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Factors influencing disease-induced mortality of Pacific oysters Crassostrea gigas 5
Petton, Bruno; Boudry, Pierre; Alunno-bruscia, Marianne; Pernet, Fabrice.
Mortalities of oyster Crassostrea gigas seed associated with ostreid herpesvirus OsHV-1 μVar have been observed in many oyster-producing countries since 2008. The present study, comprised of 4 complementary experiments, aimed to identify factors associated with disease-induced oyster mortality in order to propose mitigation strategies. Our first experiment compared survival of oysters from natural spatfall with others sampled from nurseries, after thermal elevation in the laboratory from <14 to 21°C. A total of 60% of the tested wild seed batches (n = 51) were infected by OsHV-1, exhibited mortality and were able to transmit the disease to cohabited naïve oysters. Comparatively, only 1 out of the 32 tested batches sampled from nurseries presented...
Tipo: Text Palavras-chave: Epidemiology; Risk analyses; Shellfish farming; Virus OsHV-1 mu Var.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00257/36777/35390.pdf
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Understanding the dynamics of δ13C and δ15N in soft tissues of the bivalve Crassostrea gigas facing environmental fluctuations in the context of Dynamic Energy Budgets (DEB) 5
Emmery, Antoine; Lefebvre, Sebastien; Alunno-bruscia, Marianne; Kooijman, S. A. L. M..
We studied the dynamics of stable isotopes δ13C and δ15N of an opportunistic suspension feeder the Pacific oyster (Crassostrea gigas) to better understand the factors that influence the trophic enrichment (trophic-shift, Δ) between primary producers and consumers. Most of the previous studies on this topic do not quantify mass fluxes or isotopic discrimination phenomena in the organism, which are two pillars in isotope ecology. We used a dynamic energy budget (DEB) approach (Kooijman, 2010) to quantify i) the fluxes of elements and isotopes in C. gigas soft tissues and ii) the impact of the scaled feeding level, the organism mass and the isotopic ratio of food on the "trophic-shift" Δ, and isotope turnover in tissues. Calibration and parametrization...
Tipo: Text Palavras-chave: Oyster; Isotopic ratio; Discrimination; Trophic-shift; Diet; DEB theory.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00043/15434/12784.pdf
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Global change and climate-driven invasion of the Pacific oyster (Crassostrea gigas) along European coasts: a bioenergetics modelling approach 5
Thomas, Yoann; Pouvreau, Stephane; Alunno-bruscia, Marianne; Barille, Laurent; Gohin, Francis; Bryere, Philippe; Gernez, Pierre.
Aim The spread of non-indigenous species in marine ecosystems world-wide is one of today's most serious environmental concerns. Using mechanistic modelling, we investigated how global change relates to the invasion of European coasts by a non-native marine invertebrate, the Pacific oyster Crassostrea gigas. Location Bourgneuf Bay on the French Atlantic coast was considered as the northern boundary of C. gigas expansion at the time of its introduction to Europe in the 1970s. From this latitudinal reference, variations in the spatial distribution of the C. gigas reproductive niche were analysed along the north-western European coast from Gibraltar to Norway. Methods The effects of environmental variations on C. gigas physiology and phenology were studied...
Tipo: Text Palavras-chave: Biogeographical range expansion; Crassostrea gigas; DEB model; European coasts; Functional traits; Global change; Individual-based model; Invasive species; Remote-sensing.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00287/39828/38459.pdf
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Modelling growth and reproduction in the Pacific oyster Crassostrea gigas : 1. What's new with the oyster-DEB model? 5
Bourles, Yves; Pouvreau, Stephane; Alunno-bruscia, Marianne; Leguay, Didier; Arnaud, Christophe; Goulletquer, Philippe; Kooijman, S. A. L. M..
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Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3330.pdf
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Sources of paralytic shellfish toxin accumulation variability in the Pacific oyster Crassostrea gigas 5
Pousse, Emilien; Flye-sainte-marie, Jonathan; Alunno-bruscia, Marianne; Hegaret, Helene; Jean, Fred.
