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Improved growth model for two-stage continuous cultures of Lactobacillus helveticus Electron. J. Biotechnol.
Bouguettoucha,Abdallah; Balannec,Béatrice; Nacef,Saci; Amrane,Abdeltif.
An unstructured model for growth and lactic acid production during two-stage continuous cultures of Lactobacillus helveticus was previously developed. The Verlhust model was considered to describe growth kinetics. Production models was based on modified Luedeking-Piret expressions involving an inhibitory effect for the first stage (seed culture) and a nutritional limitation effect for the second stage (culture). To account for the decrease of the biomass concentration observed in the second stage, the dilution rate Dc was replaced by an exponential term of the dilution rate <img border=0 width=84 height=36 src="http://fbpe/img/ejb/v13n5/a07/image002.gif" align=middle>in the growth and product relations. Contrarily to the previous model, the important...
Tipo: Journal article Palavras-chave: Continuous culture; Growth; Lactic acid fermentation; Substrate limitation; Unstructured models.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500007
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Kinetic analysis and effect of culture medium and coating materials during free and immobilized cell cultures of Bifidobacterium animalis subsp. lactis Bb 12 Electron. J. Biotechnol.
Jalili,Hasan; Razavi,Hadi; Safari,Mohammad; Amrane,Abdeltif.
Microencapsulation technique appears helpful for more protection of Bifidobacteria against acid inhibitory effect. The effect of medium composition and product inhibitory in free cell culture, as well as the effect of the coating materials in immobilized cells, on biomass growth, acid production and substrate utilization kinetics of Bifidobacterium animalis subsp. lactis Bb 12 in uncontrolled batch fermentation was examined. The Monod and the Luedeking and Piret equations with a product inhibition term involving toxic power terms improved model efficiency for both growth and production. The model showed that media and coating materials had an effect on toxic power terms. Cell immobilization had a positive impact on B. animalis culture. Kinetic analysis...
Tipo: Journal article Palavras-chave: Bifidobacterium; Immobilization; Kinetic models; Medium composition.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300002
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Sequential use of ammonium and leucine as nitrogen sources during growth of Geotrichum candidum on a glucose based medium Electron. J. Biotechnol.
Adour,Lydia; Bude,Florence; Amrane,Abdeltif.
Geotrichum candidum growth on ammonium and leucine as nitrogen sources and glucose as a carbon source was examined. A clear preference of G. candidum for ammonium over leucine as a nitrogen source was shown. Indeed, ammonium was completely exhausted at the end of exponential growth after less than 35 hrs of culture; in contrast only 5% of leucine was concomitantly assimilated. Growth continued at slower rates on glucose and leucine as carbon and nitrogen sources respectively, and at the end of culture (185 hrs), leucine was completely exhausted.
Tipo: Journal article Palavras-chave: Amino acids; Batch culture; Diauxic growth; Geotrichum candidum; Growth kinetics.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100013
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