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| Simoni,Isabela Cristina; Fernandes,Maria Judite Bittencourt; Custódio,Renata Marconi; Madeira,Alda Maria Backx Noronha; Arns,Clarice Weis. |
| The susceptibility of the five cell lines - IB-RS-2, RK-13, Vero, BHK-21, CER - to reovirus S1133 and infectious bursal disease virus (IBDV vaccine GBV-8 strain) was studied to better define satisfactory and sensitive cell culture systems. Cultures were compared for presence of CPE, virus titers and detection of viral RNA. CPE and viral RNA were detected in CER and BHK-21 cells after reovirus inoculation and in RK-13 cell line after IBDV inoculation and with high virus titers. Virus replication by production of low virus titers occurred in IB-RS-2 and Vero cells with reovirus and in BHK-21 cell line with IBDV. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Reovirus; Infectious bursal disease virus; Cell lines; Susceptibility. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400015 |
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| Riccetto,Adriana Gut Lopes; Silva,Luciana Helena Antoniassi da; Spilki,Fernando Rosado; Morcillo,André Moreno; Arns,Clarice Weis; Baracat,Emílio Carlos Elias. |
| The aim of this study was to determine if there was a correlation between respiratory syncytial virus (RSV) and metapneumovirus (MPV) genotypes and clinical data of Brazilian infants hospitalized for acute lower respiratory infection. The viruses in the patients' nasopharyngeal secretions were studied using the polymerase chain reaction and phylogenetic analysis. The study assessed 144 infants; 31.9% were RSV positive and 5.6% were MPV positive. Statistical analysis was performed using the chi-squared test, Fisher's test, Odds ratio, univariate logistic regression, non-conditional multivariate logistic regression and the forward - stepwise method. Multivariate analysis confirmed a significant relationship between a positive PCR test for RSV and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Respiratory syncytial virus; Metapneumovirus; Genotypes; Infants; Clinical. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000100008 |
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| Bueno,Ieda Aparecida Correa; Riccetto,Adriana Gut Lopes; Morcillo,André Moreno; Arns,Clarice Weis; Baracat,Emílio Carlos Elias. |
| The aims of this study were to determine the presence of respiratory syncytial virus (RSV) and to assess the clinical features of the disease in infants with acute low respiratory tract infection hospitalized at pediatric intensive care units (PICU) of two university teaching hospitals in São Paulo State, Brazil. Nasopharyngeal secretions were tested for the RSV by the polymerase chain reaction. Positive and negative groups for the virus were compared in terms of evolution under intensive care (mechanical pulmonary ventilation, medications, invasive procedures, complications and case fatality). Statistical analysis was performed using the Mann Whitney and Fisher's exact tests. A total of 21 infants were assessed, 8 (38.1%) of whom were positive for RSV.... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Respiratory syncytial virus; Infants; Intensive therapy; Genotypes. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000100016 |
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| Fernandes,Maria Judite Bittencourt; Simoni,Isabela Cristina; Harakava,Ricardo; Rivas,Eliana Borges; Arns,Clarice Weis. |
| Infectious bursal disease virus (IBDV) is classified according to the antigenicity and virulence into classical virulent (cv), very virulent (vv), and antigenic variant strains. The molecular basis for the IBDV antigenic variation is well established and is associated to the capsid protein, VP2 (gene VP2 of segment A), whereas both VP2 and the RNA-dependent RNA polymerase, VP1 (gene VP1 of segment B), have been correlated with the virulence. In this study, seventeen Brazilian IBDV samples previously characterized by the VP2 gene as cv (three) and vv (fourteen) strains were genetically and molecularly analyzed for their VP1 gene. All of the strains kept with the same cv or vv classification except one sample, Br/03/DR. This sample was classified as vv by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brazilian infectious bursal disease virus; Very virulent strain; VP1; Sequencing; Phylogenetic analysis. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300024 |
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