This study was designed to assess the contribution of feeding behavior to inter-individual variability of paralytic shellfish toxin (PST) accumulation in the Pacific oyster Crassostrea gigas. For this purpose 42 oysters were exposed for 2 days to non-toxic algae and then for 2 other days to the PST producer Alexandrium minutum. Individual clearance rate (CR) of oysters was continuously monitored over the 4 days using an ecophysiological measurement system. Comparison of CR values when exposed to toxic and non toxic algae allowed to estimate a clearance rate inhibition index (CRII). Toxin concentration of oysters was quantified at the end of the experiment. These data allowed to estimate the toxin accumulation efficiency (TAE) as the ratio of toxin...
Tipo: Text Palavras-chave: Alexandrium minutum; Paralytic shellfish poisoning; Accumulation; Clearance rate; Feeding behavior; Pacific oyster.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00416/52789/53688.pdf
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A single bio-energetics growth and reproduction model for the oyster Crassostrea gigas in six Atlantic ecosystems 5
Alunno-bruscia, Marianne; Bourles, Yves; Maurer, Daniele; Robert, Stephane; Mazurie, Joseph; Gangnery, Aline; Goulletquer, Philippe; Pouvreau, Stephane.
Many studies based on bioenergetics growth models have investigated the effects of environmental factors on oyster (Crassostrea gigas) growth and physiology. However, most of these models are site-specific and cannot be applied to other culture sites without the re-estimation of parameters or re-formulation of some processes. We aimed to develop a generic growth model suitable for application in contrasting environments, with a constant set of parameters. We tested the oyster-DEB model (Bourlès et al. 2009) for the stimulation of C. gigas growth in different cohorts (spats and adults) at major shellfish culture sites in France, in several years: Arcachon (1993–1994); Marennes-Oléron (2007); Quiberon (1999, 2000, 2001); Rade de Brest (2008); Baie du...
Tipo: Text Palavras-chave: DEB theory; Modelling; Bivalves; Crassostrea gigas; Phytoplankton; Temperature effect; Coastal environment..
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00043/15403/12762.pdf
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The dinoflagellate Alexandrium minutum affects development of the oyster Crassostrea gigas, through parental or direct exposure 5
Castrec, Justine; Hegaret, Helene; Alunno-bruscia, Marianne; Picard, Mailys; Soudant, Philippe; Petton, Bruno; Boulais, Myrina; Suquet, Marc; Queau, Isabelle; Ratiskol, Dominique; Foulon, Valentin; Le Goic, Nelly; Fabioux, Caroline.
Harmful algal blooms are a threat to aquatic organisms and coastal ecosystems. Among harmful species, the widespread distributed genus Alexandrium is of global importance. This genus is well-known for the synthesis of paralytic shellfish toxins which are toxic for humans through the consumption of contaminated shellfish. While the effects of Alexandrium species upon the physiology of bivalves are now well documented, consequences on reproduction remain poorly studied. In France, Alexandrium minutum blooms have been recurrent for the last decades, generally appearing during the reproduction season of most bivalves including the oyster Crassostrea gigas. These blooms could not only affect gametogenesis but also spawning, larval development or juvenile...
Tipo: Text Palavras-chave: Harmful algal bloom (HAB); Paralytic shellfish toxin (PST); Crassostrea gigas; Gametes; Larvae.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00469/58099/60512.pdf
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Vibrio crassostreae, a benign oyster colonizer turned into a pathogen after plasmid acquisition 5
Bruto, Maxime; James, Adele; Petton, Bruno; Labreuche, Yannick; Chenivesse, Sabine; Alunno-bruscia, Marianne; Polz, Martin F.; Le Roux, Frederique.
