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	Provedor de dados BJID (3) Autor Antunes,Ana Lúcia Souza (3) d'Azevedo,Pedro Alves (3) Perez,Leandro Reus Rodrigues (2) Secchi,Carina (2) Caierão,Juliana (1) Cantarelli,Vlademir Vicente (1) Gugel,Juliana (1) Martino,Marinês Dalla Vale (1) Pasternak,Jacyr (1) Siquiera,Itacy (1) Mais... Palavra-chave Antimicrobial susceptibility testing (1) Automated system (1) Cefoxitin (1) Clindamycin (1) Coagulase-negative Staphylococcus (1) D test (1) Gram-positive cocci (1) Identification (1) MecA gene (1) Oxacillin (1) Resistance (1) Susceptibility diagnostic (1) Vitek2 (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2009 (1) 2008 (1) 2007 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Identification and detection of methicillin resistance in Non-Epidermidis coagulase-negative staphylococci BJID Secchi,Carina; Antunes,Ana Lúcia Souza; Perez,Leandro Reus Rodrigues; Cantarelli,Vlademir Vicente; d'Azevedo,Pedro Alves. The NCCLS (2004) presented a new methodology to detect, by disk-diffusion agar, oxacillin-resistance using a cefoxitin disk. We identified coagulase-negative staphylococci (SCoN) to the species level and compared the use of cefoxitin disks (30 µg) with oxacillin disks (1 µg), agar dilution (minimum inhibitory concentration of oxacillin) and mecA gene detection in isolates of coagulase-negative bacteria other than Staphylococcus epidermidis (SCoNne). A total of 238 SCoNne was evaluated; oxacillin-resistance (the mecA gene) was detected in 71% of the isolates. All methods gave 100% sensitivity, based on presence of the mecA gene. The specificity of the cefoxitin disk was 100%, while the oxacillin disk gave a specificity of 91% and agar dilution oxacillin... Tipo: Info:eu-repo/semantics/article Palavras-chave: Coagulase-negative Staphylococcus; Cefoxitin; Oxacillin; MecA gene; Susceptibility diagnostic. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400012 Use of the D test method to detect inducible clindamycin resistance in coagulase negative staphylococci (CoNS) BJID Perez,Leandro Reus Rodrigues; Caierão,Juliana; Antunes,Ana Lúcia Souza; d'Azevedo,Pedro Alves. According to the National Committee for Clinical Laboratory Standards (NCCLS, 2004), a method to evaluate the inducible clindamycin resistance in accordance with an approach of the disks of erythromycin and clindamycin - the D test - has been reported. We analyzed the performance of this method in 200 coagulase negative staphylococci (CoNS) strains obtained from blood cultures of hospitalized patients at a general hospital in Southern Brazil. Twenty-seven clinical isolates with suitable profile (erythromycin-resistant and clindamycin-susceptible) were evaluated for the D test realization. Thus, only 5 CoNS were D test positive. The D test method showed to be simple and an important technique in the detection of inducible clindamycin resistance. Tipo: Info:eu-repo/semantics/article Palavras-chave: Clindamycin; Resistance; D test. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200002 Evaluation of the automated system Vitek2 for identification and antimicrobial susceptibility testing of Brazilian Gram-positive cocci strains BJID d'Azevedo,Pedro Alves; Siquiera,Itacy; Gugel,Juliana; Antunes,Ana Lúcia Souza; Secchi,Carina; Pasternak,Jacyr; Martino,Marinês Dalla Vale. Automated instruments offer many advantages for clinical laboratories. Nevertheless, they can have problems identifying and determining susceptibilities of some pathogens. Vitek® 2 (bioMérieux) is an automated system that was recently introduced to Brazil. We evaluated the performance of this equipment for Brazilian isolates that had been characterized using reference identification and antimicrobial susceptibility testing methods. Ninety-nine strains of Gram-positive cocci from a local reference center collection were analyzed, consisting of 50 coagulasenegative Staphylococcus (CoNS) and 49 Enterococcus and related species. Vitek® 2 correctly identified 79.8% (79/99) of the isolates. Oxacillin resistance was detected in 76% (19/25) of resistant S.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Automated system; Vitek2; Gram-positive cocci; Identification; Antimicrobial susceptibility testing. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000200007 Registros recuperados: 3 Primeira ... 1 ... Última

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