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| Raffoul,Tássia; Swiech,Kamilla; Arantes,Mabel Karina; Sousa,Álvaro Paiva Braga de; Mendonça,Ronaldo Zucatelli; Pereira,Carlos Augusto; Suazo,Cláudio Alberto Torres. |
| The aim of this work was to evaluate the potential of hemolymph utilization as a culture medium supplement to cultivate the animal cell CHO-K1. For this purpose 1% v/v of hemolymph was added to DMEM medium containing 10% v/v of FBS and 1 or 4.5 g/L of glucose. The culture was grown in spinner flasks incubated in a 10% v/v CO2 environment, at 37ºC, with the Cytodex 1 microcarrier. Comparing the results obtained from the culture with hemolymph against those without hemolymph, a positive influence of the hemolymph was observed, as the experiment with hemolymph presented a 52% higher cell concentration and a higher productivity of up to 40%. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Animal cell; CHO-K1; Microcarrier; Growth; Attachment; Hemolymph. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400011 |
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| Feroldi,Michael; Caroline,Andressa; Borba,Neves, Carlos Eduardo; Arantes,Mabel Karina; José,Alves Helton. |
| ABSTRACT The global use of fossil energy sources has led to the need to develop technologies capable of mitigating its consequences. One such option is anaerobic biodigestion, which is already scientifically well-established, although the use of its main products (biogas and biomethane) still requires further development before they can be employed in automotive vehicles, largely due to technical issues concerning their storage. The storage of methane using adsorption (Adsorbed Natural Gas - ANG) is a technology that requires less energy and moderate pressures and temperatures. However, the main storage techniques continue to be those that involve high pressures and/or cryogenic temperatures. The present work evaluates the performance of an unprecedented... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adsorption; Biogas; Vehicle fuel. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200200 |
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