Silva,Maria de Fátima Z.; Foresti,Fausto; Almeida-Toledo,Lurdes Foresti de.
A karyotype analysis of the electric eel, Electrophorus electricus (Teleostei, Gymnotiformes), a strongly electric fish from northern South America, is presented. Two female specimens were analyzed, one from the Amazon River and one from the Araguaia River. The specimens had a chromosomal number of 2n = 52 (42M-SM + 10A). C-bands were present in a centromeric and pericentromeric position on part of the chromosomes; some interstitial C-bands were also present. Heteromorphic nucleolus organizer regions (NORs) were detected in two chromosome pairs of the specimen from the Amazon River. The chromosome number and karyotype characteristics are similar to those of other Gymnotidae species. The genera Electrophorus and Gymnotus are positioned as the basal lineages...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome; Fish; Gymnotidae; Electric eel.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200010
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Use of chromosome microdissection in fish molecular cytogenetics Genet. Mol. Biol.
Henning,Frederico; Trifonov,Vladimir; Almeida-Toledo,Lurdes Foresti de.
Chromosome microdissection is a technique in which whole chromosomes or chromosomal segments are dissected under an inverted microscope yielding chromosome-specific sequences. Several protocol modifications introduced during the past 15 years reduced the number of chromosomes required for most applications. This is of particular interest to fish molecular cytogenetics, since most species present highly uniform karyotypes which make impossible the collection of multiple copies of the same chromosome. Probes developed in this manner can be used to investigate chromosome homologies in closely related species. Here we describe a protocol recently used in the gymnotiform species group Eigenmannia and review the major steps involved in the generation of these...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fluorescence in situ hybridization; Chromosome painting; Sex chromosomes; Cross-FISH.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200022
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Cytogenetic and molecular analyses in troglobitic and epigean species of Pimelodella (Siluriformes: Heptapteridae) from Brazil Neotropical Ichthyology
Dazzani,Bianca; Garcia,Caroline; Peixoto,Marilena; Trajano,Eleonora; Almeida-Toledo,Lurdes Foresti de.
Samples from seven different locations of the genus Pimelodella were genetically examined, two caves (exclusively subterranean, upper Tocantins River and São Francisco River) and five epigean (from upper Paraná River basin). Cytogenetic analyses revealed the same diploid number (2n=46) for all species besides similarities in both number and location of nucleolar organizer regions and C bands. FISH with 5S rDNA probes and CMA3 staining indicated significant differences among the studied species. Application of PCR-RFLP in ATPase 6 and 8 mitochondrial genes allowed building a minimum evolution phenogram identifying the close evolutionary relationship among groups. Both chromosomal and molecular data were useful to infer the relationships among studied...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cave fish; Cytogenetics; Evolutionary relationships; FISH; PCR-RFLP.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000300016
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