Vibrios are frequently associated with oyster mortality; however whether they are the primary causative agent or secondary opportunistic colonizers is not well understood. Here we combine analysis of natural infection dynamics, population genomics and molecular genetics to ask (i) to what extent oysters are passively colonized by Vibrio population present in the surrounding water, (ii) how populations turn over during pathogenicity events and (iii) what genetic factors are responsible for pathogenicity. We identified several populations of Vibrio preferentially associated with oyster tissues. Among these, Vibrio crassostreae is particularly abundant in diseased animals while nearly absent in the surrounding water, and its pathogenicity is correlated with...
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Ano: 2017 URL: http://archimer.ifremer.fr/doc/00360/47137/49044.pdf
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Applying Dynamic Energy Budget (DEB) theory to simulate growth and bio-energetics of blue mussels under low seston conditions 5
Rosland, R; Strand, O; Alunno-bruscia, Marianne; Bacher, Cedric; Strohmeier, T.
A Dynamic Energy Budget (DEB) model for simulation of growth and bioenergetics of blue mussels (Mytilus edulis) has been tested in three low seston sites in southern Norway. The observations comprise four datasets from laboratory experiments (physiological and biometrical mussel data) and three datasets from in situ growth experiments (biometrical mussel data). Additional in situ data from commercial farms in southern Norway were used for estimation of biometricalrelationships in the mussels. Three DEB parameters (shape coefficient, half saturation coefficient, and somatic maintenance rate coefficient) were estimated from experimental data, and the estimated parameters were complemented with parameter values from literature to establish a basic parameter...
Tipo: Text Palavras-chave: Low seston; Modelling; DEB theory; Mytilus edulis; Mussel culture.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6266.pdf
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Qualification zoo-sanitaire du naissain de captage 2014 de l'huître creuse Crassostrea gigas (QUALIF) - Détection précoce des maladies en épreuve thermique de laboratoire 5
Petton, Bruno; Le Grand, Jacqueline; Riobe, Marion; Mingant, Christian; Ratiskol, Dominique; Petton, Sebastien; Alunno-bruscia, Marianne.
Depuis 2008, des mortalités massives d’huîtres creuses âgées de moins d’un an sont relevées sur le littoral français dès que la température de l’eau de mer atteint le seuil de 16°C. Ces mortalités de naissains sont associées à la détection du virus OsHV-1 μVar. Depuis 2010, des travaux de qualification zoo-sanitaire ont montré qu’environ un lot de naissains de captage sur deux était infecté par le virus avant l’apparition des mortalités dans le milieu naturel. En 2014, dans le cadre de la première action QUALIF, les résultats de la qualification zoo-sanitaire des naissains du captage de l’année 2013 à l’échelle nationale ont mis en évidence le caractère infectieux des maladies développées par ces naissains porteurs latents d’OsHV-1 μVar. L’objectif 2015 du...
Tipo: Text Palavras-chave: Huitre creuse; Naissain; Maladie; OsHV-1 μVar; Epreuve thermique.; Pacific oyster; Seed; Disease; OsHV-1 μVar; Thermal challenge..
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00335/44587/44305.pdf
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Immune-suppression by OsHV-1 viral infection causes fatal bacteraemia in Pacific oysters 5
De Lorgeril, Julien; Lucasson, Aude; Petton, Bruno; Toulza, Eve; Montagnani, Caroline; Clerissi, Camille; Vidal-dupiol, Jeremie; Chaparro, Cristian; Galinier, Richard; Escoubas, Jean Michel; Haffner, Philippe; Degremont, Lionel; Charriere, Guillaume; Lafont, Maxime; Delort, Abigail; Vergnes, Agnes; Chiarello, Marlene; Faury, Nicole; Rubio, Tristan; Leroy, Marc; Perignon, Adeline; Regler, Denis; Morga, Benjamin; Alunno-bruscia, Marianne; Boudry, Pierre; Le Roux, Frederique; Destoumieux-garzon, Delphine; Gueguen, Yannick; Mitta, Guillaume.
