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Reference range for T lymphocytes populations in blood donors from two different regions in Brazil BJID
Torres,A.J.L.; Angelo,A.L.D.; Netto,E.M.; Sampaio,G.P.; Souza,D.F.C.; Inocêncio,L.A.; Lemos,J.A.R.; Brites,C..
This study defined the normal variation range for different subsets of T-lymphocyte cells count in two different Brazilian regions. We analysed the T-lymphocytes subpopulations (CD3+, CD4+, CD8+) in blood donors of two Brazilian cities, located in North (Belem, capital state of Para, indian background) and Northeast (Salvador, capital state od Bahia, African background) regions of Brazil. Results were compared according to gender, stress level (sleep time lower than 8 hours/day), smoking, and alcohol intake. Lymphocytes subpopulations were measured by flow cytometry. Five hundred twenty-six blood donors from two Brazilians cities participated in the study: 450 samples from Bahia and 76 samples from Pará. Most (60%) were men, 59% reported alcohol intake,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CD4; CD8; Blood donors; Reference values; Brazil.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000300013
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Surfactant protein B gene polymorphism in preterm babies with respiratory distress syndrome BJMBR
Lyra,P.P.R.; Diniz,E.M.A.; Abe-Sandes,K.; Angelo,A.L.D.; Machado,T.M.B.; Cardeal,M..
The etiology of respiratory distress syndrome (RDS) is multifactorial and multigenic. Studies have suggested that polymorphisms and mutations in the surfactant protein B (SP-B) gene are associated with the pathogenesis of RDS. The objectives of this study were to determine and compare the frequencies of SP-B gene polymorphisms in preterm babies with and without RDS. We studied 151 neonates: 79 preterm babies without RDS and 72 preterm newborns with RDS. The following four SP-B gene polymorphisms were analyzed: A/C at -18, C/T at 1580, A/G at 9306, and G/C at nucleotide 8714. The polymorphisms were detected by PCR amplification of genomic DNA and genotyping. The genotypes were determined using PCR-based converted restriction fragment length polymorphisms....
Tipo: Info:eu-repo/semantics/other Palavras-chave: Newborn respiratory distress syndrome; Genetic polymorphism; Surfactant protein B.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000100010
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