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	Provedor de dados BJM (1) Autor Andreolla,Ana Paula (1) Erpen,Luana Marina Scheer (1) Frandoloso,Rafael (1) Kreutz,Luiz Carlos (1) Palavra-chave Bovine leukemia virus (1) Diagnosis (1) ELISA (1) Retrovirus (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2018 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Development of an indirect ELISA based on recombinant capsid protein to detect antibodies to bovine leukemia virus BJM Andreolla,Ana Paula; Erpen,Luana Marina Scheer; Frandoloso,Rafael; Kreutz,Luiz Carlos. Abstract Serological testing and culling infected animals are key management practices aiming eradication of bovine leukemia virus infection. Here, we report the development of an indirect ELISA based on BLV recombinant capsid protein (BLVp24r) to detect anti-BLV antibodies in cattle serum. The BLVp24r was expressed in Escherichia coli and purified by affinity chromatography, and then used to set up the ELISA parameters. The Polysorp ® plate coated with 50 ng of antigen/well and bovine serum diluted 1:100 gave the best results during standardization. Using sera from infected and non-infected cattle we set up the cutoff point at 0.320 (OD450 nm) with a sensitivity of 98.5% and specificity of 100.0%. Then, we tested 1.187 serum samples from dairy (736... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine leukemia virus; Retrovirus; Diagnosis; ELISA. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500068 Registros recuperados: 1 Primeira ... 1 ... Última

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