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| Paula,Daniella A. J.; Andrade,Estefânia S.; Murgas,Luis D. S.; Felizardo,Viviane O.; Winkaler,Elissandra U.; Zeviani,Walmes; Freitas,Rilke T. F.. |
| This study investigated the addition of antioxidants vitamin E and reduced glutathione on curimba (Prochilodus lineatus) semen cryopreservation and compared sodium bicarbonate solution and distilled water as activators. The experiment was conducted at the environmental station of CEMIG, in Itutinga-MG, Brazil, between December/2009 and January/2010. Semen samples (n = 7) with semen motility above 80% were diluted in cryoprotectant solutions composed of 10% methanol, 15% lactose and containing different concentrations of antioxidants: 50 (VE50), 100 (VE100) and 250 (VE250) µM of vitamin E, and 0.5 (RG0.5), 1.0 (RG1.0) and 1.5 (RG1.5) mM of reduced glutathione. A solution without antioxidants was used as a control. The semen was diluted at a ratio of 1:4... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidants; Fertilization; Freezing; Reproduction; Sperm motility. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000300020 |
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| Paula,Daniella A. J.; Machado,Mônica R. F.; Murgas,Luis D. S.; Rabelo,Denílson; Zangerônimo,Márcio G.; V. R. Neto,Rafael; Andrade,Estefânia S.; Andrade,Eliane A.. |
| This paper investigated the effect of cryoprotectant substances on Prochilodus lineatus embryos in an experimental incubator. The prospective study applied combinations of polyvinyl alcohol, hydroxyethyl cellulose, gelatin and fetal bovine serum with dimethyl sulfoxide and ethylene glycol in a new experimental incubator. The morphology of embryos, larval viability and the efficiency of experimental incubators in maintaining the quality of embryos were evaluated. This study demonstrates the efficient association between hydroxyethylcellulose and dimethyl sulfoxide as greater viability (p<0.05) was found for embryos (72.9 ± 23.9%). It should also be noted the permeation of cryoprotectants in embryos through the changes found in chorion diameter, embryo... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000400835 |
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