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| Vitiello,Glauco Akelinghton Freire; Guembarovski,Roberta Losi; Oliveira,Carlos Eduardo Coral de; Amarante,Marla Karine; Perim,Aparecida de Lourdes; Watanabe,Maria Angelica Ehara. |
| Hematological malignancies (HM) are a group of neoplastic diseases that arise from hematologic cell lineages. Transforming growth factor beta 1 (TGFβ1) is shown to negatively regulate normal and malignant hematopoiesis and, in immunological context, to promote T cell exhaustion and generation of regulatory T cells, which are shown to be deleterious in cancer, by the induction of transcription factor FOXP3 expression. The present study aimed to evaluate TGFB1 exon-1 rs1800470 and FOXP3 intron-1 rs2232365 polymorphisms in relation to HM susceptibility. DNA was extracted from blood samples of 43 HM patients and 142 neoplasia-free individuals and polymorphisms were analyzed by allelic-specific PCR. Association analysis was assessed by the Odds Ratio (OR) with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hematological malignancies; TGFB1; FOXP3; Genetic polymorphism; Susceptibility. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400553 |
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| Watanabe,Maria Angelica Ehara; Miquelão,Ana Karina Melim Benthien; Oliveira,Carlos Eduardo Coral de; Oliveira,Karen Brajão de; Nasser,Thiago Franco; Aoki,Mateus Nóbrega; Suzuki,Patrícia Sayuri; Amarante,Marla Karine; Venâncio,Emerson José; Azevedo,Elbens Marcos Minoreli de. |
| The purpose of the present study was to evaluate the sexual transmission of GBV-C/HGV, through RNA detection in cervicovaginal smears. Therefore the GBV-C/HGV RNA in cervicovaginal smears from apparently healthy women was investigated using routine proceedings for prophylactic screening to cervical cancer. GBV-C/HGV RNA was detected by reverse transcriptase and polymerase chain reaction (RT-PCR). Only one woman presented co-infection with human papilloma virus (HPV). The GBV-C/HGV RNA was detected in 13/73 (17.57%) healthy women and it's prevalence in participating women between 28-43 years old was 53.85%. No association was found with GBV-C/HGV for the age of first sexual intercourse and number of pregnancies. In GBV-C/HGV RNA positive women, 69.23% were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: GBV-C/HGV RNA; Healthy women; Cervicovaginal smears. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500007 |
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| Custodio,Luiz Antonio; Saito,Alexandre; Amarante,Marla Karine; Fujita,Thiago Cezar; Perim,Aparecida de Lourdes; Costa,Ivete Conchon; Felipe,Ionice; Jankevicius,Shiduca Itow. |
| In the present study, nasal mucus from patients with leprosy were analyzed by PCR using specific primers for Lsr2 gene of Mycobacterium leprae. The presence of Lsr2 gene in the nasal mucus was detected in 25.80% of patients with paucibacillari leprosy, and 23.07% of contacts. Despite the absence of clinical features in the contact individuals, it was possible to detect the presence of Lsr2 gene in the nasal mucus of these individuals. Therefore, PCR detection of M. leprae targeting Lsr2 gene using nasal mucus samples could contribute to early diagnosis of leprosy. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium leprae; Leprosy; Hanseniase; Nasal mucus; PCR. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300007 |
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| Amarante,Marla Karine; Ariza,Carolina Batista; Oliveira,Carlos Eduardo Coral de; Gualtieri,Karina de Almeida; Oda,Julie Massayo Maeda; Watanabe,Maria Angelica Ehara. |
| ABSTRACT Inulin is an effective prebiotic and its potential in modulating systemic immunity have been proposed. A subpopulation of T cells, named T regulatory cells (Tregs), expressing the Forkhead boxP3 transcription factor are key mediators of peripheral tolerance and suppress undesirable immune responses. These Tregs can be induced by cytokine transforming growth factor beta (TGF-β) and interleukin 10 (IL-10). This work aimed to evaluate inulin effects on human peripheral blood mononuclear cells (PBMC) in vitro. PBMC were incubated with inulin, and the expression of TGF-(1, FOXP3 and IL-10 was analyzed. Increased supernatant IL-10 levels were observed in PBMC of inulin-treated group (p=0.03). Moreover, FOXP3 gene expression was 7.6 fold higher in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Inulin; FOXP3; TGF-β1; IL-10; PBMC. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100417 |
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