Infectious diseases are mostly explored using reductionist approaches despite repeated evidence showing them to be strongly in fluenced by numerous interacting host and environmental factors. Many diseases with a complex aetiology therefore remain misunderstood. By developing a holistic approach to tackle the complexity of interactions, we decipher the complex intra-host interactions underlying Pacific oyster mortality syndrome affecting juveniles of Crassostrea gigas, the main oyster species exploited worldwide. Using experimental infections reproducing the natural route of infection and combining thorough molecular analyses of oyster families with contrasted susceptibilities, we demonstrate that the disease is caused by multiple infection with an initial...
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00461/57234/59228.pdf
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Modelling isotopes dynamics in soft tissues of Crassostrea gigas in the context of DEB theory to study the trophic ecology of oysters at large spatial scale 5
Emmery, Antoine; Lefebvre, Sebastien; Alunno-bruscia, Marianne; Kooijman, S. A. L. M..
Biological performances (growth and reproduction) of intertidal bivalves mainly rely on environmental factors such as water temperature and food sources. Both quality and quantity of bivalve food sources, however, are not easy to determine because of complex features of coastal ecosystem functioning, such as high spatial heterogeneity. This is particularly critical for oyster (Crassostrea gigas) culture in France when quantifying and explaining the variability in growth performances of oysters among culture sites at a large spatial scale. In this context, insights for new operational tools to characterise trophic features of coastal ecosystems are needed. The aim of the present study is to couple natural isotope tracers and DEB modeling to describe and...
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Ano: 2009 URL: http://archimer.ifremer.fr/doc/00032/14370/11659.pdf
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Experimental and natural cathodoluminescence in the shell of Crassostrea gigas from Thau lagoon (France): ecological and environmental implications 5
Langlet, D; Alunno-bruscia, Marianne; Rafelis, M; Renard, M; Roux, M; Schein, E; Buestel, Dominique.
We present a cathodoluminescence (CL) study of growth layer deposition in the shell of the oyster Crassostrea gigas. CL is based on the physical properties of lattice-bound manganese (Mn2+), which is the main activator in calcium carbonate. Our study involved chemical marking by immersing individuals in seawater to which manganese chloride had been added, and subsequent reading of the shell with CL microscopy coupled with numeric treatment of microphotographs; CL emission was analyzed using a scanning electron microscope coupled to a spectrometer. Since the marking did not harm the oysters, repeated markings were possible, allowing validation of the inferences made from analysis of the shell rings. Oysters reared in a French Mediterranean lagoon (Thau...
Tipo: Text Palavras-chave: Mediterranean lagoon; Manganese marking; Carbonate biomineraliation; Shell growth; Cathodoluminescence; Oyster.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1979.pdf
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Molecular Targets for Coevolutionary Interactions Between Pacific Oyster Larvae and Their Sympatric Vibrios 5
Wegner, K. Mathias; Piel, Damien; Bruto, Maxime; John, Uwe; Mao, Zhijuan; Alunno-bruscia, Marianne; Petton, Bruno; Le Roux, Frederique.
Bacteria of the Vibrio genus are the most predominant infectious agents threatening marine wildlife and aquaculture. Due to the large genetic diversity of these pathogens, the molecular determinants of Vibrio virulence are only poorly understood. Furthermore, studies tend to ignore co-evolutionary interactions between different host populations and their locally encountered Vibrio communities. Here, we explore the molecular targets of such co-evolutionary interactions by analyzing the genomes of nine Vibrio strains from the Splendidus-clade showing opposite virulence patterns towards two populations of Pacific oysters introduced into European Wadden Sea. By contrasting Vibrio phylogeny to their host specific virulence patterns, we could identify two core...
