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Polyembryony and identification of Volkamerian lemon zygotic and nucellar seedlings using RAPD PAB
Andrade-Rodríguez,María; Villegas-Monter,Angel; Carrillo-Castañeda,Guillermo; García-Velázquez,Armando.
The objectives of this work were to evaluate the frequency of polyembryony, and to identify zygotic and nucellar seedlings of Citrus volkameriana using RAPD. Twenty-five polyembryonic and eight monoembryonic seeds were cultivated in vitrofor six months. DNA from seedlings was extracted and used in combination with five RAPD primers to identify zygotic or nucellar origin of the seedlings. Environmental conditions of the year affected significantly (P<0.05) the morphological characteristics of fruitsand the number ofembryos per seed. Polyembryonic seeds ranged from 30.9%, 44.8% to 54.4% over three years. Morphological characteristic was not correlated with polyembryony. In vitro culture enable all embryos of each seed to grow, favoring the percentage of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus volkameriana; Rootstock; Embryo culture; Molecular markers; Polymorphism.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000600006
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Secado y almacenamiento de semillas de mandarino 'Cleopatra' PAB
Villegas-Monter,Angel; Andrade-Rodríguez,María.
Los objetivos del trabajo fueron evaluar el efecto del tratamiento con productos químicos y contenido de humedad en la conservación de semillas de mandarino 'Cleopatra' (Citrus reshni Hort ex Tan). Semillas con 14,7% de humedad tratadas con cuatro funguicidas y 8-hidroxiquinoleina, fueron almacenadas a 4±2°C durante un año, al igual que tres lotes de semillas con 5,0%, 14,7% y 26,8% de humedad, tratadas con y sin 8-hidroxiquinoleina. Se evaluó la germinación y emergencia al inicio del experimento, a los seis y 12 meses de almacenamiento. En la prueba con productos químicos, el almacenamiento de semillas con 14,7% de humedad sólo fue viable hasta los seis meses, con mayores porcentajes de germinación y emergencia (59,3% y 54,0%, respectivamente), mientras...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus reshni; Emergencia de plántulas; Tratamiento de semillas con funguicidas; Germinación de semillas; 8-hidroxiquinoleina.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000100011
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Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars PAB
Ochoa,Elisa del Carmen Martínez; Andrade-Rodríguez,María; Rodríguez,Mario Rocandio; Monter,Angel Villegas.
The objective of this work was to evaluate the occurrence of polyembryony in the mango cultivars Manila and Ataulfo, and to determine whether seedlings cultured in vitro are zygotic or nucelar. Percentage of polyembryony was calculated and the number of embryos in 100 seeds of each cultivar was recorded. 'Manila' exhibited 97% polyembryony with 3.4 embryos per seed, while 'Ataulfo' had 95% polyembryony with 3.2 embryos per seed. Later, 20 seeds of each cultivar were established in vitro, and it was analyzed those in which all embryos germinated (12 seeds from 'Manila' and 7 from 'Ataulfo'). DNA was extracted from seedling leaf tissue, and its origin was identified with 14 RAPD primers. The polymorphic markers recognized the seedlings of sexual origin in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mangifera indica; Embryo culture; Funiculus; Molecular markers; Polymorphism.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012001100010
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