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	Provedor de dados BJID (1) Rev. Bras. Frutic. (1) Autor Alves,Paulo César Martins (2) Amaral,Paulo de Paiva Rosa (1) Arrym,Mauro Pedromonico (1) Capdeville,Guy de (1) Castelhano,Mariana Virginello (1) Guimarães,Fernando (1) Lemos,Renata Muller Banzato Pinto de (1) Levy,Carlos Emilio (1) Martins,Natália Florêncio (1) Mazzola,Taís Nitsch (1) Silva,Felipe Rodrigues da (1) Silva,Marcos Tadeu Nolasco da (1) Souza Júnior,Manoel Teixeira (1) Zaccariotto,Tânia Regina (1) Mais... Palavra-chave BCG (1) C. papaya (1) Caricaceae (1) Citotoxicity (1) HIV/Aids (1) Immunologic (1) Nucleotide-binding site (NBS) (1) Papaya (1) Tuberculosis (1) V. cauliflora (1) Youth (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2019 (1) 2006 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Preservation of cytotoxic granule production in response to mycobacterial antigens by T-lymphocytes from vertically HIV-infected Brazilian youth on effective combined antiretroviral therapy BJID Arrym,Mauro Pedromonico; Alves,Paulo César Martins; Castelhano,Mariana Virginello; Mazzola,Taís Nitsch; Lemos,Renata Muller Banzato Pinto de; Zaccariotto,Tânia Regina; Levy,Carlos Emilio; Guimarães,Fernando; Silva,Marcos Tadeu Nolasco da. ABSTRACT Background: HIV infection harms adaptive cellular immunity mechanisms. Long-term virological control by combined antiretroviral therapy (cART) reduces the risk of mycobacterial infections. Thus, we aimed to study cellular responses to mycobacterial antigens in 20 HIV-infected adolescents with at least one year of virological control (HIV-RNA <40 copies/mL) and 20 healthy adolescents. Methods: We evaluated CD8 and γδ T-cell degranulation by measurement of CD107a membrane expression after stimulation with lysates from BCG (10 µg/mL) and H37RA Mycobacterium tuberculosis (Mtb, 10 µg/mL). Immune activation and antigen-presenting ability were also assessed by determination of HLA-DR, CD80, and CD86 markers. Results: TCR γδ T-cell CD107a expression... Tipo: Info:eu-repo/semantics/article Palavras-chave: HIV/Aids; BCG; Youth; Tuberculosis; Citotoxicity; Immunologic. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000300151 Identification and characterization of a resistance gene analog (RGA) from the Caricaceae Dumort family Rev. Bras. Frutic. Amaral,Paulo de Paiva Rosa; Alves,Paulo César Martins; Martins,Natália Florêncio; Silva,Felipe Rodrigues da; Capdeville,Guy de; Souza Júnior,Manoel Teixeira. The majority of cloned resistance (R) genes characterized so far contain a nucleotide-binding site (NBS) and a leucine-rich repeat (LRR) domain, where highly conserved motifs are found. Resistance genes analogs (RGAs) are genetic markers obtained by a PCR-based strategy using degenerated oligonucleotide primers drawn from these highly conserved "motifs". This strategy has the advantage of the high degree of structural and amino acid sequence conservation that is observed in R genes. The objective of the present study was to search for RGAs in Carica papaya L. and Vasconcellea cauliflora Jacq. A. DC. Out of three combinations of primers tested, only one resulted in amplification. The amplified product was cloned in pCR2.1TOPO and than sequenced using M13... Tipo: Info:eu-repo/semantics/article Palavras-chave: Caricaceae; Nucleotide-binding site (NBS); V. cauliflora; C. papaya; Papaya. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452006000300026 Registros recuperados: 2 Primeira ... 1 ... Última

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