Tipo: Text Palavras-chave: Virulence; Host-pathogen interaction; Local adaptation; Biological invasion; Emerging disease; Wadden Sea.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00512/62409/66669.pdf
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Modelling growth and reproduction of the Pacific oyster Crassostrea gigas: Advances in the oyster-DEB model through application to a coastal pond 5
Bourles, Yves; Alunno-bruscia, Marianne; Pouvreau, Stephane; Tollu, Guillaume; Leguay, Didier; Arnaud, Christophe; Goulletquer, Philippe; Kooijman, S.
A bio-energetic model, based on the DEB theory exists for the Pacific oyster Crassostrea gigas. Pouvreau et al. [Pouvreau, S., Bourles, Y., Lefebvre, S., Gangnery, A., Alunno-Bruscia, M., 2006. Application of a dynamic energy budget model to the Pacific oyster, C. gigas, reared under various environmental conditions. J. Sea Res. 56, 156–167.] successfully applied this model to oysters reared in three environments with no tide and low turbidity, using chlorophyll a concentration as food quantifier. However, the robustness of the oyster-DEB model needs to be validated in varying environments where different food quantifiers reflect the food available for oysters, as is the case in estuaries and most coastal ecosystems. We therefore tested the oyster-DEB...
Tipo: Text Palavras-chave: Coastal Environment; Temperature effect; Food Quantifiers; Crassostrea gigas; Bivalves; Modelling; DEB theory.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6436.pdf
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Observer, Analyser et Gérer la variabilité de la reproduction et du recrutement de l’huître creuse en France : Le Réseau Velyger. Rapport annuel 2012 5
Pouvreau, Stephane; Bellec, Gwenael; Le Souchu, Pierrick; Queau, Isabelle; Talarmain, Eric; Alunno-bruscia, Marianne; Auby, Isabelle; Maurer, Daniele; Barbier, Claire; Tournaire, Marie-pierre; D'Amico, Florence; Rumebe, Myriam; Fleury, Elodie; Langlade, Aime; Bouget, Jean-francois; Collin, Karine; Fortune, Mireille; Hitier, Benoist; Bedier, Edouard; Robert, Stephane; Guilpain, Patrice; Grizon, James; Seugnet, Jean-luc; Guesdon, Stephane; Lagarde, Franck; Le Gall, Patrik; Messiaen, Gregory; Roque D'Orbcastel, Emmanuelle; Gohin, Francis; Bryere, Philippe; Quemener, Loic; Repecaud, Michel; Bucas, Karenn; Barbot, Stephane; Guillemot, Anne; Nonnotte, Lidwine; Duros, Wenceslas; Bonnat, Armel; Tarot, Stephane; Mille, Dominique; Geay, Amelie; Bouquet, Anne-lise.
La conchyliculture, et principalement l’élevage de l’huître creuse, Crassostrea gigas, constitue la principale activité aquacole française. Cette activité repose, en grande partie, sur le recrutement naturel de l’espèce qui assure 70% des besoins en jeunes huîtres (naissain) : cette activité de collecte s’appelle le captage. Les deux principaux centres de captage en France sont les bassins d’Arcachon et de Marennes-Oléron. Or, depuis une dizaine d'années, sur le Bassin d'Arcachon, le captage devient très variable: à des années de captage nul (par exemple les années 2002, 2005, 2007) ou faible (2009, 2010, 2011) succèdent des années excellentes voire pléthoriques (les années 2003, 2006, 2008, 2012). A Marennes-Oléron, cette variabilité existe, mais s’avère...
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Ano: 2013 URL: http://archimer.ifremer.fr/doc/00135/24648/22681.pdf
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Modelling paralytic shellfish toxins (PST) accumulation in Crassostrea gigas by using Dynamic Energy Budgets (DEB) 5
Pousse, Emilien; Flye-sainte-marie, Jonathan; Alunno-bruscia, Marianne; Hegaret, Helene; Rannou, Eric; Pecquerie, Laure; Marques, Goncalo M.; Thomas, Yoann; Castrec, Justine; Fabioux, Caroline; Long, Marc; Lassudrie, Malwenn; Hermabessiere, Ludovic; Amzil, Zouher; Soudant, Philippe; Jean, Fred.
