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	Provedor de dados BJMBR (1) Autor Andrade,A.S.R. (1) Tunes,H. (1) Vilela,L. (1) Palavra-chave Gel filtration (1) Glucagon (1) Insulin (1) Ion-exchange chromatography (1) Protein purification (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 1997 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Purification of bovine pancreatic glucagon as a by-product of insulin production BJMBR Andrade,A.S.R.; Vilela,L.; Tunes,H.. A process for purifying bovine pancreatic glucagon as a by-product of insulin production is described. The glucagon-containing supernatant from the alkaline crystallization of insulin was precipitated using ammonium sulfate and isoelectric precipitation. The isoelectric precipitate containing glucagon was then purified by ion-exchange chromatography on Q-Sepharose FF, gel filtration on Sephadex G-25 and ion-exchange chromatography on S-Sepharose FF. A pilot scale test was performed with a recovery of 87.6% and a purification factor of 8.78 for the first chromatographic step, a recovery of 75.1% and a purification factor of 3.90 for the second, and a recovery of 76.2% and a purification factor of 2.36 for the last one. The overall yield was 50%, a... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucagon; Protein purification; Gel filtration; Ion-exchange chromatography; Insulin. Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200006 Registros recuperados: 1 Primeira ... 1 ... Última

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