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| Ramos,Carlos A.N.; Araújo,Flábio R.; Santos,Rafaelle C.; Melo,Elaine S.P.; Sousa,Letícia C.; Vidal,Carlos E.S.; Guerra,Neurisvan R.; Ramos,Rafael A.N.. |
| The recombinant protein MSP5 has been established as an important antigen for serological diagnosis of Anaplasma marginale by enzyme-linked immunosorbent assay (ELISA). However, due to the high cost of specialized equipment, this technique is not accessible to all laboratories, especially in developing countries in areas where the disease is endemic. The present study describes the standardization of a latex agglutination test (LAT) to detect antibodies against A. marginale based on recombinant MSP5. Compared with indirect enzyme-linked immunosorbent assay (iELISA), the relative sensitivity and specificity of the LAT were 95.21% and 91.86% respectively, with an almost perfect agreement between tests (kappa index = 0.863). These results can be considered... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Latex agglutination; Rapid test; Anaplasma marginale; MSP5. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100026 |
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| Araújo,Cristina P.; Osório,Ana Luiza A.R.; Jorge,Klaudia S.G.; Ramos,Carlos A.N.; Souza Filho,Antonio F.; Vidal,Carlos E.S.; Vargas,Agueda P.C.; Roxo,Eliana; Rocha,Adalgiza S.; Suffys,Philip N.; Fonseca Júnior,Antônio A.; Silva,Marcio R.; Barbosa Neto,José D.; Cerqueira,Valíria D.; Araújo,Flábio R.. |
| Post-mortem bacterial culture and specific biochemical tests are currently performed to characterize the etiologic agent of bovine tuberculosis. Cultures take up to 90 days to develop. A diagnosis by molecular tests such as PCR can provide fast and reliable results while significantly decreasing the time of confirmation. In the present study, a nested-PCR system, targeting rv2807, with conventional PCR followed by real-time PCR, was developed to detect Mycobacterium tuberculosis complex (MTC) organisms directly from bovine and bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other Actinomycetales species and DNA samples... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine and bubaline tuberculosis; Nested-PCR; Real-time PCR; Tissue; Sanitary inspection. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200035 |
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