As other filter-feeders, Crassostrea gigas can concentrate paralytic shellfish toxins (PST) by consuming dinoflagellate phytoplankton species like Alexandrium minutum. Intake of PST in oyster tissues mainly results from feeding processes, i.e. clearance rate, pre-ingestive sorting and ingestion that are directly influenced by environmental conditions (trophic sources, temperature). This study aimed to develop a mechanistic model coupling the kinetics of PST accumulation and bioenergetics in C. gigas based on Dynamic Energy Budget (DEB) theory. For the first time, the Synthesizing Units (SU) concept was applied to formalize the feeding preference of oysters between non-toxic and toxic microalgae. Toxin intake and accumulation were both dependent on the...
Tipo: Text Palavras-chave: Alexandrium minutum; Paralytic shellfish toxins (PST); Dynamic Energy Budget (DEB); Modelling; Pacific oyster.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00455/56662/58407.pdf
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Modelling spatio-temporal variability of Mytilus edulis (L.) growth by forcing a dynamic energy budget model with satellite-derived environmental data 5
Thomas, Yoann; Mazurie, Joseph; Alunno-bruscia, Marianne; Bacher, Cedric; Bouget, Jean-francois; Gohin, Francis; Pouvreau, Stephane; Struski, Caroline.
In order to assess the potential of various marine ecosystems for shellfish aquaculture and to evaluate their carrying capacities, there is a need to clarify the response of exploited species to environmental variations using robust ecophysiological models and available environmental data. For a large range of applications and comparison purposes, a non-specific approach based on ‘generic’ individual growth models offers many advantages. In this context, we simulated the response of blue mussel (Mytilus edulis L.) to the spatio-temporal fluctuations of the environment in Mont Saint-Michel Bay (North Brittany) by forcing a generic growth model based on Dynamic Energy Budgets with satellite-derived environmental data (i.e. temperature and food). After a...
Tipo: Text Palavras-chave: Mussel; DEB; SeaWifs; Aquaculture; Site Selection; Resource Management.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00037/14836/14537.pdf
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Individual mussel growth model using DEB (Dynamic Energy Budget) theory: revisiting the DEB parameter values for Mytilus edulis 5
Flye Sainte Marie, Jonathan; Alunno-bruscia, Marianne; Gangnery, Aline; Rannou, Eric; Rosland, Rune; Strand, Øivind.
Mussel aquaculture is well developed in various ecosystems of temperate waters. In the aim of developing predictive tools for management of mussel aquaculture, a better understanding of relationships between the environmental conditions and mussel growth is necessary. For this purpose, development of bioenergetic models, linking environmental variables (especially food resource and temperature) and mussel growth and reproduction, are of a particular interest. Dynamic Energy Budget (DEB) theory offers a general framework to study energy flows in organisms, from assimilation to use for maintenance, growth and reproduction. This theory is appropriate for growth modelling of marine organisms and have first been applied in 1993 to model mussel growth....
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Ano: 2009 URL: http://archimer.ifremer.fr/doc/00032/14368/11658.pdf
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Body size-density relationship for Mytilus edulis in an experimental food-regulated situation 5
Alunno-bruscia, Marianne; Petraitis, P; Bourget, E; Frechette, Marcel.
We grew mussels (Mytilus edulis) under two different food regimes and eight population density levels to estimate the joint effects of density and biomass on their growth and survival and to determine the shape of the biomass-density (B-IV) relationship. Mussels were reared for 22 months, between December 1994 and October 1996, in 1-L experimental chambers supplied with natural seston. Growth in shell length, individual wet mass and ash free dry mass (m) decreased with decreasing food availability and increasing population density. Survival was negatively correlated with density but did not differ significantly between food regimes during the first year. Variations in concentration of available food did not alter the effects of crowding on mussels, as...
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Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-6791.pdf
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