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| Elamri, D; Aouni, M; Parnaudeau, Sylvain; Le Guyader, Soizick. |
| Aims: The aim of this study was to detect the main pathogenic human RNA enteric viruses able to persist in the environment such as astrovirus, enterovirus, norovirus and hepatitis A virus (HAV) in shellfish collected from two locations in northern Tunisia. Methods and Results: Viruses were eluted from digestive tissues and concentrated by polyethylene glycol precipitation before nucleic acid extraction and purification. After checking for inhibitors, all viruses were detected by reverse transcription-polymerase chain reaction (RT-PCR) and confirmed by hybridization. Overall, 83% of the samples were found positive for at least one virus. Astrovirus was detected in 61% of the samples, norovirus in 35% and HAV in 26%. Surprisingly, only one sample was found... |
| Tipo: Text |
Palavras-chave: Shellfish; Reverse transcription PCR; Human enteric viruses. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1881.pdf |
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