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Setting of a procedure for experimental incubation of Pacific oyster (Crassostrea gigas) embryos ArchiMer
Suquet, Marc; Amourda, Christopher; Mingant, Christian; Queau, Isabelle; Lebrun, Luc; Brizard, Raphael.
Standardized experimental protocols designed to study Pacific oyster (Crassostrea gigas) gamete quality have not been previously published. Gamete sampling variations and confounding effect of interacting factors result in large variations between replicates, decreasing the effects of the studied factors. This work aims at defining a standardized procedure for incubation of oyster embryos designed for experimental purposes. In a first phase, four experiments were developed to improve embryo sampling and handling. They showed that a minimum of 50 embryos must be counted to decrease the variation between counting replicates. For reliable results, sampling must be carried out from 0 to 7 min after a careful agitation of seawater containing embryos...
Tipo: Text Palavras-chave: Crassostrea gigas; Pacific oyster; Experimental; Embryo; Incubation.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3593.pdf
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