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| Johannes A. Schmid; Andreas Birbach. |
| IKK2 is one of the most crucial signaling kinases for activation of the transcription factor NF-kappa B. Since many NF-kappa B activating pathways converge at the level of IKK2, we searched for interaction partners of this kinase using the C-terminal part (aa 466-756) as bait in a yeast two-hybrid system. We identified the N-terminal part (aa 1-228) of the TNF-receptor associated factor TRAF1 as putative interaction partner, which was subsequently confirmed in mammalian cells by coimmunoprecipitation experiments. However, this interaction seemed weaker than the interaction between TRAF1 and TRAF2, an important activating adapter molecule of NF-kappa B signaling indicating that relative levels of IKK2, TRAF1 and TRAF2 might be important for the final... |
| Tipo: Manuscript |
Palavras-chave: Immunology; Molecular Cell Biology. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/4153/version/1 |
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| Kalsoom A. Sughra; Andreas Birbach; Rainer de Martin; Johannes A. Schmid. |
| *Background*
I-kappa B kinase 2 (IKK2 or IKK-beta) is one of the most crucial signaling kinases for activation of NF-kappa B, a transcription factor that is important for inflammation, cell survival and differentiation. Since many NF-kappa B activating pathways converge at the level of IKK2, molecular interactions of this kinase are pivotal for regulation of NF-kappa B signaling.

*Methodology/Principal Findings*
We searched for proteins interacting with IKK2 using the C-terminal part (amino acids 466-756) as bait in a yeast two-hybrid system and identified the N-terminal part (amino acids 1-228) of the TNF-receptor associated factor TRAF1 as putative interaction partner. The interaction... |
| Tipo: Manuscript |
Palavras-chave: Immunology; Molecular Cell Biology. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/4153/version/2 |
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