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	Provedor de dados BJMBR (2) Arq. Bras. Med. Vet. Zootec. (1) Autor Alvares,L.E. (3) Coutinho,L.L. (3) Gabriel,J.E. (2) Alves,H.J. (1) Corrente,J.E. (1) Mantoani,A. (1) Packer,I.U. (1) Paz,C.C.P. (1) Mais... Palavra-chave BrdU (1) Cellular proliferation (1) Chicken development (1) Chicken embryo (1) Competitive RT-PCR (1) Gene expression (1) In situ hybridization (1) MRNA abundance (1) MyoD (1) Myogenesis (1) Myogenic regulatory factors (1) Myostatin (1) Quantitative RT-PCR (1) Standard-curve (1) Temporal expression (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Info:eu-repo/semantics/other (1) Ano 2006 (1) 2003 (2) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Temporal expression pattern of myostatin transcripts during chicken embryogenesis Arq. Bras. Med. Vet. Zootec. Gabriel,J.E.; Alvares,L.E.; Paz,C.C.P.; Packer,I.U.; Coutinho,L.L.. No presente estudo, estimou-se a abundância dos transcritos da miostatina foi estimada durante a embriogênese de galinha por análises de RT-PCR competitiva. Níveis basais de mRNA desse gene foram detectados até o estádio HH15, enquanto acúmulos significativos nesses níveis foram observados apenas no estádio HH24, seguido por redução na abundância desses transcritos a partir do estádio HH26. Tais descobertas preliminares proporcionam informações relevantes sobre a ativação do fator de crescimento miostatina durante o desenvolvimento in ovo de aves. Tipo: Info:eu-repo/semantics/other Palavras-chave: Chicken embryo; Myostatin; Temporal expression; MRNA abundance; Competitive RT-PCR. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000500033 Influence of the neural tube/notochord complex on MyoD expression and cellular proliferation in chicken embryos BJMBR Alves,H.J.; Alvares,L.E.; Gabriel,J.E.; Coutinho,L.L.. Important advances have been made in understanding the genetic processes that control skeletal muscle formation. Studies conducted on quails detected a delay in the myogenic program of animals selected for high growth rates. These studies have led to the hypothesis that a delay in myogenesis would allow somitic cells to proliferate longer and consequently increase the number of embryonic myoblasts. To test this hypothesis, recently segmented somites and part of the unsegmented paraxial mesoderm were separated from the neural tube/notochord complex in HH12 chicken embryos. In situ hybridization and competitive RT-PCR revealed that MyoD transcripts, which are responsible for myoblast determination, were absent in somites separated from neural tube/notochord... Tipo: Info:eu-repo/semantics/article Palavras-chave: Chicken development; MyoD; Myogenesis; In situ hybridization; BrdU; Cellular proliferation. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200005 Standard-curve competitive RT-PCR quantification of myogenic regulatory factors in chicken embryos BJMBR Alvares,L.E.; Mantoani,A.; Corrente,J.E.; Coutinho,L.L.. The reverse transcription-polymerase chain reaction (RT-PCR) is the most sensitive method used to evaluate gene expression. Although many advances have been made since quantitative RT-PCR was first described, few reports deal with the mathematical bases of this technique. The aim of the present study was to develop and standardize a competitive PCR method using standard-curves to quantify transcripts of the myogenic regulatory factors MyoD, Myf-5, Myogenin and MRF4 in chicken embryos. Competitor cDNA molecules were constructed for each gene under study using deletion primers, which were designed to maintain the anchorage sites for the primers used to amplify target cDNAs. Standard-curves were prepared by co-amplification of different amounts of target cDNA... Tipo: Info:eu-repo/semantics/article Palavras-chave: Quantitative RT-PCR; Standard-curve; Myogenic regulatory factors; Gene expression. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200004 Registros recuperados: 3 Primeira ... 1 ... Última

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