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Variety discrimination of Tigridia pavonia (L.f.) DC. assesed by different length RAPD primers Electron. J. Biotechnol.
Piña-Escutia,José Luis; Vázquez-García,Luis Miguel; Arzate-Fernández,Amaury Martín.
Tigridia pavonia (L.f.) DC. is one of the important phytogenetic resources of México. This species is used as ornamental, food and medicinal purposes. Despite its ornamental and economic potential, there is little information about the genetic variability. In this study, randomly amplified polymorphic DNA (RAPD) primers of 10, 15 and 20 bases were used to assess the level of genetic variation among nine botanical varieties of Tigridia pavoniacollected in three localities within State of México. The total number fragments, polymorphic fragments, percentage of polymorphism and resolving power were greater for 15 base (55, 52, 94.5 and 5, respectively) and 20 base (47, 45, 95.7 and 3.8, respectively), in comparison with those obtained from 10 base primers...
Tipo: Journal article Palavras-chave: Genetic resources; RAPD; Tigridia pavonia; Variety discrimination.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400010
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In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC. Electron. J. Biotechnol.
Piña-Escutia,José Luis; Vázquez-García,Luis Miguel; Arzate-Fernández,Amaury Martín.
Plants of Tigridia pavonia (L.f.) DC were regenerated from twin-scaling explants cultured on Murashige and Skoog and N6 basal medium. The highest formation of shoots per responding explant was obtained on N6 medium supplemented with 4.5 µM 2,4-dichlorophenoxy acetic acid in combination with 2.2 µM benzylaminopurine. Shoots rooted readily on N6 basal medium supplemented with 1 g l-1 activated charcoal and 2.6 µM naphtalenacetic acid. The rooted shoots achieved 100% survival. Inter Simple Sequence Repeat analysis was carried out to check for possible genetic alterations in plants obtained after two consecutive subcultures. The results revealed that the recovered plants did not exhibit any type of polymorphism.
Tipo: Journal article Palavras-chave: Genetic integrity; Micropropagation; Oceloxóchitl.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100007
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