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Improvement in nutritional quality of fibrous food via in vitro digestion by Aspergillus niger Ciencia e Investigación Agraria
Velásquez,Alejandro; Marnet,Pierre-Guy; Arias,Rodrigo.
In a first experiment, the effect of in vitro incubation with Aspergillus niger (An) on the chemical composition of different fibrous substrates was studied. In a second experiment, the effect of incubation time (0,72 and 144 h) on in vitro digestion with An of dry matter (IVDMD), neutral detergent fiber (DVNDF) and acid detergent fiber (DVADF) was evaluated for different substrates. Wheat straw, WS; barley straw, BS; oat hulls, OH; dehydrated alfalfa, DA; and dehydrated ryegrass, DB substrates were evaluated. In both experiments, incubations were performed in 250 mL Erlenmeyer flasks; 2 g of substrate was added to a culture medium (pH = 6), and incubated at 28 °C with constant ventilation. The An dose consisted of 3 mL of a solution of 5.3 χ...
Tipo: Journal article Palavras-chave: Aspergillus niger; Fibrous substrates; In vitro digestion; Nutritional quality.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202015000100005
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Effect of the type of substrate on the chemical composition and productivity of a protein concentrate of yeast origin Ciencia e Investigación Agraria
Velásquez,Alejandro; Arias,Rodrigo; Toneatti,Marcelo.
Extracts of Saccharomyces cerevisiae (Sc) yeast were generated using a biotechnological solid-state fermentation method. The organic material used for fermentation consisted of a variety of fibrous substrates of agricultural origin, and their effect on the chemical composition and productivity of cellular biomass generated was evaluated. The substrates studied included the following: wheat straw, WS; barley straw, BS; chili stubble, CS; oat hull, OH and starch powder, SP (control). For incubation with Sc, 2 g of (dry) vegetable substrate were added to 12 mL of cultivation medium. The fermentation time was seven days under aerobic conditions (pH 5, 26°C). After extraction of the yeast biomass, its chemical composition and productivity were analyzed. The...
Tipo: Journal article Palavras-chave: Protein concentrate; Saccharomyces cerevisiae; Vegetable substrate.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300003
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Effect of substrate on the in vitro protein digestibility of extracts generated by Saccharomyces cerevisiae Ciencia e Investigación Agraria
Velásquez,Alejandro; Arias,Rodrigo.
A. Velásquez, and R. Arias. 2013. Effect of substrate on the in vitro protein digestibility of extracts generated by Saccharomyces cerevisiae. Cien. Inv. Agr. 40(3): 503-511. The in vitro crude protein (CP) digestibility (Divcp), true protein digestibility (Dtp) and degradation rate (kd cp) were measured in different protein extracts of Saccharomyces cerevisiae (Sc) yeast. These extracts were generated through a biotechnological method (solid state fermentation). Wheat straw (TWS), barley straw (TBS), chili stubble (TCS), oats hull (TOH) and starch-glucose powder (TSP; control) were used as substrates for fermentation by Sc, and their effect on the aforementioned kinetic parameters in the generated cellular biomass was evaluated. In 12 mL cultivation...
Tipo: Journal article Palavras-chave: In vitro protein digestibility; Protein extract; Saccharomyces cerevisiae; Vegetable substrates.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000300004
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